Toxicity, Biocompatibility, pH-Responsiveness and Methotrexate Release from PVA/Hyaluronic Acid Cryogels for Psoriasis Therapy

Cheaburu-Yılmaz, Catalina Natalia; Pamfil, Daniela; Vasile, Cornelia; Bibire, Nela; Lupusoru, Raoul-Vasile; Zamfir, Carmen-Lăcrămioara; Lupușoru, Cătălina Elena

doi:10.3390/polym9040123

Cited by 27 publications

(18 citation statements)

References 55 publications

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“…The mechanical properties of the composites were improved using this A significant increase in the white blood cell count may mean the presence of a parasite or toxin [37]. However, the control samples had a healthier blood count than did the other rats, which was probably the result of proper feeding [41].…”

Section: Discussionmentioning

confidence: 99%

“…A significant increase in the white blood cell count may mean the presence of a parasite or toxin [37]. However, the control samples had a healthier blood count than did the other rats, which was probably the result of proper feeding [41]. A change in the liver architecture under a light microscope is usually ascribed to tension in the liver caused by a parasite or toxic substance [37].…”

Section: Biotoxicity Propertiesmentioning

confidence: 99%

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The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

et al. 2020

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The current research trend for excellent miscibility in polymer mixing is the use of plasticizers. The use of most plasticizers usually has some negative effects on the mechanical properties of the resulting composite and can sometimes make it toxic, which makes such polymers unsuitable for biomedical applications. This research focuses on the improvement of the miscibility of polymer composites using two-step mixing with a rheomixer and a mix extruder. Polylactic acid (PLA), chitin, and starch were produced after two-step mixing, using a compression molding method with decreasing composition variation (between 8% to 2%) of chitin and increasing starch content. A dynamic mechanical analysis (DMA) was used to study the mechanical behavior of the composite at various temperatures. The tensile strength, yield, elastic modulus, impact, morphology, and compatibility properties were also studied. The DMA results showed a glass transition temperature range of 50 °C to 100 °C for all samples, with a distinct peak value for the loss modulus and factor. The single distinct peak value meant the polymer blend was compatible. The storage and loss modulus increased with an increase in blending, while the loss factor decreased, indicating excellent compatibility and miscibility of the composite components. The mechanical properties of the samples improved compared to neat PLA. Small voids and immiscibility were noticed in the scanning electron microscopy images, and this was corroborated by X-ray diffraction graphs that showed an improvement in the crystalline nature of PLA with starch. Bioabsorption and toxicity tests showed compatibility with the rat system, which is similar to the human system.

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Section: Discussionmentioning

confidence: 99%

Section: Biotoxicity Propertiesmentioning

confidence: 99%

The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

et al. 2020

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“…Hydrogel-forming water-soluble polymers have many functional groups, such as OH, COOH, and NH 2 . 3D network can be established by covalent bonding between these functional groups using glutaraldehyde and EDC/NHS (Balakrishnan et al, 2013 ; Omobono et al, 2015 ; Cheaburu Yilmaz et al, 2017 ). However, the toxicity of crosslinking agents and process limit the TE applications.…”

Section: Materials and Crosslinking Methods For Functional Hydrogels mentioning

confidence: 99%

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

et al. 2018

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Tissue engineering (TE) has been used as an attractive and efficient process to restore the original tissue structures and functions through the combination of biodegradable scaffolds, seeded cells, and biological factors. As a unique type of scaffolds, hydrogels have been frequently used for TE because of their similar 3D structures to the native extracellular matrix (ECM), as well as their tunable biochemical and biophysical properties to control cell functions such as cell adhesion, migration, proliferation, and differentiation. Various types of hydrogels have been prepared from naturally derived biomaterials, synthetic polymers, or their combination, showing their promise in TE. This review summarizes the very recent progress of hydrogels used for TE applications. The strategies for tuning biophysical and biochemical properties, and structures of hydrogels are first introduced. Their influences on cell functions and promotive effects on tissue regeneration are then highlighted.

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“…Ferulic acid derivatives were evaluated in vivo, determining the lethal dose 50 (LD50) on Swiss white mice [9][10][11]. The study was carried out in accordance with the current guidelines on the ethics and ethics of the study on laboratory animals (Law no.…”

Section: Determination Of Acute Toxicitymentioning

confidence: 99%

Biological Evaluation of Azetidine-2-One Derivatives of Ferulic Acid as Promising Anti-Inflammatory Agents

et al. 2020

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The purpose of this study was to evaluate the in vivo biological potential of new azetidine-2-one derivatives of ferulic acid (6a–f). First, the in vivo acute toxicity of azetidine-2-one derivatives of ferulic acid on Swiss white mice was investigated and, based on the obtained results, it can be stated that the studied derivatives belong to compounds with moderate toxicity. The in vivo anti-inflammatory potential of these derivatives was determined in a model of acute inflammation induced by carrageenan in rats and in a chronic inflammation model induced in rats using the granuloma test. In the acute inflammation model, all the studied compounds had a maximum anti-inflammatory effect 24 h after administration, which suggests that these compounds may be classified, from a pharmacokinetic point of view, in the category of long-acting compounds. The most active compound in the series was found to be compound 6b. In the case of the chronic inflammation model, it was observed that the studied compounds (6a–f) reduced the formation of granulation tissue compared to the control group, having an intense effect of inhibiting the proliferative component. The most important inhibitory effect of inhibiting the proliferative component was recorded for compound 6b. Additionally, the investigation of liver function was performed by determining the serum levels of liver enzymes aspartate transaminase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and bilirubin (total and direct). The results showed that, in the series of azetidin-2-one derivatives, the liver enzymes concentration values were close to those recorded for the reference anti-inflammatories (diclofenac sodium and indomethacin) and slightly higher compared to the values for the healthy control group. At the end of the experiment, the animals were euthanized and fragments of liver, lung, and kidney tissue were taken from all groups in the study. These were processed for histopathological examination, and we noticed no major changes in the groups treated with the azetidine 2-one derivatives of ferulic acid compared to the healthy groups.

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Toxicity, Biocompatibility, pH-Responsiveness and Methotrexate Release from PVA/Hyaluronic Acid Cryogels for Psoriasis Therapy

Cited by 27 publications

References 55 publications

The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

The Role of Two-Step Blending in the Properties of Starch/Chitin/Polylactic Acid Biodegradable Composites for Biomedical Applications

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

Biological Evaluation of Azetidine-2-One Derivatives of Ferulic Acid as Promising Anti-Inflammatory Agents

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