Toxicity and Genotoxicity of Beauty Products on Human Skin Cells In Vitro

Alnuqaydan, Abdullah M; Sanderson, Barbara J.S.

doi:10.4172/2161-0495.1000315

Cited by 10 publications

(5 citation statements)

References 32 publications

(40 reference statements)

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“…Fluorescent SWNTs have recently been employed to build sensor arrays capable of detecting protein efflux from microorganisms and synthetic scaffolds for biosensing devices . Therefore, in order to study the functionality, biocompatibility, and cell toxicity of our hybrid material 3 , standard cell proliferation assays such as MTT and crystal violet assays were carried out by using human prostate cancer (PC‐3) and healthy human dermal fibroblasts (FEK4) cell lines. PC‐3 and FEK4 cells were incubated with the hybrid material 3 (glucan@CdSe[CdSe@SWNTs]), CdSe[CdSe@SWNTs] composites, and β‐ d ‐glucan for 48 h at 37 °C under normoxia (21 % O 2 and 5 % CO 2 , Figure ) and induced acute hypoxia conditions using standard CoCl 2 .6 H 2 O based assays (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of Cadmium Selenide Nanocrystals in Biocompatible Nanotubes: DFT Calculations, X‐ray Diffraction Investigations, and Confocal Fluorescence Imaging

Calatayud

Kuganathan

et al. 2018

ChemistryOpen

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The encapsulation of CdSe nanocrystals within single‐walled carbon nanotube (SWNT) cavities of varying dimensions at elevated temperatures under strictly air‐tight conditions is described for the first time. The structures of CdSe nanocrystals under confinement inside SWNTs was established in a comprehensive study, combining both experimental and DFT theoretical investigations. The calculated binding energies show that all considered polymorphs [(3:3), (4:4), and (4:2)] may be obtained experimentally. The most thermodynamically stable structure (3:3) is directly compared to the experimentally observed CdSe structures inside carbon nanotubes. The gas‐phase DFT‐calculated energy difference between “free” 3:3 and 4:2 structures (whereby 3:3 models a novel tubular structure in which both Cd and Se form three coordination, as observed experimentally for HgTe inside SWNT, and 4:2 is a motif derived from the hexagonal CuI bulk structure in which both Cd and Se form 4 or 2 coordination) is surprisingly small, only 0.06 eV per formula unit. X‐ray powder diffraction, Raman spectroscopy, high‐resolution transmission electron microscopy, and energy‐dispersive X‐ray analyses led to the full characterization of the SWNTs filled with the CdSe nanocrystals, shedding light on the composition, structure, and electronic interactions of the new nanohybrid materials on an atomic level. A new emerging hybrid nanomaterial, simultaneously filled and beta‐d‐glucan coated, was obtained by using pristine nanotubes and bulk CdSe powder as starting materials. This displayed fluorescence in water dispersions and unexpected biocompatibility was found to be mediated by beta‐d‐glucan (a biopolymer extracted from barley) with respect to that of the individual inorganic material components. For the first time, such supramolecular nanostructures are investigated by life‐science techniques applied to functional nanomaterial characterization, opening the door for future nano‐biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Encapsulation of Cadmium Selenide Nanocrystals in Biocompatible Nanotubes: DFT Calculations, X‐ray Diffraction Investigations, and Confocal Fluorescence Imaging

Calatayud

Kuganathan

et al. 2018

ChemistryOpen

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“…Next the MTT solution was mixed and incubated up to 4 h, then DMSO was added and gently shaken for 5–15 min. Absorbance was noted at 570 nm and cell viability was determined via Equation (4) [ 37 ].

…”

Section: Methodsmentioning

confidence: 99%

In Vitro and Ex Vivo Evaluation of Mangifera indica L. Extract-Loaded Green Nanoparticles in Topical Emulsion against Oxidative Stress and Aging

