Toxicity Assessment of Carbon Nanomaterials in Zebrafish during Development

d’Amora, Marta; Camisasca, Adalberto; Lettieri, Stefania; Giordani, Silvia

doi:10.3390/nano7120414

Cited by 65 publications

(37 citation statements)

References 51 publications

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“…The occurrence of GO functionalization in titanium discs was evaluated by Raman spectroscopy. As depicted in Figure 1, all GO-coated samples displayed the characteristic D and G Raman bands of GO [33], confirming the presence of a GO layer on the Ti discs, in contrast with the unfunctionalized discs (Ctrl and Test) showing no Raman peaks in the 800-3200 cm −1 range. Nanomaterials 2020, 10, x FOR PEER REVIEW 6 of 17 at 450 nm and 405 nm, by means of a microplate reader (Multiskan GO, Thermo Scientific, MA, USA).…”

Section: Resultssupporting

confidence: 53%

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

et al. 2020

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Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue.

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Section: Resultssupporting

confidence: 53%

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

et al. 2020

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“…Zebrafish embryos 4–120 hpf were treated with 5, 10, 50,100 μg/mL GO (Graphenea Inc, New York, USA) in E3 (2 embryos in 1 mL medium /well) and evaluated at 4, 24, 48, 72, 96, and 120 hpf (d’Amora et al 2017) for toxicological endpoints. The test solution was refreshed every 12 h. The toxicity of GO with regard to hatching and developmental delay showed a concentration-dependent effect with 50 μg/mL and above than the controls and lower concentrations (5–10 μg/mL).…”

Section: Toxic Effects Of Gpnmentioning

confidence: 99%

“…At higher concentrations (50–100 μg/mL), the survival rate and the hatching of the embryos decreased significantly between 72 and 120 hpf. The heart beats of the larvae at 72 hpf and the frequency of movements at 96 hpf were reduced significantly in the embryos treated with 50–100 μg/mL GO (d’Amora et al 2017). In a separate study, zebrafish embryos were exposed to GO flakes (40 and 80 mg/L) in freshwater for 4–96 h and evaluated for morphological and behavioral features and also the expression of heme-oxygenase (HO) and inducible Nitric Oxide synthase (iNOS) enzymes in larvae.…”

Section: Toxic Effects Of Gpnmentioning

confidence: 99%

“…Due to excellent morphological and biological features, Lin et al (2013) proposed to use zebrafish ( Danio rerio ) as an in vivo model for the study of environmental health and safety (EHS) aspects of engineered nanomaterials and nano-related products. Since then several excellent studies based on accumulation, morphology, behavior, neurological disorders, molecular mechanisms, and other potential targets of GPN have been conducted on embryos, larvae, adults, and transgenic zebrafish (Liu et al 2014; Chen et al 2015a,b; Jiang et al 2015; Mu et al 2015; Wang et al 2015; Ren et al 2016; d’Amora et al 2017; Li et al 2017; Lu et al 2017; Soares et al 2017; Souza et al 2017; Zhang et al 2017a; Zhang et al, 2017b). Studies have also been carried out with other fish species (Japanese medaka; Oryzias latipes ) (Mullick Chowdhury et al 2014; Li et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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Due to their unique physicochemical properties, graphene-based nanoparticles (GPN) constitute one of the most promising types of nanomaterials used in biomedical research. GPN have been used as polymeric conduits for nerve regeneration, carriers for targeted drug delivery, and in the treatment of cancer via photo-thermal therapy. Moreover, they have been used as tracers to study the distribution of bioactive compounds used in healthcare. Due to their extensive use, GPN released into the environment would probably pose a threat to living organisms and ultimately to human health. Their accumulation in the aquatic environment creates problems to aquatic habitats as well as to food chains. Until now the potential toxic effects of GPN are not properly understood. Despite agglomeration and long persistence in the environment, GPN are able to cross the cellular barriers successfully, entered into the cells and able to interact with all most all the cellular sites including the plasma membrane, cytoplasmic organelles, and nucleus. Their interaction with DNA creates more potential threats to both the genome and epigenome. In this brief review, we focused on fish, mainly zebrafish (Danio rerio), as a potential target animal of GPN toxicity in the aquatic ecosystem.

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“…So far, complete toxicological evaluations and fate investigations of NAs are required before in vivo efficacy studies. Zebrafish is considered a valuable in vivo model for effective and rapid evaluation and screening of the whole-body toxicity and biodistribution of nanomaterials (D'Amora et al 2016(D'Amora et al , 2017. Zebrafish is a small size animal with high fecundity rate and external fertilization (Fako and Furgeson 2009;Ko et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Biodistribution and biocompatibility of passion fruit-like nano-architectures in zebrafish

et al. 2018

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Passion fruit-like nano-architectures (NAs) are all-in-one platforms of increasing interest for the translation of metal nanoparticles into clinics. NAs are nature-inspired disassembling inorganic theranostics, which jointly combine most of the appealing behaviors of noble metal nanoparticles with their potential organism excretion. Despite their unique and promising properties, NAs in vivo interactions and potential adverse effects have not yet been investigated. In this study, we employ zebrafish (Danio Rerio) to assess the development toxicity of NAs as well as their uptake and bioaccumulation at different stages of growth. The evaluation of multiple endpoints related to the toxicity clearly indicates that NAs do not induce mortality, developmental defects, or alterations on the hatching rate and behavior of zebrafish. Moreover, the analysis of nanostructures uptake and biodistribution demonstrates that NAs are successfully internalized and present a specific localization. Overall, our results demonstrate that NAs are able to pass through the embryos chorion and accumulate in specific tissues, exhibiting an impressive biocompatibility.

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Toxicity Assessment of Carbon Nanomaterials in Zebrafish during Development

Cited by 65 publications

References 51 publications

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

Graphene-Based Nanomaterials Toxicity in Fish

Biodistribution and biocompatibility of passion fruit-like nano-architectures in zebrafish

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