Toxicity Evaluation and Genetic Improvement of Bacillus thuringiensis Isolated from Different Regions in Assiut, Egypt against Mosquito Larvae

Rafeek, Abdallah; Hesham, Abd El-Latif; Abd-Ella, Aly A.; Mahmoud, Ghada Abd‐Elmonsef; Elfarash, A.E.

doi:10.22207/jpam.17.1.03

Cited by 1 publication

(1 citation statement)

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“…Then, rhizosphere stock was diluted until the suitable concentration. For total aerobic bacteria, 1 mL of the rhizosphere soil solution was passed into a sterilized Petri dish and mixed with nutrient agar medium, incubated at 30 ± 1 • C for 48 h (Heraeus B5050E Incubator, Gemini BV, Prinses Beatrixlaan 301, 7312 DG Apeldoorn, The Netherlands) [63]. For nitrogen-fixing bacteria, 1 mL of the rhizosphere soil solution was passed into a sterilized Petri dish and mixed with nitrogenfree mannitol medium, and incubated at 30 ± 1 • C for 48 h (Heraeus B5050E Incubator, Gemini BV, Prinses Beatrixlaan 301, 7312 DG Apeldoorn, The Netherlands) [58].…”

Section: Microbiological Soil Analysismentioning

confidence: 99%

Responses of Pea (Pisum sativum L.) to Single and Consortium Bio-Fertilizers in Clay and Newly Reclaimed Soils

Mahmoud,

Abeed,

Mostafa

et al. 2023

Plants

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The huge development of climatic change highly affects our crop production and soil fertility. Also, the rise in the uncontrolled, excessive use of chemical fertilizers diminishes the soil prosperity and generates pollutants, threatening all environmental life forms, including us. Replacement of these chemical fertilizers with natural ones is becoming an inevitable environmental strategy. In our study, we evaluated the responses of Pisum sativum L. to the action of single species and consortiums of plant growth-promoting bacteria (Azotobacter chroococcum, Bacillus megaterium, and Bacillus cerkularice) in clay and new reclaimed soil types in terms of phenotype, yield components, and physiological and biochemical responses. Data analysis showed single or consortium microbial inoculation significantly increased the measured traits under clay and calcareous sandy soils compared to the control. Shoot physiological and biochemical activities, and seed biochemical activities were significantly enhanced with the inoculation of pea seeds with three types of bacteria in both soil types. The bud numbers, fresh weight, and seeds’ dry weight increased in seeds treated with A. chroococcum and B. megaterium in the sandy soil. Taken together, these findings suggested that the inoculation of plants with PGP bacteria could be used to diminish the implementation of chemical fertilizer and improve the goodness of agricultural products. These findings expand the understanding of the responsive mechanism of microbial inoculation under different soil types, especially at physiological and biochemical levels.

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Section: Microbiological Soil Analysismentioning

confidence: 99%