Toxicity and non-harmful effects of the soya isoflavones, genistein and daidzein, in embryos of the zebrafish, Danio rerio

Sarasquete, Carmen; Úbeda-Manzanaro, María; Ortiz-Delgado, Juan B.

doi:10.1016/j.cbpc.2018.05.012

Cited by 21 publications

(22 citation statements)

References 102 publications

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“…A similar response in terms of apoptosis and cell death as a consequence of autophagy was also reported in MCF-7 breast cancer cells when exposed to GE 100 μM [ 41 ]. Likewise, high concentrations of GE also promoted developmental toxicity and increased mortality in zebrafish ( Danio rerio ) embryos [ 42 , 43 , 44 ]. Nevertheless, as far as we know, studies in fish cells have not been reported to date.…”

Section: Discussionmentioning

confidence: 99%

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

Balbuena-Pecino

Lutfi

Riera‐Heredia

et al. 2020

IJMS

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Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

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Section: Discussionmentioning

confidence: 99%

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

Balbuena-Pecino

Lutfi

Riera‐Heredia

et al. 2020

IJMS

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“…is isoflavone inhibits the invasion and metastasis of the PTC-derived BHP10-3 cell (with RET/PTC 1 rearrangement), BCPAP, and IHH4 (with BRAF V600E mutation) by inhibiting β-catenin and EMT [46]. However, genistein upregulates most thyroid transcript signals, except for thyroid peroxidase, in zebrafish embryos, thereby indicating potential disruptions [105].…”

Section: Flavonoidsmentioning

confidence: 99%

Herbal Active Ingredients: An Emerging Potential for the Prevention and Treatment of Papillary Thyroid Carcinoma

Yang

Chen

et al. 2020

BioMed Research International

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Papillary thyroid carcinoma (PTC) is the most common subtype of differentiated thyroid cancers in Asian coastal cities, where the patients have increased risk of potentially high or excessive iodine intake. Given the high metastasis and recurrence of patients with BRAFV600E mutation, the mortality rate of thyroid cancer has recently shown an upward trend. A variety of therapies, including surgery, radiotherapy, and chemotherapy, have been used to treat thyroid cancer, but these therapies still have limitations, including postoperative complications, drug resistance, poor efficacy, or serious side effects. Recent studies have shown the potential of active ingredients derived from herbal medicine in inhibiting PTC via various cell signaling pathways. Some plant-derived compounds, such as apigenin, genistein, and curcumin, are also known to prevent and treat PTC. This article summarizes the recent advances in the structure-functional impact of anti-PTC active ingredients and their effects on PTC cells and tumor microenvironments with an emphasis on their challenges from basic research to clinical practice.

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“…All these crucial processes are modulated through oestrogen: apoptotic, proliferative and epigenetic signaling pathways, among others. It has also been reported that all fish life stages are sensitive and respond differently to both chemical and phytochemical EDCs (Pelissero et al 1991, Kiparissis et al 2003, Segner et al 2003, Sassi-Messai et al 2009, Schiller et al 2013, Sarasquete et al 2017, 2018a,b, 2019.…”

Section: Introductionmentioning

confidence: 99%

“…In a different study with medaka Oryzias latipes and zebrafish embryos exposed to different doses of genistein, different and variable response patterns were described in the transcription levels of the ERα and ERβ receptors which also varied by species and with the phytoestrogen dose (Schiller et al 2013). Due to the highly conserved sequences and very high structural homologies (DNA-binding domain [DBD] and ligand binding domain [LBD]) between ERs in humans (ERα, β) and zebrafish (ERα, β1, β2) (Menuet et al 2002, Chandrasekar et al 2010, Schaaf 2017, Asnake et al 2019, and also because of the higher expected affinity of genistein and daidzein for ERβ, in this work we analyzed the levels of expression of ERβ transcripts (β2 + β1 as a whole) in zebrafish, as has been done in studies with other fish species (Legler et al 2000, Arukwe et al 2008, Reyhanian et al 2014, Sarasquete et al 2017, 2018a.…”

Section: Introductionmentioning

confidence: 99%

“…In zebrafish and other fish species, reduced fertilisation, decreased hatching rates and reproductive imbalances have been described as the most common endocrine disorders (and/or the most toxic effects) that can be induced by endogenous factors (e.g. sex hormones) and by isoflavonoid phytoestrogens (Bennetau-Pelissero et al 2001, Kiparissis et al 2003, Kim et al 2009, Sassi-Messai et al 2009, Schiller et al 2013, Sarasquete et al 2018a. Hatching enzymes (HEs) are composed of the astacin−protease domain, and these zinc-dependent metalloproteases possess the ability to digest the envelope of the egg or chorion.…”

Section: Introductionmentioning

confidence: 99%

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Soya isoflavones, genistein and daidzein, induce differential transcriptional modulation in the ovary and testis of zebrafish Danio rerio

2020

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Most research into the effects of phytochemical isoflavones has focussed on endocrine disruptions, and especially on oestrogenic imbalances; however, little is known about their effects on other molecular signals such as transcriptional coregulators and choriolytic enzymatic pathways, which are also important in reproductive processes. In male and female zebrafish Danio rerio, the soya isoflavones genistein and daidzein (provided at 10 mg l-1 for 15 d) modulated the basal expression levels of oestrogen receptor transcripts (ERβ) in variable and differential ways. Exposure to genistein resulted in decreased levels of ERβ in the zebrafish ovary; conversely, this isoflavone increased the basal expression levels of the hatching enzyme (HE1) in both gonads. On the other hand, daidzein increased the basal expression levels of the bromodomain testis-specific gene (BRDT) in the male gonad, but not in the ovary. Both isoflavones also differentially modulated (up-down regulations) the basal expression patterns of the 3 molecular signals studied in other regions of the body (e.g. head, digestive system, skeletal musculature). Despite all these transcriptional imbalances, neither of the phytoestrogens modified gonadal histomorphology or the baseline histochemical pattern of proteins, carbohydrates and glycoconjugates distributed in either the vitelline structures or in the developing and maturing germ cells of Danio rerio.

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Toxicity and non-harmful effects of the soya isoflavones, genistein and daidzein, in embryos of the zebrafish, Danio rerio

Cited by 21 publications

References 102 publications

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

Herbal Active Ingredients: An Emerging Potential for the Prevention and Treatment of Papillary Thyroid Carcinoma

Soya isoflavones, genistein and daidzein, induce differential transcriptional modulation in the ovary and testis of zebrafish Danio rerio

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