Toxicity of CdTe Quantum Dots in Bacterial Strains

Dumas, Eve-Marie; Ozenne, V.; Mielke, Randall E.; Nadeau, Jay L.

doi:10.1109/tnb.2009.2017313

Cited by 55 publications

(48 citation statements)

References 36 publications

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“…Reactive oxygen species generation has been implicated in the toxic response of a number of biological systems to nanoparticles (11,29). The presence of superoxide was examined using an XTT assay, which yields a colorimetric signal when XTT is reduced (27) to antioxidant and protective responses (43) has been described for CeO 2 nanoparticles in different systems.…”

Section: Resultsmentioning

confidence: 99%

“…Superoxide production upon exposure of bacterial suspensions to various concentrations of CeO 2 nanoparticles was monitored by following the absorbance at 470 nm due to the reduction of 100 M 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) to XTT-formazan by superoxide (O 2 Ϫ ) (11,29). (vi) Microarray hybridization and analysis.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Pelletier

Suresh

Holton

et al. 2010

Appl Environ Microbiol

342

213

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Interest in engineered nanostructures has risen in recent years due to their use in energy conservation strategies and biomedicine. To ensure prudent development and use of nanomaterials, the fate and effects of such engineered structures on the environment should be understood. Interactions of nanomaterials with environmental microorganisms are inevitable, but the general consequences of such interactions remain unclear, due to a lack of standard methods for assessing such interactions. Therefore, we have initiated a multianalytical approach to understand the interactions of synthesized nanoparticles with bacterial systems. These efforts are focused initially on cerium oxide nanoparticles and model bacteria in order to evaluate characterization procedures and the possible fate of such materials in the environment. The growth and viability of the Gram-negative species Escherichia coli and Shewanella oneidensis, a metal-reducing bacterium, and the Gram-positive species Bacillus subtilis were examined relative to cerium oxide particle size, growth media, pH, and dosage. A hydrothermal synthesis approach was used to prepare cerium oxide nanoparticles of defined sizes in order to eliminate complications originating from the use of organic solvents and surfactants. Bactericidal effects were determined from MIC and CFU measurements, disk diffusion tests, and live/dead assays. For E. coli and B. subtilis, clear strain-and size-dependent inhibition was observed, whereas S. oneidensis appeared to be unaffected by the particles. Transmission electron microscopy along with microarray-based transcriptional profiling was used to understand the response mechanism of the bacteria. Use of multiple analytical approaches adds confidence to toxicity assessments, while the use of different bacterial systems highlights the potential wide-ranging effects of nanomaterial interactions in the environment.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Pelletier

Suresh

Holton

et al. 2010

Appl Environ Microbiol

342

213

View full text Add to dashboard Cite

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“…6 a, b The histogram (size distribution) of silver nanoparticles (dynamic light scattering) and zeta potential (4.8 mV) of Zn nanoparticles synthesized using Boswellia ovalifoliolata stem bark extract et al 2006). The mechanism by which the nanoparticles are able to penetrate the bacteria is not understood completely, but studies suggest that when bacteria were treated with zinc nanoparticles, changes took place in its membrane morphology that produced a significant increase in its permeability affecting proper transport through the plasma membrane (Auffan et al 2009;Brayner et al 2006) incapable of properly regulating transport through the plasma membrane, resulting into cell death (Dumas et al 2009). It is observed that zinc nanoparticles have penetrated inside the bacteria and have caused damage by interacting with phosphorus-and sulfur-containing compounds such as DNA (He et al 2008;Kim et al 2003;Kirchner et al 2005;Bayandori et al 2008).…”

