Toxicity impact of fenvalerate on the gill tissue of Oreochromis mossambicus with respect to biochemical changes utilizing FTIR and principal component analysis

Velmurugan, B.; Senthilkumaar, P.; Karthikeyan, S.

doi:10.1007/s10867-018-9484-9

Cited by 22 publications

(7 citation statements)

References 43 publications

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“…The further antiparallel β sheet was formed and an increase in band area 9-12% was observed with respect to control. This change in the secondary structure is in agreement with our earlier studies using FTIR on fish tissues [17]. Similar results of a decrease in the protein profile exposed to effluent toxicity of Channa punctatus (Bloch) were reported by Maruthi and Subba Rao [43].…”

Section: Ftir Self Deconvolution Deduced By Curve Fitting Analysis In the Amide I Regionsupporting

confidence: 92%

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Spectral profile index changes as biomarker of toxicity in Catla catla (Hamilton, 1822) edible fish studied using FTIR and principle component analysis

Kumar¹,

Kumari²,

Kavitha

et al. 2020

SN Appl. Sci.

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The study was carried out to evaluate the toxicity in gill tissue of edible fish Catla catla using FTIR spectra. Fourier selfdeconvolution obtained by curve fitting was applied in the lipid (3000-2800 cm −1 ), carbohydrates (1000-1100 cm −1 ) and in the amide region (1700-1600 cm −1 ). These spectral changes were used as biochemical parameters to assess the degree of toxicity. A disorder in lipid changes was measured by frequency shift and intensity changes in the CH 2 asymmetric stretching band. This change in the fatty acid composition in fish could be used as biomarkers of toxic effect. Decreases in lactic acid (6-16%) clarify the lipid peroxidation which is the primary mechanism of toxicity. The deconvolution in the amide region shows peaks at 1621 cm −1 , 1637 cm −1 due to β sheet; 1652 cm −1 and 1667 cm −1 due to α helix and 1683 cm −1 due to antiparallel β sheet. The results show a decrease by (3-7%) in α helix and increase by (13-40%) in β sheet structure. This shows β sheet formation of protein secondary structure due to toxicity. PCA plots indicate protein and lipids have strong positive loadings. The study shows the spectral variation is considered as an ideal biomarker with a high degree of accuracy of test organism to examine the toxicity of pollutants.

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Section: Ftir Self Deconvolution Deduced By Curve Fitting Analysis In the Amide I Regionsupporting

confidence: 92%

“…The bands rise at ~ 3013 cm −1 which indicates the presence of HC=CH group olefinic molecules. This band is used as a varying measure of degrees of unsaturation of phospholipids [17]. Lipids give rise to a number of absorption in FTIR spectra.…”

Section: Resultsmentioning

confidence: 99%

Spectral profile index changes as biomarker of toxicity in Catla catla (Hamilton, 1822) edible fish studied using FTIR and principle component analysis

Kumar¹,

Kumari²,

Kavitha

et al. 2020

SN Appl. Sci.

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“…The results revealed that toxicity due to tannery effluent influences metabolic pathways of Oreochromis mossambicus and the biochemical studies utilizing FTIR can fill in as incredible biomarkers of tannery effluent contamination. Similar results were observed in Cirrhinus mrigala and Oreochromis mossambicus treated with heavy metals and pyrethroids respectively (Karthikeyan et al, 2012;Velmurugan et al, 2018). It is observed from that decrease in muscle fat was observed due to tannery treatment and the increase in fat noted after supplementation with feed treatment.…”

Section: Discussionsupporting

confidence: 74%

Ameliorative effect of Nelumbo nucifera seeds and wheat grass formulated feed against tannery effluent toxicity and stress in freshwater fish Oreochromis mossambicus

