Toxicity of cobalt oxide nanoparticles to normal cells; an in vitro and in vivo study

Chattopadhyay, Sourav; Dash, Sandeep Kumar; Tripathy, Satyajit; Das, Balaram; Mandal, Debasis; Pramanik, Panchanan; Roy, Somenath

doi:10.1016/j.cbi.2014.11.016

Cited by 120 publications

(75 citation statements)

References 41 publications

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“…To particle size and distribution characterization, CoFe 2 O 4 -NPs were suspended in Milli-Q water and cell culture medium with 10% FBS, and measured by transmission electron microscopy (TEM) (Jem-2100 HR, JEOL, USA). [15][16][17] The average hydrodynamic size of CoFe 2 O 4 -NPs in cell culture medium was determined by dynamic light scattering (DLS) (ZetaSizer Nano-ZS, Malvern Instruments, Malvern, UK). One mg CoFe 2 O 4 -NPs was dispersed in cell culture medium, and then the suspension was sonicated at room temperature for 15 min at 40 W. Ten μL of the suspension were diluted with cell culture medium to reach final concentration 10 μg/mL, and sonicated for further 5 min.…”

Section: Introductionmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Asadi

Gurkaynak

Özhan

2017

tjps

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ÖZAmaç: Sert manyetik kobalt ferrit nanopartiküllerinin (CoFe 2 O 4 -NP) dikkate değer özellikleri ve fizikokimyasal kararlılıkları farklı endüstri ve tıp alanlarında çeşitli uygulamalarda kullanılmalarına yol açmaktadır. CoFe 2 O 4 -NP'lerin bazı toksik etkilere neden olduğu bildirilmiş olsa da böbrek üzerindeki etkileri hakkında ciddi bilgi eksikliği vardır. Bu çalışmada, CoFe 2 O 4 -NPs'lerinin NRK-52E böbrek hücreleri üzerine toksik etki potansiyellerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Partikül karakterizasyonu ve hücresel alım transmisyon elektron mikroskopu, dinamik ışık saçılma tekniği ve indüktif eşleştirilmiş plazma-kütle spektrometrisi ile gerçekleştirildi. Sonra, sitotoksisite MTT ve nötral kırmızı alım testi, genotoksisite comet tekniği ve apoptotik potansiyel propidyum iyodürlü Annexin V-FITC apoptoz tayini ile değerlendirildi. Bulgular: CoFe 2 O 4 -NP'lere (39±17 nm) 100-1000 μg/mL arasında değişen konsantrasyonlarda 24 saat süre ile maruz bırakılan böbrek hücrelerinde hücre canlılığının etkilenmediği, ancak ≤100 μg/mL'de önemli ölçüde DNA hasarı meydana geldiği gözlenmiştir. Maruz kalan hücrelerde apoptotik veya nekrotik etki gözlenmedi. Sonuç: Elde edilen sonuçlara göre, CoFe 2 O 4 -NP'ler çeşitli uygulamalarda güvenli kullanımı vaat etmektedir. Bununla birlikte, etki mekanizmalarının tam olarak anlaşılabilmesi için in vivo çalışmalara ihtiyaç vardır. Anahtar kelimeler: DNA hasarı, hücre ölümü, apoptoz, kobalt ferrit nanopartikülü Objectives: The remarkable properties of hard magnetic cobalt ferrite nanoparticles (CoFe 2 O 4 -NPs) and their physicochemical stability lead to various applications in different industrial and medical fields. Although CoFe 2 O 4 -NPs have been reported to cause toxic effects, there is a serious lack of information concerning their effects on the kidneys. In this study, it was aimed to investigate the toxic effects of CoFe 2 O 4 -NPs on NRK-52E kidney cells. Materials and Methods: The particle characterisation and cellular uptake were determined using transmission electron microscopy, dynamic light scattering and inductively coupled plasma-mass spectrometry. Then, the cytotoxicity was evaluated by MTT and neutral red uptake assays, the genotoxicity by comet assay, and the apoptotic potentials by Annexin V-FITC apoptosis detection assay with propidium iodide. Results: After 24 h exposure to CoFe 2 O 4 -NPs (39±17 nm), it was observed they did not affect the cell viability at concentration ranging from 100 to 1000 μg/mL, but significantly induced DNA damage at concentration ≤100 μg/mL. No apoptotic or necrotic effect was observed in the exposed cells. Conclusion: According to the results obtained, CoFe 2 O 4 -NPs are promising for safe use in various applications. However, further in vivo studies are needed to fully understand their mechanisms of action.

