Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System

Sukarjati, Sukarjati; Amilah, Susie; Sudjarwo, Sudjarwo

doi:10.20473/fmi.v54i2.8866

Cited by 3 publications

(2 citation statements)

References 17 publications

(19 reference statements)

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“…[37] PIP is a potential source of natural antioxidants that can control oxidative stress by inhibiting alpha-amylase and alphaglucosidase. [38] Several studies have shown that PIP obtained from long and black pepper has significant biological activities such as antioxidant, [39] anti-inflammatory, analgesic, [40] antihypertensive, [41] antitumor, [24] antimicrobial, [42] and antibacterial. [43] Selvendiran modulating lipid peroxidation and strengthening the antioxidant defense system.…”

Section: Discussionmentioning

confidence: 99%

Piperine mitigates oxidative stress, inflammation, and apoptosis in the testicular damage induced by cyclophosphamide in mice

Fani,

Hosseinimehr,

Zargari

et al. 2024

J Biochem & Molecular Tox

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Although cyclophosphamide (CP) has been approved as an anticancer drug, its toxic effect on most organs, especially the testis, has been established. Piperine (PIP) is an alkaloid that has antioxidant, antiapoptotic, and anti‐inflammatory activities. This study was investigated the protective effects of PIP on CP‐induced testicular toxicity in the mice. In this experimental study, 48 adult male BALB/c mice (30−35 g) were divided into six groups (n = 8), receiving normal saline (C), 5 mg/kg of PIP (PIP5), 10 mg/kg of PIP (PIP10), 200 mg/kg of CP, 200 mg/kg of CP + PIP5, and 200 mg/kg of CP + PIP10. On the eighth day of the study, blood and testis samples were prepared for serum testosterone hormone quantification, sperm analysis, histological, and immunohistochemical assays. The results of this study showed that CP induced testicular toxicity with the decrease of sperm count, motility, and viability. Also, CP treatment caused histological structure alterations in the testis, including exfoliation, degeneration, vacuolation of spermatogenic cells, and reducing the thickness of the epithelium and the diameter of the seminiferous tubule. In addition, CP decreased glutathione (GSH) levels, increased malondialdehyde (MDA) levels, Caspase‐3, and NF‐κB. At the same time, PIP treatment reduced testicular histopathological abnormalities, oxidative stress, and apoptosis that were induced by CP. These results showed that PIP improved CP‐induced testicular toxicity in mice, which can be related to its antioxidant, antiapoptotic, and anti‐inflammatory activities.

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Section: Discussionmentioning

confidence: 99%

Piperine mitigates oxidative stress, inflammation, and apoptosis in the testicular damage induced by cyclophosphamide in mice

Fani,

Hosseinimehr,

Zargari

et al. 2024

J Biochem & Molecular Tox

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“…This study's working procedure consisted of identifying the K. pneumoniae 65.5 kDa pili protein using SDS-PAGE electrophoresis based on the Laemmli method (Blancher & Jones, 2003;Brunelle & Green, 2014;Laemmli, 1970;SCHÄGGER, 2003). After identification of the desired protein band, propagation and purification are then carried out and measured using the Kingsley method (Kingsley, 1939;Sukarjati et al, 2018). The purified protein was induced intraperitoneally in group 2 mice three times with an interval of 14 days.…”

Section: Designmentioning

confidence: 99%

Increased levels of IL-10 in the spleen after induction of pili protein 65.5 kDa Klebsiella pneumoniae

Agustina,

Diana Chusna Mufida,

Enny Suswati

et al. 2023

Pharm Educ

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Background: One of the gram-negative bacteria often found as the cause of nosocomial infections is Klebsiella pneumoniae. This bacterium has a mortality rate of 28.3% due to ESBL strains that cause resistance to several antibiotics and the absence of a vaccine as a preventive measure. Objective: To determine the level of IL-10 in the spleen after induction of the protein pili K. pneumoniae 65.5 kDa in mice. Method: This study used mice spleen samples from 21 mice aged six to eight weeks in an experimental investigation with a randomised posttest-only control group design. There are three groups, K1 as a PBS-given control, 65.5 kDa antigen protein pills + Freund’s adjuvants are given to K2, and Freund's adjuvants are given to K3. Interleukin-10 concentrations were measured using the ELISA and analysed by a one-way ANOVA assay. Result: The research showed significant differences in each group at IL-10 levels after administration of the pili protein K. pneumoniae using one-way ANOVA (p = 0.036). The treatment group had the highest average levels of IL-10, so there was an increased association between exposure to pili protein and IL-10 levels in rats. A significant increase in IL-10 levels was found in the treatment group compared to the control and adjuvant groups. Conclusion: Induction of protein pili Klebsiella pneumoniae 65.5 kDa increases IL-10 levels in spleen mice.

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Bacteriospermia – A formidable player in male subfertility

et al. 2022

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Bacterial colonization of male reproductive tissues, cells, and fluids, and the subsequent impact of bacteria on the sperm architecture, activity, and fertilizing potential, has recently gained increased attention from the medical and scientific community. Current evidence strongly emphasizes the fact that the presence of bacteria in semen may have dire consequences on the resulting male fertility. Nevertheless, the molecular basis underlying bacteriospermia-associated suboptimal semen quality is sophisticated, multifactorial, and still needs further understanding. Bacterial adhesion and subsequent sperm agglutination and immobilization represent the most direct pathway of sperm–bacterial interactions. Furthermore, the release of bacterial toxins and leukocytic infiltration, associated with a massive outburst of reactive oxygen species, have been repeatedly associated with sperm dysfunction in bacteria-infested semen. This review serves as a summary of the present knowledge on bacteriospermia-associated male subfertility. Furthermore, we strived to outline the currently available methods for assessing bacterial profiles in semen and to outline the most promising strategies for the prevention and/or management of bacteriospermia in practice.

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Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System

Cited by 3 publications

References 17 publications

Piperine mitigates oxidative stress, inflammation, and apoptosis in the testicular damage induced by cyclophosphamide in mice

Piperine mitigates oxidative stress, inflammation, and apoptosis in the testicular damage induced by cyclophosphamide in mice

Increased levels of IL-10 in the spleen after induction of pili protein 65.5 kDa Klebsiella pneumoniae

Bacteriospermia – A formidable player in male subfertility

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