Toxicity assessment of pollutants sorbed on environmental microplastics collected on beaches: Part II-adverse effects on Japanese medaka early life stages

Pannetier, Pauline; Morin, Bénédicte; Clérandeau, Christelle; Laurent, Jennifer; Chapelle, Coline; Cachot, Jérôme

doi:10.1016/j.envpol.2018.10.129

Cited by 69 publications

(23 citation statements)

References 67 publications

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“…The cell-line approach would appear to be a relevant alternative tool, making it possible to study a large number of samples at different concentrations to obtain information about the toxicity of environmental samples of MPs. This type of prescreening on cell-lines could be combined with a toxicity assay on whole organisms, including fish at different life stages (Pannetier et al, 2018b). This study demonstrated the presence of organic pollutants at the surface of microplastics from different Pacific and Atlantic island, as well as demonstrating the toxicity of those pollutants to fish cells.…”

Section: Conclusion 24mentioning

confidence: 83%

“…For positive control, DMSO extract was diluted by 2500 before ethanolic solution addition. Analyses was realized by gas chromatography system coupled to an triple quadrupole mass spectrometer following same protocol and conditions described in previous studies (Lacroix et al, 2014;Pannetier et al, 2018b). Results are expressed as ng analytes.g -1 plastic.…”

Section: Quantification Of Organic Pollutants In Extractmentioning

confidence: 99%

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Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line

Pannetier

Cachot

Clérandeau

et al. 2019

Environmental Pollution

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Section: Conclusion 24mentioning

confidence: 83%

Section: Quantification Of Organic Pollutants In Extractmentioning

confidence: 99%

Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line

Pannetier

Cachot

Clérandeau

et al. 2019

Environmental Pollution

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“…Fish larvae, including zebrafish embryos, have also been studied to evaluate the potential effect of PS NPs/MPs combined with POPs. Pannetier et al [ 32 ] assessed the potential transport of B(a)P into medaka embryos, using MPs collected from beaches. A mixture with 1% of <600 µm MPs (PP, PE, and PS) coated with B(a)P (250 µg of B(a)P/g of MPs) caused high mortality (81%) of medaka embryos after 48 h of exposure, as well as an increase of 7-ethoxyresorufin-O-deethylase (EROD) activity.…”

Section: Introductionmentioning

confidence: 99%

Screening of the Toxicity of Polystyrene Nano- and Microplastics Alone and in Combination with Benzo(a)pyrene in Brine Shrimp Larvae and Zebrafish Embryos

et al. 2022

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The occurrence of nanoplastics (NPs) and microplastics (MPs) in aquatic ecosystems and their capacity to sorb hydrophobic pollutants is nowadays an issue of great concern. This study aimed to assess the potential bioavailability and acute toxicity of polystyrene (PS) NPs (50 and 500 nm) and of MPs (4.5 µm), alone and with sorbed benzo(a)pyrene (B(a)P), in the embryo/larval stages of brine shrimps and zebrafish. Exposure to pristine plastics up to 50.1 mg PS/L did not cause significant impact on brine shrimp survival, while some treatments of plastics-B(a)P and all concentrations of B(a)P (0.1–10 mg/L) resulted acutely toxic. In zebrafish, only the highest concentrations of MPs-B(a)P and B(a)P caused a significant increase of malformation prevalence. Ingestion of NPs was observed by 24–48 h of exposure in the two organisms (from 0.069 to 6.87 mg PS/L). In brine shrimps, NPs were observed over the body surface and within the digestive tract, associated with feces. In zebrafish, NPs were localized in the eyes, yolk sac, and tail at 72 h, showing their capacity to translocate and spread into the embryo. MP ingestion was only demonstrated for brine shrimps. In zebrafish embryos exposed to plastics-B(a)P, B(a)P appeared in the yolk sac of the embryos. The presence of B(a)P was also noticeable in brine shrimps exposed to 500 nm NPs-B(a)P. In conclusion, NPs entered and spread into the zebrafish embryo and PS NPs, and MPs were successful vectors of B(a)P to brine shrimp and zebrafish embryos. Particle size played a significant role in explaining the toxicity of plastics–B(a)P. Our study provides support for the idea that plastics may pose a risk to aquatic organisms when combined with persistent organic pollutants such as B(a)P.

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“…Abbreviations are median diameter size (D50), low-density polyethylene (LDPE), polyethylene (PE), polypropylene (PP) and polystyrene (PS). b The authors have also published an assessment of extractable organics from microplast samples ( 36 , 37 ). c Exposure to microplastics produced genotoxicity, measured by the Fpg-modified comet assay.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of Particles From Grinded Plastic Items in Caco-2 and HepG2 Cells

Roursgaard

Rothmann

Schulte

et al. 2022

Front. Public Health

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Large plastic litters degrade in the environment to micro- and nanoplastics, which may then enter the food chain and lead to human exposure by ingestion. The present study explored ways to obtain nanoplastic particles from real-life food containers. The first set of experiments gave rise to polypropylene nanoplastic suspensions with a hydrodynamic particle size range between 100 and 600 nm, whereas the same grinding process of polyethylene terephthalate (PET) produced suspensions of particles with a primary size between 100 and 300 nm. The exposure did not cause cytotoxicity measured by the lactate dehydrogenase (LDH) and water soluble tetrazolium 1 (WST-1) assays in Caco-2 and HepG2 cells. Nanoplastics of transparent PET food containers produced a modest concentration-dependent increase in DNA strand breaks, measured by the alkaline comet assay [net induction of 0.28 lesions/106 bp at the highest concentration (95% CI: 0.04; 0.51 lesions/106 base pair)]. The exposure to nanoplastics from transparent polypropylene food containers was also positively associated with DNA strand breaks [i.e., net induction of 0.10 lesions/106 base pair (95% CI: −0.04; 0.23 lesions/106 base pair)] at the highest concentration. Nanoplastics from grinding of black colored PET food containers demonstrated no effect on HepG2 and Caco-2 cells in terms of cytotoxicity, reactive oxygen species production or changes in cell cycle distribution. The net induction of DNA strand breaks was 0.43 lesions/106 bp (95% CI: 0.09; 0.78 lesions/106 bp) at the highest concentration of nanoplastics from black PET food containers. Collectively, the results indicate that exposure to nanoplastics from real-life consumer products can cause genotoxicity in cell cultures.

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Toxicity assessment of pollutants sorbed on environmental microplastics collected on beaches: Part II-adverse effects on Japanese medaka early life stages

Cited by 69 publications

References 67 publications

Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line

Toxicity assessment of pollutants sorbed on environmental sample microplastics collected on beaches: Part I-adverse effects on fish cell line

Screening of the Toxicity of Polystyrene Nano- and Microplastics Alone and in Combination with Benzo(a)pyrene in Brine Shrimp Larvae and Zebrafish Embryos

Genotoxicity of Particles From Grinded Plastic Items in Caco-2 and HepG2 Cells

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