Toxicity effects of organosulfur compounds on anaerobic microbial metabolism

Londry, Kathleen L.; Suflita, Joseph M.

doi:10.1002/etc.5620170702

Cited by 15 publications

(7 citation statements)

References 25 publications

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“…Our research showed that 50% inhibition of methanogenesis by methanethiol took place at concentrations between 7 and 10 mM for the substrates methanol, acetate, and hydrogen. This is in agreement with observations made by Londry and Suflita [2], who found that thiols inhibited methanogenesis at concentrations above 5 mM. They tested the toxicity of a mixture of long-chain (C5-C8) and cyclic thiols with lactate as the substrate.…”

Section: Toxicity Of Thiols On Methanogenesissupporting

confidence: 92%

“…In addition to inorganic sulfur species (e.g., sulfide, sulfate, sulfite, and thiosulfate), more than 200 sulfurcontaining organic compounds have been identified in crude oils. These include sulfides, thiols, thiophenes, substituted benzo-and dibenzothiophenes, and more complex molecules [2,3]. In this paper, we focus on the anaerobic degradation and toxicity of some short-chain volatile (organic) sulfur compounds that are present in wastewater streams of refineries (e.g., as a result of the extraction of liquefied petroleum gas).…”

Section: Introductionmentioning

confidence: 99%

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Volatile organic sulfur compounds in anaerobic sludge and sediments: Biodegradation and toxicity

Leerdam

Bok

Lomans

et al. 2006

Enviro Toxic and Chemistry

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A variety of environmental samples was screened for anaerobic degradation of methanethiol, ethanethiol, propanethiol, dimethylsulfide, and dimethyldisulfide. All sludge and sediment samples degraded methanethiol, dimethylsulfide, and dimethyldisulfide anaerobically. In contrast, ethanethiol and propanethiol were not degraded by the samples investigated under any of the conditions tested. Methanethiol, dimethylsulfide, and dimethyldisulfide were mainly degraded by methanogenic archaea. In the presence of sulfate and the methanogenic inhibitor bromoethane sulfonate, degradation of these compounds coupled to sulfate reduction occurred as well, but at much lower rates. Besides their biodegradability, also the toxicity of methanethiol, ethanethiol, and propanethiol to methanogenesis with methanol, acetate, and H2/CO2 as the substrates was assessed. The 50% inhibition concentration of methanethiol on the methane production from these substrates ranged between 7 and 10 mM. The 50% inhibition concentration values of ethanethiol and propanethiol for the degradation of methanol and acetate were between 6 and 8 mM, whereas hydrogen consumers were less affected by ethanethiol and propanethiol, as indicated by their higher 50% inhibition concentration (14 mM). Sulfide inhibited methanethiol degradation already at relatively low concentrations: methanethiol degradation was almost completely inhibited at an initial sulfide concentration of 8 mM. These results define the operational limits of anaerobic technologies for the treatment of volatile organic sulfur compounds in sulfide-containing wastewater streams.

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Section: Toxicity Of Thiols On Methanogenesissupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Volatile organic sulfur compounds in anaerobic sludge and sediments: Biodegradation and toxicity

Leerdam

Bok

Lomans

et al. 2006

Enviro Toxic and Chemistry

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“…Th e pH levels observed in the four treatments never dropped below approximately 7, and it therefore does not appear likely that pH alone could cause signifi cant inhibition or delay in methane production. Accumulation of volatile organic S compounds such as MT could be toxic to methanogens (Londry and Sufl ita, 1998;van Leerdam et al, 2006), but in this study MT concentrations were generally below toxic levels. Wang et al (2009) observed an inhibition of methane production at 12 mmol L −1 propionic acid, a level also reached in the present study.…”