Khan

Mannan

et al. 2022

Biomedicines

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Although Mangifera indica L. extract (M-Ext) of the peel and kernel possesses potent antioxidant and excellent antiaging qualities, the effects are only partially seen because of the skin’s limited ability to absorb it. M-Ext was loaded into nanolipid carriers (M-NLCs) in this work to create a green topical formulation that would boost antiaging efficacy and address penetration deficit. Compound identification was done using GCMS and atomic absorption spectroscopy for heavy metals in M-Ext. M-Ext was also evaluated against oxidative stress antioxidant enzymes. The M-NLCs were fabricated and evaluated for their physicochemical characterizations. Cytotoxicity and cell permeation analysis of M-Ext and M-NLCs were carried out in fibroblasts and HaCaT cell lines. An ex vivo permeation study of M-Ext and M-NLC-loaded emulsion was performed through rat skin and the kinetic parameters were determined. Kinetic data showed that the ex vivo permeation of M-NLC-loaded emulsion through rat skin followed the Higuchi model. The safety profile was evaluated in human volunteers after written consent. Three months’ in vivo investigations were conducted using the optimized M-NLC-loaded emulsion and vehicle (B-NLC-loaded emulsion) on human cheeks for comparison. The volunteers’ skin erythema level, melanin contents, TEWL index, moisture contents, sebum level, elasticity, pH, and pore size were examined after the first, second, and third month via noninvasive techniques. There were significant findings for physicochemical characterizations and in vitro and ex vivo studies. The findings demonstrate that the green nanolipid carriers amplified the overall antioxidant effectiveness and may represent an emerging treatment strategy for oxidative stresses and aging.

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“…In the case of cosmetic products, considering the specific route of topical application, the in vitro cytotoxicity testing on skin cell lines (in particular keratinocytes and fibroblasts) can be used for primary toxicological evaluation of new ingredients such as surfactants, colorants, preservatives, and additives. 2,[23][24][25] In this study, the human keratinocyte (HaCaT cell line) was used for evaluation of the Monascus pigments skin toxicity by using in vitro MTT assay. The results of the cytotoxicity of Monascus pigments against normal human keratinocytes were shown in Table 2 which indicated that Monascus pigments are nontoxic toward the normal skin cell in the in vitro study.…”

Section: Human Erythrocytes Cytotoxicity Assaymentioning

confidence: 99%

“…However, increasing side effects of some sunscreen components has made it indispensable to search for natural photoprotectants. 2 The most commonly utilizing sunscreen ingredients such as oxybenzone linked to sun exposure triggered allergic reactions, generating free radicals, which may be associated with cell damages. 3,4 While the nanoscale TiO 2 and ZnO are responsible for the generation of a substantial amount of reactive oxygen species, which upon UV illumination causes modifications in nucleic acid bases and eventually cell death.…”

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confidence: 99%

Prospective of Monascus Pigments as an Additive to Commercial Sunscreens

Koli

Suryawanshi

Mohite³

et al. 2019

Natural Product Communications

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Red and yellow pigments from Monascus purpureus (NMCC-PF01) were evaluated to enhance sun protection factor (SPF) of commercial sunscreens and Aloe vera extract. The extracted Monascus pigments contain rubropunctamine (red pigment) and the mixture of monascin and ankaflavin (yellow pigment) as major components. Antioxidant activity and in-vitro safety of the pigments were assessed by ferric reduction potential and DPPH radical scavenging assays, human keratinocytes (HaCaT), and erythrocytes (RBCs) cytotoxicity assay, respectively. In results, SPF of commercial sunscreens showed an increase of 36.5% with red pigment compared to the 13% increase by yellow pigment. The in-vitro studies showed 67.6% ferric reducing potential and 27% DPPH radical scavenging activity, neither cytotoxic effect against human keratinocytes nor haemolytic activity. These results confirmed the safe nature of the Monascus pigments; however, in-vivo studies merit further research. In conclusion, screened pigments from Monascus purpureus may act as potential candidates to increase SPF of commercial sunscreen naturally.

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Toxicity and Genotoxicity of Beauty Products on Human Skin Cells In Vitro

Cited by 10 publications

References 32 publications

Encapsulation of Cadmium Selenide Nanocrystals in Biocompatible Nanotubes: DFT Calculations, X‐ray Diffraction Investigations, and Confocal Fluorescence Imaging

Encapsulation of Cadmium Selenide Nanocrystals in Biocompatible Nanotubes: DFT Calculations, X‐ray Diffraction Investigations, and Confocal Fluorescence Imaging

In Vitro and Ex Vivo Evaluation of Mangifera indica L. Extract-Loaded Green Nanoparticles in Topical Emulsion against Oxidative Stress and Aging

Prospective of Monascus Pigments as an Additive to Commercial Sunscreens

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