Toxicity in different molecular-weight fractions of sludge treating synthetic wastewater containing 4-chlorophenol

Zhao, Jian; Chen, Xiurong; Zhao, Jun; Lin, Fengkai; Zheng, Bo; He, Ya‐Ling; Wang, Lu; Shi, Zhendong

doi:10.1016/j.ibiod.2015.06.005

Cited by 21 publications

(13 citation statements)

References 39 publications

(47 reference statements)

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“…P. phosphoreum was successfully used for determination of different molecular weight fractions of sludge treating synthetic wastewater containing 4-chlorophenol (Zhao et al 2015). V. fischeri was used for assessment of ecotoxicity of mobile forms of heavy metals in sewage sludge (Gondek et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Southern Germany

Сазыкин

Сазыкина

Khmelevtsova

et al. 2016

Int. J. Environ. Sci. Technol.

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To assess the ecotoxicological and sanitary situation in two European metropolis, Rostov-on-Don (Southern Russia) and Munich (Southern Germany), wastewaters of the two cities were examined with a panel of bacterial lux-biosensors:The presence of different genotoxic compounds and substances with the oxidative and membrane-damaging effects was revealed in contaminated wastewater with the applied panel of the lux-biosensors. The integral toxicity was approximately the same in both cities but demonstrated opposite trends. The presence of genotoxicants and peroxides was higher in the majority of the Munich wastewater samples. There were also differences in the presence of individual toxicants. The presence of the genotoxic compounds might also promote development and dissemination of several antibiotic resistance traits found in microorganisms, a feature more pronounced in Rostov-on-Don wastewaters. By means of polymerase chain reaction assay, antibiotic resistance genes to such antibiotics as ermB, vim and vanB were revealed in two Munich samples. Antibiotic resistance genes were present at all Rostov samples, and genes ndm, vanA, vanB and ermB were found. Taken together, the proposed analytical approach with the application of the constructed panel of biosensors can be applied for monitoring of the ecotoxicological contamination in the wastewaters of large cities.

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Section: Resultsmentioning

confidence: 99%

Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Southern Germany

Сазыкин

Сазыкина

Khmelevtsova

et al. 2016

Int. J. Environ. Sci. Technol.

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“…The molecular-weight distribution of the organics in the sludge extract was measured by ultrafiltration with different bore diameter membranes (10 000, 5000 and 500 Da) according to Zhao's method. 16 Vibrio qinghaiensis sp Q67 (obtained from Shanghai Anlikang Co., ltd., China) was used to characterize the ecotoxicity of the sludge extract in the biological toxicity test instrument (DXY-2; Institute of Soil Science, Chinese Academy of Sciences, China) according to Ma's method. 25 The toxicity (TOX) was quantified by the luminescence inhibition rate calculated by Eqn (1):…”

Section: Liquid Phasementioning

confidence: 99%

“…10,13 During the process of industrial wastewater treatment, sludge biotoxicity results and accumulates, resulting from the degradation of refractory or toxic organic compounds 14,15 , with molecular weights ranging from 500 Da to>10 000 Da. 16 Therefore, the effects of the above toxicity on the growth of microalgae should be considered when considering sludge extract from industrial wastewater. 10,17 Chlorella vulgaris has been widely studied for its rapid growth rate, efficient utilization of solar energy 18,19 and strong adaptability to polluted environments (e.g.…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Chen

Zhou

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The cultivation of Chlorella vulgaris present in toxic sludge extract from resorcinol‐rich wastewater was investigated to simultaneously determine the biomass production and toxicity removal. RESULTS A comparison of the blank and the toxic sludge extract revealed that the sludge toxicity originating from the degradation of resorcinol was highly correlated to the mass fraction at 5000–10000 Da (R = 0.987, P = 0.013) and led to a decline in biomass (1.49 g L−1 to 1.36 g L−1 in 30 days) along with a reduced utilization rate of organics in the sludge extract, especially in the early stage (0–5 days) of culture. The effects of sludge toxicity were characterized by the downregulation of proteins associated with carbon (C) metabolism and the upregulation of proteins involved in photoreaction by proteomics. CONCLUSION The sludge toxicity directly caused a decline in biomass production of C. vulgaris resulting from the downregulation of C metabolism. However, the promotion of biomass for heterotrophism was more significant than the inhibitory effect in the toxic sludge extract when compared with BG11 medium. © 2018 Society of Chemical Industry

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“…In the process of 4‐CP wastewater treatment, our previous studies have explored the correlation between different molecular‐weight organics in sludge and sludge toxicity in single SBR cycle, the contribution of secretions from microbes on sludge toxicity, the influence of elevated influent 4‐CP loads on main components of polysaccharides and proteins and sludge toxicity, the correlation between the components of polysaccharides and proteins and sludge toxicity and the proteins’ expression in sludge with or without 4‐CP using two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) technology (Zhao et al, ; Zhao et al, ; Zhao et al, ). And in this study, on the basis of previous studies, 4‐CP wastewater was sequentially treated using activated sludge process in a SBR with HRT of 8 hours.…”

Section: Introductionmentioning

confidence: 99%

“…And in this study, on the basis of previous studies, 4‐CP wastewater was sequentially treated using activated sludge process in a SBR with HRT of 8 hours. The dynamic change of sludge toxicity was evaluated, and contrasted to a previous study of HRT 12 hours (Zhao et al, ; Zhao et al, ). After the activated sludge was effectively acclimated with 4‐CP, the main proteins in the sludge were analysed and identified via SDS‐PAGE plus MS, and their influence on 4‐CP biodegradation and sludge toxicity was analysed, thus providing more information on the stable operation strategy of industrial wastewater treatment process.…”

Section: Introductionmentioning

confidence: 99%

Effects of hydraulic retention time and proteins on sludge toxicity for 4‐chlorophenol wastewater treatment in sequencing batch reactors

Zhao

Chen

et al. 2018

Water & Environment J

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Due to the higher uncertainty of environmental risk for pollutants’ treatment by activated sludge, 10 mg/L influent 4‐chlorophenol (4‐CP) treated via a sequencing batch reactor (SBR) was used as acclimated SBR. Another SBR was used as control SBR without 4‐CP. The effects of hydraulic retention time (HRT) and proteins on sludge toxicity for 4‐CP treatment were analysed, and compared to the control SBR. Results showed that the sludge toxicity in acclimated SBR was significantly higher than that of the control SBR. Shortening HRT from 12 to 8 hours was beneficial to degrade 4‐CP and lower sludge toxicity. The identified highly expressed protein of ABC transporter co‐existed in control and acclimated SBRs, while other proteins of TonB‐dependent receptor, heat shock 70 kDa protein and superoxide dismutase in acclimated sludge were overexpressed relative to the control sludge, which played an important function in degrading 4‐CP, resisting 4‐CP toxicity and eliminating sludge toxicity.

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Toxicity in different molecular-weight fractions of sludge treating synthetic wastewater containing 4-chlorophenol

Cited by 21 publications

References 39 publications

Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Southern Germany

Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Southern Germany

Cultivation of Chlorella vulgaris in sludge extract from resorcinol‐rich wastewater: the removal and inhibitory effect of sludge toxicity

Effects of hydraulic retention time and proteins on sludge toxicity for 4‐chlorophenol wastewater treatment in sequencing batch reactors

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