Section: Cycling Of Volatile Sulfur Compoundsmentioning

confidence: 56%

Emissions of Sulfur‐Containing Odorants, Ammonia, and Methane from Pig Slurry: Effects of Dietary Methionine and Benzoic Acid

et al. 2010

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Supplementation of benzoic acid to pig diets reduces the pH of urine and may thereby affect emissions of ammonia and other gases from slurry, including sulfur-containing compounds that are expected to play a role in odor emission. Over a period of 112 d, we investigated hydrogen sulfide (H(2)S), methanethiol (MT), dimethyl sulfide (DMS), dimethyl disulfide (DMDS), and dimethyl trisulfide (DMTS), as well as ammonia and methane emissions from stored pig slurry. The slurry was derived from a feeding experiment with four pig diets in a factorial design with 2% (w/w) benzoic acid and 1% (w/w) methionine supplementation as treatments. Benzoic acid reduced slurry pH by 1 to 1.5 units and ammonia emissions by 60 to 70% for up to 2 mo of storage, and a considerable, but transitory reduction of methane emissions was also observed after 4 to 5 wk. All five volatile sulfur (S) compounds were identified in gas emitted from the slurry of the control treatment, which came from pigs fed according to Danish recommendations for amino acids and minerals. The emission patterns of volatile S compounds suggested an intense cycling between pools of organic S in the slurries, with urinary sulfate as the main source. Diet supplementation with methionine significantly increased all S emissions. Diet supplementation with benzoic acid reduced emissions of H(2)S and DMTS compared with the control slurry and moderately increased the concentrations of MT. Sulfur gas emissions were influenced by a strong interaction between methionine and benzoic acid treatments, which caused a significant increase in emissions of especially MT, but also of DMDS. In conclusion, addition of 2% benzoic acid to pig diets effectively reduced ammonia volatilization, but interactions with dietary S may increase odor problems.

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“…In addition to the inorganic sulfur species, such as elemental sulfur, sulfate, sulfite, thiosulfate, and sulfide, more than 200 sulfurcontaining organic compounds have been identified in crude oils. These include sulfides, thiols, thiophenes, substituted benzo-and dibenzothiophenes, and many considerably more complex molecules (Londry andSuflita, 1998, andMonticello andFinnerty, 1985).…”

Section: Introductionmentioning

confidence: 99%

Degradation of Methanethiol in a Continuously Operated Upflow Anaerobic Sludge‐Blanket Reactor

Sipma

Bree²,

Janssen³

et al. 2002

Water Environment Research

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The feasibility of anaerobic treatment of wastewater containing volatile organic sulfur compounds was investigated using biomass originating from an anaerobic wastewater treatment facility treating brewery wastewater. Interest focused mainly on the degradation of methanethiol (MT), an extremely volatile and malodorous sulfur compound. Formation of hydrogen sulfide from methanethiol, dimethyl sulfide (DMS), and dimethyl disulfide (DMDS) was observed. Batch experiments showed that methanethiol was predominantly used by methanogenic bacteria as the sole source of energy and carbon. Methane was formed on MT degradation, and in the presence of 2bromoethanesulfonic acid (BES), a specific inhibitor of methanogens, MT conversion was strongly inhibited. During the MT degradation, DMS and DMDS were the other primary compounds found. Relatively small quantities of DMS were present; whereas the DMDS concentrations could accumulate as a result of the relatively fast rate at which methanethiol autoxidizes in the presence of minor amounts of molecular oxygen. It was shown that DMS and DMDS could be biologically degraded, resulting in the formation of methane and hydrogen sulfide. In a continuous experiment using a laboratory-scale upflow anaerobic sludge-blanket (UASB) reactor with a volume of 2.0 L, the feasibility of anaerobic treatment of methanethiol was tested. The reactor was operated at a hydraulic residence time (HRT) of 6 hours, temperature of 30°C, and pH of 7.3 to 7.6. The maximal MT conversion efficiency in the continuous experiment was reached after approximately 70 days and exceeded 97% at an influent concentration of 6 mM, corresponding to a MT loading rate of 25 mM/d. The specific MT degradation rate, as determined after 40 days of operation in the UASB, measured 1.1 Ϯ 0.1 mM MT/g volatile suspended solids⅐d. These results show that anaerobic treatment of MT-containing waste streams is an interesting alternative for currently used physicochemical treatment methods. Water Environ. Res., 74, 264 (2002).

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Toxicity effects of organosulfur compounds on anaerobic microbial metabolism

Cited by 15 publications

References 25 publications

Volatile organic sulfur compounds in anaerobic sludge and sediments: Biodegradation and toxicity

Volatile organic sulfur compounds in anaerobic sludge and sediments: Biodegradation and toxicity

Emissions of Sulfur‐Containing Odorants, Ammonia, and Methane from Pig Slurry: Effects of Dietary Methionine and Benzoic Acid

Degradation of Methanethiol in a Continuously Operated Upflow Anaerobic Sludge‐Blanket Reactor

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