Toxicity of Ammonia Nitrogen to Ciliated Protozoa Stentor coeruleus and Coleps hirtus Isolated from Activated Sludge of Wastewater Treatment Plants

Klimek, Beata; Fyda, Janusz; Pajdak‐Stós, Agnieszka; Kocerba, Wioleta; Fiałkowska, Edyta; Sobczyk, Mateusz

doi:10.1007/s00128-012-0816-3

Cited by 17 publications

(8 citation statements)

References 22 publications

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“…The present study also showed that motile, dispersive forms of Vorticella infusionum (teletrochs) were significantly associated with increased concentrations of ammonium nitrogen in effluent from the GSBR (Pearson correlation, r ¼ 0.8168) (Figure 2b). It is known that protozoan species vary in their susceptibility to ammonium nitrogen, (Klimek et al, 2012) and acclimation may make protozoa more tolerant to the toxicity of free ammonia (Puigagut et al, 2005). No data are available in the literature on the tolerance of Pseudocohnilembus pusillus to high ammonium concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Functional groups, species, or lower rank taxons can be used as biological indicators and assist in the evaluation of conditions inside the aeration tank or the performance of the system itself (dos Santos et al, 2014). There are reports in the literature which describe the relationships between the presence of different microfauna taxons in reactors and the presence of toxic compounds in wastewater, such as chlorine (Salvad´o et al, 2000), salt (Salvad´o et al, 2001), ferric chloride (de Gregorio et al, 2010), ammonium (Klimek et al, 2012;Puigagut et al, 2005), phenol (Papadimitriou et al, 2013), and heavy metals (Nicolau et al, 2005). Other investigations related nitrification efficiency (P´erez-Uz et al, 2010;Poole, 1984), effluent quality (Puigagut et al, 2009;Zhou et al, 2006), organic loading rate (Salvad´o and Gracia, 1993), sludge retention time (Salvad´o, 1994), dissolved oxygen (dos Santos et al, 2014;Esteban et al, 1991), and sludge volume index (Zhou et al, 2006;Zhou et al, 2008) with microfauna involved in the treatment process.…”

Section: Introductionmentioning

confidence: 99%

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Impact of High‐Nitrogen Leachate on Microfauna of Aerobic Granular Sludge

Drzewicki¹,

Cydzik‐Kwiatkowska²,

Mieczkowski³

2017

Water Environment Research

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The microfauna communities present in aerobic granular sludge reactors (e.g., granule sludge batch reactor or GSBR) that are used to treat landfill leachate were studied to determine which taxons are useful as process performance indicators. Communities were analyzed during four periods: a stable reactor operation period at a temperature of 29 °C, a transitional period of biomass adaptation at a temperature of 25 °C, an additional period of stable reactor operation at 25 °C, and at 25 °C after a period of lowered dissolved oxygen concentration. The results indicate that Vorticella infusionum and telotrochs were the taxons most sensitive to environmental changes in the GSBR, showing potential as biological indicators of ammonium overload and process destabilisation. Other taxons had similar abundance, despite changing environmental conditions in the bioreactor.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of High‐Nitrogen Leachate on Microfauna of Aerobic Granular Sludge

Drzewicki¹,

Cydzik‐Kwiatkowska²,

Mieczkowski³

2017

Water Environment Research

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“…The majority of studies investigating the toxic effects of ammonia on organisms living in wastewater treatment plants (WWTPs), conducted by the staff of WWTPs, use total ammonia nitrogen (NH 3 –N + NH 4 + –N) as a measure of ammonia concentration, without paying much attention to un-ionised ammonia (NH 3 ) (Puigagut et al 2005 , 2009 ; Klimek et al 2012 ). This is somewhat surprising as it is actually NH 3 that is toxic to life whereas the effects of total ammonia are much less profound (Hellawell 1989 ).…”

Section: Resultsmentioning

confidence: 99%

Tolerance to Ammonia of Thulinius ruffoi (Bertolani, 1981), a Tardigrade Isolated from a Sewage Treatment Plant

Sobczyk

Michno

Kosztyła

et al. 2015

Bull Environ Contam Toxicol

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The acute toxicity of ammonia on Thulinius ruffoi (Bertolani, 1981), a eutardigrade isolated from a small waste water treatment plant (WWTP) in Poland, was estimated. Our results show that no active individuals survived a 24 h exposure to solutions equal to or higher than 125 mg/L of total ammonia nitrogen (NH3–N + NH4+–N), which, under the conditions in our experiment, was equivalent to 1.17 mg/L of un-ionised ammonia (NH3). The LC50 concentration of total ammonia nitrogen was equal to 52 mg/L (or 0.65 mg/L un-ionised ammonia). Given that the norms for the concentration of ammonia in treated waters leaving WWTPs are usually several times lower than the LC50 for T. ruffoi, this species does not seem to be a good bioindicator candidate for WWTPs. In this paper we also note that various ecotoxicological studies use different methodological approaches and we suggest that a more uniform methodology may aid interspecific comparisons of LC50 values.

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“…However, the Arcobacter and Acinetobacter responsible for the two sweeps observed in the AB liquor were two of the three liquor core OTUs. Furthermore, the succession occurring after the second sweep was composed of a floc core Coleps ciliate (Euk15) an omnivorous protist thriving in high ammonium (Klimek et al ., ) that possibly preyed upon the Acinetobacter, and of a BB Thiothrix OTU (Bac4022) present in 70% of the AB liquor samples >0.1% (Fig. ).…”

Section: Discussionmentioning

confidence: 99%

Bacteria and microeukaryotes are differentially segregated in sympatric wastewater microhabitats

Cohen

Pasternak

Johnke

et al. 2019

Environmental Microbiology

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Summary Wastewater purification is mostly performed in activated sludge reactors by bacterial and microeukaryotic communities, populating organic flocs and a watery liquor. While there are numerous molecular community studies of the bacterial fraction, those on microeukaryotes are rare. We performed a year‐long parallel 16S rRNA gene and 18S rRNA‐gene based analysis of the bacterial and of the microeukaryote communities, respectively, of physically separated flocs and particle‐free liquor samples from three WWTPs. This uncovered a hitherto unknown large diversity of microeukaryotes largely composed of potential phagotrophs preferentially feeding on either bacteria or other microeukaryotes. We further explored whether colonization of the microhabitats was selective, showing that for both microbial communities, different but often closely taxonomically and functionally related populations exhibiting different dynamic patterns populated the microhabitats. An analysis of their between plants‐shared core populations showed the microeukaryotes to be dispersal limited in comparison to bacteria. Finally, a detailed analysis of a weather‐caused operational disruption in one of the plants suggested that the absence of populations common to the floc and liquor habitat may negatively affect resilience and stability.

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Toxicity of Ammonia Nitrogen to Ciliated Protozoa Stentor coeruleus and Coleps hirtus Isolated from Activated Sludge of Wastewater Treatment Plants

Cited by 17 publications

References 22 publications

Impact of High‐Nitrogen Leachate on Microfauna of Aerobic Granular Sludge

Impact of High‐Nitrogen Leachate on Microfauna of Aerobic Granular Sludge

Tolerance to Ammonia of Thulinius ruffoi (Bertolani, 1981), a Tardigrade Isolated from a Sewage Treatment Plant

Bacteria and microeukaryotes are differentially segregated in sympatric wastewater microhabitats

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