Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies

Areecheewakul, Sudartip; Adamcakova‐Dodd, Andrea; Givens, Brittany E.; Steines, Benjamin R.; Wang, Yifang; Meyerholz, David K.; Parizek, Nathanial J.; Altmaier, Ralph; Haque, Ezazul; O’Shaughnessy, Patrick T.; Salem, Aliasger K.; Thorne, Peter S.

doi:10.1016/j.impact.2020.100214

Cited by 24 publications

(18 citation statements)

References 67 publications

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“…The results indicated that the 5 nm CuO NPs consistently decreased in hydrodynamic diameter with increasing FBS concentration, whereas the 35 nm CuO NPs only decreased in hydrodynamic diameter between 0% and 1% FBS. These results are consistent with the existing literature that serum decreases NP aggregation, [ 59–62 ] indicating that regardless of the serum concentration, the CuO NP aggregate size exposed to the cells is ≈300 nm.…”

Section: Discussionsupporting

confidence: 92%

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Morris

Givens

Silva

et al. 2021

Advanced NanoBiomed Research

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Copper oxide (CuO) nanoparticles (NPs) are abundant in manufacturing processes, but they are an airway irritant. In vitro pulmonary toxicity of CuO NPs has been modeled using cell lines such as human bronchial epithelial cell line BEAS‐2B. In 2D in vitro culture, BEAS‐2B undergoes squamous differentiation due to the presence of serum. Differentiation is part of the repair process of lung cells in vivo that helps to preserve the epithelial lining of the respiratory tract. Herein, the effects of serum on the hydrodynamic diameter, cellular viability, cellular differentiation, and cellular uptake of 5 and 35 nm CuO NPs are investigated, and the mean cell area is used as the differentiation marker for BEAS‐2B cells. The results demonstrate that the hydrodynamic diameter decreases with the addition of serum to the culture medium. Serum also increases the mean cell area, and only affects dose‐dependent cytotoxicity of 35 nm CuO NPs, while simultaneously having no effect on intracellular Cu2+. This study presents evidence that both NP size and the presence of serum in culture media influence the relative viability of BEAS‐2B cells following CuO NP exposure and highlights a critical need for carefully designed experiments and accurately reported conditions.

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Section: Discussionsupporting

confidence: 92%

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Morris

Givens

Silva

et al. 2021

Advanced NanoBiomed Research

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“…The environmental fate and toxicity of nanomaterials are critical issues in materials selection and design for soil remediation purposes. The knowledge about the environmental fate and toxicity of nanomaterials is still inadequate [48,49]. Findings of this research revealed that applying proper doses of nZVI may be beneficial for plant growth with no adverse impact on plant growth parameters; while higher doses of nZVI adversely affected the plant growth.…”

Section: Accumulation Capacity Of Pbmentioning

confidence: 88%

Effect of zero-valent iron nanoparticles on the phytoextraction ability of Kochia scoparia and its response in Pb contaminated soil

Zand

Tabrizi

2020

Environmental Engineering Research

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Nanotechnology-supported phytoremediation is a new approach in remediation of toxic metal polluted soils, but very little is known about the effects of nanoparticles on plant survival and performance in Pb-contaminated soil. Seedlings of K. scoparia were exposed to different regimes of nanoparticles of zero-valent iron (nZVI) to investigate nZVI effects on plant growth, Pb uptake and accumulation and physiological response. Results indicated that the total Pb contents in K. scoparia treated with low to moderate concentrations of nZVI (100-500 mg/kg) were higher than those in control, with the highest Pb accumulation capacity of 857.18 μg per pot obtained in soil treated with 500 mg/kg nZVI. Translocation of Pb from the roots to the shoots of K. scoparia slightly increased with nZVI content of soil from 100 to 500 mg/kg, while Pb transfer in K. scoparia was suppressed at higher nZVI doses. This might be related to the biomass reduction and decrease of chlorophyll content induced by high nZVI levels. Results provide a promising method to remediate Pb-polluted soil by applying proper amounts of nZVI to enhance phytoremediation performance. Selective interaction of plants and nZVI has great application prospects in the context of soil remediation.

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“…With increasing applications for the use of CuO NP and the resulting concern their potentially harmful effects, nanomaterial toxicity research both in vitro and in vivo has dramatically increased [ 11 , 12 ]. Previous work by our group focused on assessing the toxicity of a range of nanomaterials including ZnO, V 2 O 5 , WO 3 , Al 2 O 3 , Fe 2 O 3 , TiO 2 , MgO, and CeO 2 , and revealed CuO NPs to exert the greatest cytotoxicity in a dose-dependent manner [ 13 ]. In vitro studies have been performed to determine the toxicity mechanism at a molecular level.…”

Section: Introductionmentioning

confidence: 99%

Time course of pulmonary inflammation and trace element biodistribution during and after sub-acute inhalation exposure to copper oxide nanoparticles in a murine model

et al. 2022

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Background It has been shown that copper oxide nanoparticles (CuO NPs) induce pulmonary toxicity after acute or sub-acute inhalation exposures. However, little is known about the biodistribution and elimination kinetics of inhaled CuO NPs from the respiratory tract. The purposes of this study were to observe the kinetics of pulmonary inflammation during and after CuO NP sub-acute inhalation exposure and to investigate copper (Cu) biodistribution and clearance rate from the exposure site and homeostasis of selected trace elements in secondary organs of BALB/c mice. Results Sub-acute inhalation exposure to CuO NPs led to pulmonary inflammation represented by increases in lactate dehydrogenase, total cell counts, neutrophils, macrophages, inflammatory cytokines, iron levels in bronchoalveolar lavage (BAL) fluid, and lung weight changes. Dosimetry analysis in lung tissues and BAL fluid showed Cu concentration increased steadily during exposure and gradually declined after exposure. Cu elimination from the lung showed first-order kinetics with a half-life of 6.5 days. Total Cu levels were significantly increased in whole blood and heart indicating that inhaled Cu could be translocated into the bloodstream and heart tissue, and potentially have adverse effects on the kidneys and spleen as there were significant changes in the weights of these organs; increase in the kidneys and decrease in the spleen. Furthermore, concentrations of selenium in kidneys and iron in spleen were decreased, pointing to disruption of trace element homeostasis. Conclusions Sub-acute inhalation exposure of CuO NPs induced pulmonary inflammation, which was correlated to Cu concentrations in the lungs and started to resolve once exposure ended. Dosimetry analysis showed that Cu in the lungs was translocated into the bloodstream and heart tissue. Secondary organs affected by CuO NPs exposure were kidneys and spleen as they showed the disruption of trace element homeostasis and organ weight changes.

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Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies

Cited by 24 publications

References 67 publications

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells

Effect of zero-valent iron nanoparticles on the phytoextraction ability of Kochia scoparia and its response in Pb contaminated soil

Time course of pulmonary inflammation and trace element biodistribution during and after sub-acute inhalation exposure to copper oxide nanoparticles in a murine model

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