Toxicity of Cypermethrin and Fenitrothion on the Hemolymph Carbohydrates, Head Acetylcholinesterase, and Thoracic Muscle Na+, K+-ATPase of Emerging Honeybees (Apis mellifera mellifera. L)

Bendahou, Najib; Bounias, M.; Fléché, C.

doi:10.1006/eesa.1999.1811

Cited by 43 publications

(27 citation statements)

References 27 publications

(31 reference statements)

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“…Fed controls and not fed controls did not present any signi"cant di!erence in their EFFECT OF CYPERMETHRIN ON HONEYBEE COLONIES trehalosemia between Weeks 10}19. The same phenomena of hypoglucosemia and hypotrehalosemia have been observed in another laboratory with cypermethrin (Bendahou et al, 1997).…”

Section: Resultssupporting

confidence: 80%

Biological and Biochemical Effects of Chronic Exposure to Very Low Levels of Dietary Cypermethrin (Cymbush) on Honeybee Colonies (Hymenoptera: Apidae)

Bendahou

Fléché

Bounias

1999

Ecotoxicology and Environmental Safety

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Section: Resultssupporting

confidence: 80%

Biological and Biochemical Effects of Chronic Exposure to Very Low Levels of Dietary Cypermethrin (Cymbush) on Honeybee Colonies (Hymenoptera: Apidae)

Bendahou

Fléché

Bounias

1999

Ecotoxicology and Environmental Safety

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“…Gene expression can be manipulated by injecting double stranded RNA (Schlüns and Crozier, 2007; see also Section VI -RNA interference). Injections of pathogens (Wilson and Rothenbuhler, 1968) and insecticides (Bendahou et al, 1999), as well as injection of labelled markers to trace substance distributions (Crailsheim, 1992) are further applications.…”

Section: Introductionmentioning

confidence: 99%

Miscellaneous standard methods forApis melliferaresearch

Human

Brodschneider

Dietemann

et al. 2013

Journal of Apicultural Research

227

138

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SummaryA variety of methods are used in honey bee research and differ depending on the level at which the research is conducted. On an individual level, the handling of individual honey bees, including the queen, larvae and pupae are required. There are different methods for the immobilising, killing and storing as well as determining individual weight of bees. The precise timing of developmental stages is also an important aspect of sampling individuals for experiments. In order to investigate and manipulate functional processes in honey bees, e.g. memory formation and retrieval and gene expression, microinjection is often used. A method that is used by both researchers and beekeepers is the marking of queens that serves not only to help to locate her during her life, but also enables the dating of queens. Creating multiple queen colonies allows the beekeeper to maintain spare queens, increase brood production or ask questions related to reproduction. On colony level, very useful techniques are the measurement of intra hive mortality using dead bee traps, weighing of full hives, collecting pollen and nectar, and digital monitoring of brood development via location recognition. At the population level, estimation of population density is essential to evaluate the health status and using beelines help to locate wild colonies. These methods, described in this paper, are especially valuable when investigating the effects of pesticide applications, environmental pollution and diseases on colony survival. Métodos estándar diversos para la investigación en Apis mellifera ResumenEn la investigación de la abeja de la miel, se han usado una variedad de métodos que se diferencian en función del nivel en el que se realiza la investigación. Al nivel individual, el manejo de las abejas individuales es necesario, incluyendo a la reina, las larvas y las pupas. Existen diferentes métodos para la inmovilización, mortandad y almacenamiento, así como para la determinación del peso individual de las abejas. La precisión en la sincronización de las etapas de desarrollo es también un aspecto importante de los experimentos con muestreos individuales.La microinyección se utiliza a menudo con el fin de investigar y manipular los procesos funcionales de las abejas melíferas, como por ejemplo, la formación y recuperación de la memoria y la expresión génica. Un método utilizado tanto por investigadores como apicultores es el marcado de las reinas, que sirve no sólo para ayudar a localizarlas durante su vida, sino que también permite su datación. La creación de varias colmenas a partir de reinas permite al apicultor mantener reinas de repuesto, aumentar la producción de cría o hacer preguntas relacionadas con la reproducción. Al nivel de colmena, la medición de la mortalidad intra colmena utilizando trampas de abejas muertas, el pesaje de las colmenas completas, la recolección de polen y néctar, y el seguimiento digital del desarrollo de la cría a través del reconocimiento de su ubicación, son algunas de las técnicas más útiles. Al nivel p...

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“…In one study (35) deltamethrin was lethal to worker bees and reduced their foraging activity at food source and hive entrance activity. In another study (152), cypermethrin inhibited Na/K ATPase in emerging honeybees in laboratory conditions. In caged honeybees it altered the expression of the genes involved in the immune system, cytochrome P450 enzymes, subunits of nAChRs, and vitellogenin (153).…”

Section: Pyrethroid Effects On Non-target Insects and Invertebratesmentioning

confidence: 90%

Non-target toxicity of novel insecticides

Mužinić

Želježić

2018

Archives of Industrial Hygiene and Toxicology

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Humans have used insecticides since ancient times. The spectrum and potency of available insecticidal substances has greatly expanded since the industrial revolution, resulting in widespread use and unforeseen levels of synthetic chemicals in the environment. Concerns about the toxic effects of these new chemicals on non-target species became public soon after their appearance, which eventually led to the restrictions of use. At the same time, new, more environmentally-friendly insecticides have been developed, based on naturally occurring chemicals, such as pyrethroids (derivatives of pyrethrin), neonicotinoids (derivatives of nicotine), and insecticides based on the neem tree vegetable oil (Azadirachta indica), predominantly azadirachtin. Although these new substances are more selective toward pest insects, they can still target other organisms. Neonicotinoids, for example, have been implicated in the decline of the bee population worldwide. This review summarises recent literature published on non-target toxicity of neonicotinoids, pyrethroids, and neem-based insecticidal substances, with a special emphasis on neonicotinoid toxicity in honeybees. We also touch upon the effects of pesticide combinations and documented human exposure to these substances.

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Toxicity of Cypermethrin and Fenitrothion on the Hemolymph Carbohydrates, Head Acetylcholinesterase, and Thoracic Muscle Na+, K+-ATPase of Emerging Honeybees (Apis mellifera mellifera. L)

Cited by 43 publications

References 27 publications

Biological and Biochemical Effects of Chronic Exposure to Very Low Levels of Dietary Cypermethrin (Cymbush) on Honeybee Colonies (Hymenoptera: Apidae)

Biological and Biochemical Effects of Chronic Exposure to Very Low Levels of Dietary Cypermethrin (Cymbush) on Honeybee Colonies (Hymenoptera: Apidae)

Miscellaneous standard methods forApis melliferaresearch

Non-target toxicity of novel insecticides

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