Toxicity of chronic waterborne zinc exposure in the hepatopancreas of white shrimp Litopenaeus vannamei

Liu, Zhi; Chen, Tianci; Yang, Furong; Li, Shuhong; Zhang, Shuang; Guo, Hui

doi:10.1016/j.chemosphere.2022.136553

Cited by 15 publications

(2 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The qPCR testing was performed following the manufacturer's instructions, using a CFX96 Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA) and Perfect Start Green qPCR SuperMix (TransGen). The qPCR was performed as described in a previous study by Liang et al [33]. The 2 −∆∆Ct method was used for evaluating the relative genes expression levels through the cycle thresholds (Cq), which were transformed from fluorescence information, with β-actin (GenBank accession No.…”

Section: Data Validation Via Qpcrmentioning

confidence: 99%

“…The pentose and glucuronate interconversions pathway is involved in carbohydrate metabolism [52] and significantly enriched DEGs are related to detoxification [53]. This pathway is enriched after different types of stress, such as salinity stress [53], Zn exposure [33], and exposure to phoxim and prometryne residues in shrimp [54]. These pathways were significantly changed after ammonia-N exposure, indicating that it impacted protein biosynthesis and metabolism.…”

Section: Comparison Analysis Between the Two Tissuesmentioning

confidence: 99%

See 1 more Smart Citation

Differential Toxicity Responses between Hepatopancreas and Gills in Litopenaeus vannamei under Chronic Ammonia-N Exposure

Zhang,

Liu,

Zhuo

et al. 2023

Animals

View full text Add to dashboard Cite

Ammonia nitrogen is one of the main toxic substances in aquatic cultivation environments. Chronic exposure to excessive amounts of ammonia-N creates toxic consequences, retarding the growth of aquatic organisms. This study investigated the growth performance, morphological and physiological alterations, and transcriptome changes in the hepatopancreas and gills of white shrimp Litopenaeus vannamei. The results showed that there was no significant difference in the survival rate (p > 0.05), whereas growth performance was reduced significantly in the treated groups compared to the control groups (p < 0.05). Significant structural damage and vacuolation occurred in hepatopancreas and gill tissues in the treated groups. Superoxide dismutase (SOD) activity and Na+/K+-ATPase content were significantly increased by chronic ammonia-N exposure in the two tissue groups. In addition, catalase (CAT) activity and malondialdehyde (MDA) levels were significantly altered in the hepatopancreas groups (p < 0.05), whereas no differences were observed in the gill groups (p > 0.05). There were 890 and 1572 differentially expressed genes identified in the hepatopancreas (treated versus control groups) and gills (treated versus control groups), respectively, of L. vannamei under chronic ammonia-N exposure. Functional enrichment analysis revealed associations with oxidative stress, protein synthesis, lipid metabolism, and different serine proteases. The gills maintained cellular homeostasis mainly through high expression of cytoskeleton and transcription genes, whereas the hepatopancreas down-regulated related genes in the ribosome, proteasome, and spliceosome pathways. These genes and pathways are important in the biosynthesis and transformation of living organisms. In addition, both tissues maintained organismal growth primarily through lipid metabolism, which may serve as an effective strategy for ammonia-N resistance in L. vannamei. These results provided a new perspective in understanding the mechanisms of ammonia-N resistance in crustaceans.

show abstract

Section: Data Validation Via Qpcrmentioning

confidence: 99%