Section: Antimicrobial Efficacy Of Boswellia Ovalifoliolata Stem Barkmentioning

confidence: 99%

Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles

et al. 2015

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Synthesis of metal nanoparticles using biological systems is an expanding research area in nanotechnology. Moreover, search for new nanoscale antimicrobials is been always attractive as they find numerous avenues for application in medicine. Biosynthesis of metallic nanoparticles is cost effective and eco-friendly compared to those of conventional methods of nanoparticles synthesis. Herein, we present the synthesis of zinc oxide nanoparticles using the stem bark extract of Boswellia ovalifoliolata, and evaluation of their antimicrobial efficacy. Stable ZnO nanoparticles were formed by treating 90 ml of 1 mM zinc nitrate aqueous solution with 10 ml of 10 % bark extract. The formation of B. ovalifoliolata barkextract-mediated zinc oxide nanoparticles (BZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 230 nm. Fourier transform infrared spectroscopic (FT-IR) analysis revealed that primary and secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the BZnNPs. The morphology and crystalline phase of the nanocrystals were determined by Transmission electron microscopy (TEM). The hydrodynamic diameter (20.3 nm) and a positive zeta potential (4.8 mV) were measured using the dynamic light scattering technique. The antimicrobial activity of BZnNPs was evaluated (in vitro) against fungi, Gram-negative, and Gram-positive bacteria using disk diffusion method which were isolated from the scales formed in drinking water PVC pipelines.

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“…Many manufactured nanomaterials (10), including TiO 2 (11) and either cadmium telluride (CdTe) (12) or cadmium selenide (CdSe) (13) quantum dots (QDs), have been shown to impact bacterial cell membranes and interfere with bacterial population growth (12,13). In experiments with planktonic Pseudomonas aeruginosa growing in the presence of CdSe QDs, cell membranes were disrupted by reactive oxygen species, resulting in QDs entering cells (13)(14)(15).…”

mentioning

confidence: 99%

Differential Growth of and Nanoscale TiO ₂ Accumulation in Tetrahymena thermophila by Direct Feeding versus Trophic Transfer from Pseudomonas aeruginosa

Mielke

Priester

Werlin

et al. 2013

Appl Environ Microbiol

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dNanoscale titanium dioxide (TiO 2 ) is increasingly used in consumer goods and is entering waste streams, thereby exposing and potentially affecting environmental microbes. Protozoans could either take up TiO 2 directly from water and sediments or acquire TiO 2 during bactivory (ingestion of bacteria) of TiO 2 -encrusted bacteria. Here, the route of exposure of the ciliated protozoan Tetrahymena thermophila to TiO 2 was varied and the growth of, and uptake and accumulation of TiO 2 by, T. thermophila were measured. While TiO 2 did not affect T. thermophila swimming or cellular morphology, direct TiO 2 exposure in rich growth medium resulted in a lower population yield. When TiO 2 exposure was by bactivory of Pseudomonas aeruginosa, the T. thermophila population yield and growth rate were lower than those that occurred during the bactivory of non-TiO 2 -encrusted bacteria. Regardless of the feeding mode, T. thermophila cells internalized TiO 2 into their food vacuoles. Biomagnification of TiO 2 was not observed; this was attributed to the observation that TiO 2 appeared to be unable to cross the food vacuole membrane and enter the cytoplasm. Nevertheless, our findings imply that TiO 2 could be transferred into higher trophic levels within food webs and that the food web could be affected by the decreased growth rate and yield of organisms near the base of the web.

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Toxicity of CdTe Quantum Dots in Bacterial Strains

Cited by 55 publications

References 36 publications

Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles

Differential Growth of and Nanoscale TiO ₂ Accumulation in Tetrahymena thermophila by Direct Feeding versus Trophic Transfer from Pseudomonas aeruginosa

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Toxicity of CdTe Quantum Dots in Bacterial Strains

Cited by 55 publications

References 36 publications

Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles

Differential Growth of and Nanoscale TiO 2 Accumulation in Tetrahymena thermophila by Direct Feeding versus Trophic Transfer from Pseudomonas aeruginosa

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Differential Growth of and Nanoscale TiO ₂ Accumulation in Tetrahymena thermophila by Direct Feeding versus Trophic Transfer from Pseudomonas aeruginosa