Toxicity of common biocides used in aquaculture to embryos and larvae of Brachymystax tsinlingensis Li

Abstract

Brachymystax tsinlingensis Li is a threatened fish species endemic to China. With the problems of environmental factors and seeding breeding diseases, it is important to further improve the efficiency of seeding breeding and the basis of resource protection. This study investigated the acute toxicity of copper, zinc and methylene blue (MB) on hatching, survival, morphology, heart rate (HR) and stress behaviour of B. tsinlingensis. Eggs (diameter: 3.86 ± 0.07 mm, weight: 0.032 ± 0.004 g) of B. tsinlingensis were selected randomly from artificial propagation and developed from eye‐pigmentation‐stage embryos to yolk‐sac stage larvae (length: 12.40 ± 0.02 mm, weight: 0.03 ± 0.001 g) and exposed to different concentrations of Cu, Zn and MB for 144 h in a series of semi‐static toxicity tests. The acute toxicity tests indicated that the 96‐h median lethal concentration (LC50) values of the embryos and larvae were 1.71 and 0.22 mg l−1 for copper and 2.57 and 2.72 mg l−1 for zinc, respectively, whereas the MB LC50 after 144‐h exposure for embryos and larvae were 67.88 and 17.81 mg l−1, respectively. The safe concentrations of copper, zinc and MB were 0.17, 0.77 and 6.79 mg l−1 for embryos and 0.03, 0.03 and 1.78 mg l−1 for larvae, respectively. Copper, zinc and MB treatments with concentrations greater than 1.60, 2.00 and 60.00 mg l−1, respectively, led to a significantly low hatching rate and significantly high embryo mortality (P < 0.05), and copper and MB treatments with concentrations greater than 0.2 and 20 mg l−1 led to significantly high larvae mortality (P < 0.05). Exposure to copper, zinc and MB resulted in developmental defects, including spinal curvature, tail deformity, vascular system anomalies and discolouration. Moreover, copper exposure significantly reduced the HR of larvae (P < 0.05). The embryos exhibited an obvious change in behaviour, converting from the normal behaviour of emerging from the membrane head first to emerging tail first, with probabilities of 34.82%, 14.81% and 49.07% under copper, zinc and MB treatments, respectively. The results demonstrated that the sensitivity of yolk‐sac larvae to copper and MB was significantly higher than that of embryos (P < 0.05) and that B. tsinlingensis embryos or larvae might be more resistant to copper, zinc and MB than other members of the Salmonidae family, which benefits their resource protection and restoration.

Disparities in Body Color Adaptability and Ambient Light Color Preference between Wild and Hatchery-Reared Marbled Rockfish (Sebastiscus marmoratus)

“…Additionally, there appears to be discernible variance in the visual photosensitive function between hatchery-reared and wild individuals. Notably, research on the second-generation population of hatchery-reared Chinese lenok Brachymystax tsinlingensis revealed a significantly higher count of cone cells in the retina, indicative of heightened spectral sensitivity among hatchery-reared individuals [ 24 ]. These physiological disparities may influence the perception of environmental light among hatchery-reared individuals, potentially leading to distinct phototaxis behavioral phenotypes.…”

Research advances in diversity and conservation science of freshwater fish in China

“…He et al (2024) investigated the key life‐history traits of a critically endangered freshwater fish Ptychidio jordani , which had not been known previously and was essential for conservation strategy and policy development. Guo, Chu, et al (2024) provided the safe concentrations of the common biocides, including copper, zinc, and methylene blue to treat the disease of the threatened fish species that are endemic to China such as Brachymystax tsinlingensis , which benefited from resource protection and restoration measures.…”