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Section: Introductionmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Asadi

Gurkaynak

Özhan

2017

tjps

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“…Co3O4 nanoparticles, synthesized by thermal decomposition, exert oxidative stress on human lymphocytes, damage DNA, and cause inflammatory responses [70]. Oxidative stress is an important factor for toxicity and causes the induction of apoptosis.…”

Section: Cobalt Oxide (Co3o4) Nanocrystalsmentioning

confidence: 99%

“…Oxidative stress is an important factor for toxicity and causes the induction of apoptosis. The authors assumed that Co 2+ ions, when released from cobalt oxide nanoparticles, are the primary source of toxicity through the induction of TNF--caspase-8-p38-caspase-3 in immune cells [70]. Co3O4 nanoparticles induced cytotoxicity, morphological transformation, and genotoxicity in Balb3T3 cells [71,72].…”

Section: Cobalt Oxide (Co3o4) Nanocrystalsmentioning

confidence: 99%

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Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

179

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This review discusses recent advances in the synthesis, characterization and toxicity of metal oxide nanoparticles obtained mainly through biogenic (green) processes. The in vitro and in vivo toxicities of these oxides are discussed including a consideration of the factors important for safe use of these nanomaterials. The toxicities of different metal oxide nanoparticles are compared. The importance of biogenic synthesized metal oxide nanoparticles has been increasing in recent years; however, more studies aimed at better characterizing the potent toxicity of these nanoparticles are still necessary for nanosafely considerations and environmental perspectives. In this context, this review aims to inspire new research in the design of green approaches to obtain metal oxide nanoparticles for biomedical and technological applications and to highlight the critical need to fully investigate the nanotoxicity of these particles. OPEN ACCESSMetals 2015, 5 935

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“…It was reported that occupational exposure to CoNPs has been associated with adverse health effects including rhinitis, asthma, allergic dermatitis, and cardiomyopathy [26]. As the previous studies have been reported, As it is well known, nanoparticle toxicity is still controversial and depends on cell type sensitivity, method and condition of exposure, as well as nanoparticle characterisation [27][28]. In addition, there have been no studies concerning the nephrotoxicity of Co 3 O 4 nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Cellular Responses in Kidney Cells Exposed to Cobalt Oxide Nanoparticles

Asadi¹,

Gurkaynak²,

Özhan³

2017

Marmara Pharmaceutical Journal

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Cobalt oxide (Co 3 O 4 ) nanoparticles have been extensively used in various industrial and medical applications due to their special optical, magnetic, and electrical activity features. However, there is a lack of information about their toxicity and adverse effects on human health, especially concerning the kidney, which is considered to be a secondary target organ. We investigated the toxic potentials of Co 3 O 4 nanoparticles on NRK-52E kidney epithelial cells by in vitro assays. Co 3 O 4 nanoparticles were taken up by the kidney cells, and caused a decrease in cell viability, by significantly inducing apoptosis/ necrosis at 100 µg/mL. However, no significant DNA damage was observed. Co 3 O 4 nanoparticles induced cellular toxicity in kidney cells. These results should raise concern about the safety of Co 3 O 4 nanoparticles in their various applications. Further studies are needed to elucidate their toxic mechanism.

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Toxicity of cobalt oxide nanoparticles to normal cells; an in vitro and in vivo study

Cited by 120 publications

References 41 publications

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Nanotoxicology of Metal Oxide Nanoparticles

Assessment of Cellular Responses in Kidney Cells Exposed to Cobalt Oxide Nanoparticles

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