Toxicity of cobalt ferrite (CoFe2O4) nanobeads in Chlorella vulgaris: Interaction, adaptation and oxidative stress

Ahmad, Farooq; Yao, Hongzhou; Liu, Xiaoyi

doi:10.1016/j.chemosphere.2015.08.008

Cited by 40 publications

(31 citation statements)

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“…23 It was reported the interaction between CoFe 2 O 4 -NPs and nucleic acid occurred, and the linkage was based on a coordination interaction of the phosphate groups and the oxygen atoms on the heterocyclic bases of DNA with metal ions on the particle surface. 24 Also, Ahmad et al 10 pointed out the genotoxicity of CoFe 2 O 4 -NPs. Similarly, Colognato et al 25 reported the induction of genotoxicity in human peripheral lymphocytes exposed those CoFe 2 O 4 -NPs.…”

Section: Discussionmentioning

confidence: 99%

“…[7][8][9] However, some researchers have shown that CoFe 2 O 4 -NPs could cause oxidative damage, cell death and inflammatory responses in exposed mice, guinea pigs, zebrafish and human cell lines. [10][11][12][13][14] Therewith, both in vitro and in vivo studies should be gradually carried out to get comprehensive toxicity profiles of nanoparticles to predict their effects on human. There is no study evaluating the effects of CoFe 2 O 4 -NPs or any other cobalt based nanoparticle on kidney.…”

Section: Introductionmentioning

confidence: 99%

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<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Asadi

Gurkaynak

Özhan

2017

tjps

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ÖZAmaç: Sert manyetik kobalt ferrit nanopartiküllerinin (CoFe 2 O 4 -NP) dikkate değer özellikleri ve fizikokimyasal kararlılıkları farklı endüstri ve tıp alanlarında çeşitli uygulamalarda kullanılmalarına yol açmaktadır. CoFe 2 O 4 -NP'lerin bazı toksik etkilere neden olduğu bildirilmiş olsa da böbrek üzerindeki etkileri hakkında ciddi bilgi eksikliği vardır. Bu çalışmada, CoFe 2 O 4 -NPs'lerinin NRK-52E böbrek hücreleri üzerine toksik etki potansiyellerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Partikül karakterizasyonu ve hücresel alım transmisyon elektron mikroskopu, dinamik ışık saçılma tekniği ve indüktif eşleştirilmiş plazma-kütle spektrometrisi ile gerçekleştirildi. Sonra, sitotoksisite MTT ve nötral kırmızı alım testi, genotoksisite comet tekniği ve apoptotik potansiyel propidyum iyodürlü Annexin V-FITC apoptoz tayini ile değerlendirildi. Bulgular: CoFe 2 O 4 -NP'lere (39±17 nm) 100-1000 μg/mL arasında değişen konsantrasyonlarda 24 saat süre ile maruz bırakılan böbrek hücrelerinde hücre canlılığının etkilenmediği, ancak ≤100 μg/mL'de önemli ölçüde DNA hasarı meydana geldiği gözlenmiştir. Maruz kalan hücrelerde apoptotik veya nekrotik etki gözlenmedi. Sonuç: Elde edilen sonuçlara göre, CoFe 2 O 4 -NP'ler çeşitli uygulamalarda güvenli kullanımı vaat etmektedir. Bununla birlikte, etki mekanizmalarının tam olarak anlaşılabilmesi için in vivo çalışmalara ihtiyaç vardır. Anahtar kelimeler: DNA hasarı, hücre ölümü, apoptoz, kobalt ferrit nanopartikülü Objectives: The remarkable properties of hard magnetic cobalt ferrite nanoparticles (CoFe 2 O 4 -NPs) and their physicochemical stability lead to various applications in different industrial and medical fields. Although CoFe 2 O 4 -NPs have been reported to cause toxic effects, there is a serious lack of information concerning their effects on the kidneys. In this study, it was aimed to investigate the toxic effects of CoFe 2 O 4 -NPs on NRK-52E kidney cells. Materials and Methods: The particle characterisation and cellular uptake were determined using transmission electron microscopy, dynamic light scattering and inductively coupled plasma-mass spectrometry. Then, the cytotoxicity was evaluated by MTT and neutral red uptake assays, the genotoxicity by comet assay, and the apoptotic potentials by Annexin V-FITC apoptosis detection assay with propidium iodide. Results: After 24 h exposure to CoFe 2 O 4 -NPs (39±17 nm), it was observed they did not affect the cell viability at concentration ranging from 100 to 1000 μg/mL, but significantly induced DNA damage at concentration ≤100 μg/mL. No apoptotic or necrotic effect was observed in the exposed cells. Conclusion: According to the results obtained, CoFe 2 O 4 -NPs are promising for safe use in various applications. However, further in vivo studies are needed to fully understand their mechanisms of action.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Asadi

Gurkaynak

Özhan

2017

tjps

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“…However little is known about the in vivo fate of nanoparticles metallic dopants such as cobalt. The potential toxicity of CoIONPs is a subject of debate with regards to environmental, health and safety issues connected to their application in nanomedicine212223. Natural organic matter as well as cellular media can promote degradation of CoIONPs resulting in possible release of Co 2+ and Fe 3+ /Fe 2+ into the environment2425.…”

