Toxicity assessment and antinociceptive activity of an ethanolic extract from Croton blanchetianus (Euphorbiaceae) leaves

Freitas, Anderson Felipe Soares de; Costa, Wêndeo Kennedy; Machado, Janaína Carla Barbosa; Ferreira, Magda Rhayanny Assunção; Paiva, Patrícia Maria Guedes; Medeiros, Paloma Lys de; Soares, Luiz Alberto Lira; Oliveira, Alisson Macário de; Napoleão, Thiago Henrique

doi:10.1016/j.sajb.2020.06.015

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…A previous study has revealed a high flavonoid content in the ethanolic extract from C. blanchetianus (used as starting material to obtain the EAF). 18 In the present study, the EAF was characterized by LC/MS, and it was possible to identify ten compounds based on the retention time (min) and mass-to-charge ratio (m/z) (Figure 1). All the compounds identified are listed in Table 1 and belonged to the group of polyphenols, especially flavonoids.…”

Section: Resultsmentioning

confidence: 97%

“…Briefly, 100 g of the leaf powder were homogenized (3 cycles of 30 s with 4 min interval) in 1 L of hydroalcoholic mixture (1 : 1 ethanol: water) and spray dried in the Mini Spray drier LM MSDi 1.0 (LabMaq, Ribeirão Preto, Brazil) to obtain the dried product (extract). [18] Next, the extract was dissolved in distilled water (10 % w/v) and partitioned (1 : 1 v/v) ten times with ethyl acetate to obtain a fraction enriched in polyphenols. The ethyl acetate phases were combined, concentrated on a rotary evaporator under reduced pressure until the total removal of the organic solvent, and dried using a L101 freeze dryer (Liotop, São Carlos, Brazil), yielding an ethyl acetate fraction (EAF).…”

Section: Fraction Preparationmentioning

confidence: 99%

“…[14] Several studies have reported on the antimicrobial, [15] antioxidant [16] and healing [17] properties of this plant. Freitas et al [18] have reported that the ethanolic extract from C. blanchetianus leaves has antinociceptive activity in mice and suggested that this effect is linked to the high flavonoids content. Further, the authors reported no signs of toxicity in the animals using acute toxicity and genotoxicity assays.…”

Section: Introductionmentioning

confidence: 99%

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Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

Oliveira

Widmer

Nascimento

et al. 2022

Chemistry & Biodiversity

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The ethanolic extract from Croton blanchetianus leaves has been shown to have antinociceptive activity in mice. Here, we investigated the antinociceptive activity of an ethyl acetate fraction (EAF) from this extract in mice and the possible pathways involved in the analgesic effect. Adverse effects on behavior and motor coordination were also evaluated. The EAF was characterized by liquid chromatography coupled with mass spectrometry and evaluated (12.5, 25, and 50 mg/kg per os) in the acetic acid-induced abdominal writhing, formalin, hot plate, and tail immersion assays. Naloxone, atropine, glibenclamide, prazosin, or yohimbine was pre-administered to mice to investigate the involved pathways in the formalin test. The open-field, rotarod, and elevated plus-maze tests were used to assess behavior and locomotion. The main components of the EAF were quercetin-3-O-(2-rhamnosyl) rutinoside, hyperoside, quercetin rutinoside pentoside, and quercetin hexoside deoxyhexoside. EAF showed antinociceptive effects in all models and was effective against both neurogenic and inflammatory pain. The reversion of the effects in the formalin test by naloxone and atropine revealed that the EAF acted via the opioid and cholinergic systems. In the open-field test, the behavior of the animals treated with the EAF was like that of control, except at the highest dose, when hypnosis, eyelid ptosis, decreased walking, hygiene, and rearing behaviors were observed. No muscle relaxant effect was observed, but an anxiogenic effect was observed at all doses. This study provides new scientific evidence on the pharmacological properties of C. blanchetianus leaves and their potential for the development of phytomedicines with analgesic properties.