Sankar¹,

Kumaran²,

Saravanan³

2022

EEC

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Amelioration of tannery effluent toxicity and stress revival was analysed through an approach by preparing a formulated feed with feed components comprising Nelumbo nucifera seeds and Wheatgrass as major ingredients in an enhanced formulation. The formulated feed was fed to fingerlings of Oreochromis mossambicus exposed to tannery effluent in laboratory confinement to observe its effect on growth and protective effect from toxic nature of the effluent. This study aimed to assess the effect of the sublethal concentration of tannery effluent over an exposure period of 45 days. The LC50was analysed for the tannery effluent to select the suitable sublethal concentration. The body weight differences was be determined, the muscle tissue was analysed for biomolecular constituents by Fourier Transform Infrared spectroscopy (FT-IR) analysis. Alterations were observed in body weight and muscle tissue biochemical constituents in fishes exposed to the effluent fed on normal diet and formulated feed.

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“…Among different techniques, Fourier transform-infrared (FTIR) spectroscopy, a form of vibrational spectroscopy, is one common analytical platform used in metabolic fingerprinting. It has been successfully applied to differentiate functional biochemical groups in the liver of fish exposed to different rearing conditions 11 , 12 , to determine the changes caused by spoilage in gilthead seabream 13 and salmon 14 , and to identify alterations provoked by toxic chemicals, in rainbow trout 15 , 16 , rohu 17 , 18 , Mozambique tilapia 19 and catfish 20 . Furthermore, FTIR spectroscopy was also applied to assess cod liver oil quality 21 and characterize hake lipids and lipid changes during frozen storage 22 .…”

Section: Introductionmentioning

confidence: 99%

“…No reference has been found in the literature regarding the use of FTIR spectroscopy to investigate the metabolic alterations in farmed fish induced by everyday aquaculture production stressors. However, previous studies on the effects of stressful conditions in algae 27 , yeast 28 , bacteria 29 and fish toxicology 19 demonstrate the potential of its application in this field. One great advantage of this spectroscopic technique is its ability to provide a broad outlook on fish metabolism without any preconceptions.…”

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confidence: 99%

Mid-infrared spectroscopic screening of metabolic alterations in stress-exposed gilthead seabream (Sparus aurata)

Magalhães

Carrilho

Schrama

et al. 2020

Sci Rep

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Stress triggers a battery of physiological responses in fish, including the activation of metabolic pathways involved in energy production, which helps the animal to cope with the adverse situation. Prolonged exposure to stressful farming conditions may induce adverse effects at the whole-animal level, impairing welfare. Fourier transform infrared (FTIR) spectroscopy is a rapid biochemical fingerprinting technique, that, combined with chemometrics, was applied to disclose the metabolic alterations in the fish liver as a result of exposure to standard stressful practices in aquaculture. Gilthead seabream (Sparus aurata) adults exposed to different stressors were used as model species. Spectra were preprocessed before multivariate statistical analysis. Principal components analysis (PCA) was used for pattern recognition and identification of the most discriminatory wavenumbers. Key spectral features were selected and used for classification using the k-nearest neighbour (KNN) algorithm to evaluate whether the spectral changes allowed for the reliable discrimination between experimental groups. PCA loadings suggested that major variations in the hepatic infrared spectra responsible for the discrimination between the experimental groups were due to differences in the intensity of absorption bands associated with proteins, lipids and carbohydrates. This broad-range technique can thus be useful in an exploratory approach before any targeted analysis.

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Toxicity impact of fenvalerate on the gill tissue of Oreochromis mossambicus with respect to biochemical changes utilizing FTIR and principal component analysis

Cited by 22 publications

References 43 publications

Spectral profile index changes as biomarker of toxicity in Catla catla (Hamilton, 1822) edible fish studied using FTIR and principle component analysis

Spectral profile index changes as biomarker of toxicity in Catla catla (Hamilton, 1822) edible fish studied using FTIR and principle component analysis

Ameliorative effect of Nelumbo nucifera seeds and wheat grass formulated feed against tannery effluent toxicity and stress in freshwater fish Oreochromis mossambicus

Mid-infrared spectroscopic screening of metabolic alterations in stress-exposed gilthead seabream (Sparus aurata)

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