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confidence: 99%

Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin

Volatron

Kolosnjaj‐Tabi

Javed

et al. 2017

Sci Rep

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Metallic nanoparticles have been increasingly suggested as prospective therapeutic nanoplatforms, yet their long-term fate and cellular processing in the body is poorly understood. Here we examined the role of an endogenous iron storage protein – namely the ferritin – in the remediation of biodegradable cobalt ferrite magnetic nanoparticles. Structural and elemental analysis of ferritins close to exogenous nanoparticles within spleens and livers of mice injected in vivo with cobalt ferrite nanoparticles, suggests the intracellular transfer of degradation-derived cobalt and iron, entrapped within endogenous protein cages. In addition, the capacity of ferritin cages to accommodate and store the degradation products of cobalt ferrite nanoparticles was investigated in vitro in the acidic environment mimicking the physiological conditions that are present within the lysosomes. The magnetic, colloidal and structural follow-up of nanoparticles and proteins in the lysosome-like medium confirmed the efficient remediation of nanoparticle-released cobalt and iron ions by ferritins in solution. Metal transfer into ferritins could represent a quintessential process in which biomolecules and homeostasis regulate the local degradation of nanoparticles and recycle their by-products.

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“…Cobalt ferrite NPs induced cytotoxicity and genotoxicity in green photosynthetic algae, pigs, and humans, etc. (Kapilevich et al, ; Azaria et al, ; Barhoumi and Dewez, 2013; Ahmad et al ). Cobalt ferrite (CoFe 2 O 4 ) NPs perturbs cell cycle, promotes uncontrolled proliferation of cells, respiratory disorder (Kapilevich et al, ) by dismantling the immune system (Colognato et al, ; Hwang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Zebrafish is an excellent vertebrate model animal has long been used in research because of extraordinary resemblances with the human genome (up to 70%), well characterized development stages, transparent low cost embryos with high fecundity (Kane et al, ; Hill et al, ; Fako and Furgeson, ) and fully developed thyroid gland after 7dpf (Wang et al, ). That is why, recently zebrafish have been used extensively in toxicity and risk assessment of nanomaterials (Ahmad et al, ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of thyroid endocrine system impairment and oxidative stress mediated by cobalt ferrite (CoFe₂O₄) nanoparticles in zebrafish larvae

et al. 2015

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Fascinating super paramagnetic uniqueness of iron oxide particles at nano-scale level make them extremely useful in the state of the art therapies, equipments, and techniques. Cobalt ferrite (CoFe O ) magnetic nanoparticles (MNPs) are extensively used in nano-based medicine and electronics, results in extensive discharge and accumulation into the environment. However, very limited information is available for their endocrine disrupting potential in aquatic organisms. In this study, the thyroid endocrine disrupting ability of CoFe O NPs in Zebrafish larvae for 168-h post fertilization (hpf) was evaluated. The results showed the elevated amounts of T4 and T3 hormones by malformation of hypothalamus pituitary axis in zebrafish larvae. These elevated levels of whole body THs leads to delayed hatching, head and eye malformation, arrested development, and alterations in metabolism. The influence of THs disruption on ROS production and change in activities of catalase (CAT), mu-glutathione s-transferase (mu-GST), and acid phosphatase (AP) were also studied. The production of significantly higher amounts of in vivo generation of ROS leads to membrane damage and oxidative stress. Presences of NPs and NPs agglomerates/aggregates were also the contributing factors in mechanical damaging the membranes and physiological structure of thyroid axis. The increased activities of CAT, mu-GST, and AP confirmed the increased oxidative stress, possible DNA, and metabolic alterations, respectively. The excessive production of in vivo ROS leads to severe apoptosis in head, eye, and heart region confirming that malformation leads to malfunctioning of hypothalamus pituitary axis. ROS-induced oxidative DNA damage by formation of 8-OHdG DNA adducts elaborates the genotoxicity potential of CoFe O NPs. This study will help us to better understand the risk and assessment of endocrine disrupting potential of nanoparticles. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 2068-2080, 2016.

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Toxicity of cobalt ferrite (CoFe2O4) nanobeads in Chlorella vulgaris: Interaction, adaptation and oxidative stress

Cited by 40 publications

References 46 publications

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin

Assessment of thyroid endocrine system impairment and oxidative stress mediated by cobalt ferrite (CoFe₂O₄) nanoparticles in zebrafish larvae

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Toxicity of cobalt ferrite (CoFe2O4) nanobeads in Chlorella vulgaris: Interaction, adaptation and oxidative stress

Cited by 40 publications

References 46 publications

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

<i>In Vitro</i> Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell

Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin

Assessment of thyroid endocrine system impairment and oxidative stress mediated by cobalt ferrite (CoFe2O4) nanoparticles in zebrafish larvae

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Assessment of thyroid endocrine system impairment and oxidative stress mediated by cobalt ferrite (CoFe₂O₄) nanoparticles in zebrafish larvae