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Section: Resultsmentioning

confidence: 97%

Section: Fraction Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

Oliveira

Widmer

Nascimento

et al. 2022

Chemistry & Biodiversity

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“…Groups of eight male animals were separated and received saline extract (100, 200, or 400 mg/kg per os ), MvRL‐rich fraction (25, 50, or 100 mg/kg per os ), morphine (10 mg/kg i. p.), [67] indomethacin (20 mg/kg i. p.), [67] or vehicle (0.9 %, w/v, NaCl per os ; control). After 1 h, nociception was induced by intraperitoneal injection of 0.85 % (v/v) acetic acid (0.1 mL/10 g b.w.)…”

Section: Methodsmentioning

confidence: 99%

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Silva

Oliveira

Freitas

et al. 2021

Chemistry & Biodiversity

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Previous studies have characterized a saline extract from Microgramma vacciniifolia rhizome and its lectin (MvRL)‐rich fraction with low acute toxicity. In the present study, we evaluated these preparations for acute toxicity (1,000 mg/kg) and antinociceptive and anti‐inflammatory activities (100–400 mg/kg for the extract and 25–50 mg/kg for the fraction). No signs of toxicity were observed. Both the extract and fraction increased the latency period for nociception in the hot plate assay, decreased writhing induced by acetic acid, and promoted analgesic effects in phases 1 and 2 of the formalin test. The antinociceptive mechanism was attributed to interactions with opioid receptors and K+ ATPase channels. The extract and fraction decreased carrageenan‐induced paw edema in 46.15 % and 77.22 %, respectively, at the highest doses evaluated. Furthermore, the fraction was shown to act on the bradykinin pathway. The ability to decrease leukocyte migration after treatment was also verified in the peritonitis and air pouch models. In exudates collected from air pouches, decreased tumor necrosis factor (TNF)‐α and increased interleukin (IL)‐10 levels were noted. Both the extract and fraction also effectively inhibited the development of granulomatous tissue. In conclusion, the substances investigated in this study can be used for the development of novel therapeutic options for pain and inflammatory processes.

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“…As propriedades bioativas relacionadas ao consumo desses chás estão associadas à sua composição fitoquímica. A análise fitoquímica do extrato etanólico das folhas de C. blanchetianus revelou a presença de alcalóides, açúcares redutores, derivados cinâmicos, flavonóides, saponinas, taninos condensados, terpenos, esteróides, catequinas (taninos catéquicos) [8][9][10][11].…”

Section: Introductionunclassified

Potencial bioativo, antioxidante e antimicrobiano do extrato aquoso do processo de extração do óleo essencial de folhas de Croton blanchetianus Baill

et al. 2022

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O Croton blanchetianus Baill, conhecido como marmeleiro, é rico em metabólitos secundários, como alcalóides e terpenos, que podem apresentar efeitos biológicos. Desta forma, este estudo teve como objetivo avaliar o potencial bioativo, antioxidante e antimicrobiano dos extratos aquosos resultantes do processo de extração do óleo essencial das folhas frescas e secas do C. blanchetianus. O óleo essencial foi extraído por hidrodestilação e posteriormente o extrato aquoso foi coletado e liofilizado, sendo submetido às análises. A determinação da Concentração Inibitória Mínima e Concentração Bactericida Mínima contra bactérias patogênicas de interesse em alimentos (Staphylococcus aureus, Listeria monocytogenes, Salmonella Enteritidis e Escherichia coli) foi realizada pelo método de microdiluição. O efeito dos extratos aquosos sobre as células bacterianas foi avaliado por imagens de microscopia eletrônica de transmissão. A avaliação da atividade antioxidante foi determinada pelo método ABTS e DPPH. O extrato aquoso das folhas frescas apresentou maiores teores de flavonoides e atividade antioxidante por ABTS em comparação com o extrato de folhas secas. Os compostos fenólicos, taninos e a atividade antioxidante pelo método DPPH não diferiram significativamente entre ambos os tipos de folhas. Ambos os extratos aquosos apresentaram atividade antimicrobiana apenas sobre as bactérias Gram-positivas (S. aureus e L. monocytogenes). Foram observadas alterações celulares em S. aureus e L. monocytogenes sob ação do extrato aquoso de folhas frescas e secas. Os resultados indicam a possibilidade de utilização do extrato aquoso das folhas secas de C. blanchetianus como aditivo bioativo e para melhoria da segurança de alimentos, no que diz respeito às bactérias Gram-positivas.

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Toxicity assessment and antinociceptive activity of an ethanolic extract from Croton blanchetianus (Euphorbiaceae) leaves

Cited by 15 publications

References 31 publications

Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Potencial bioativo, antioxidante e antimicrobiano do extrato aquoso do processo de extração do óleo essencial de folhas de Croton blanchetianus Baill

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