Toxicity and ecological risk assessment for two AhR agonistic pesticides mepanipyrim and cyprodinil and their metabolites

Shen, Chao; Tang, Chen; Zhu, Kongyang; He, Chengyong; Yang, Chunyan; Zuo, Zhenghong

doi:10.1007/s11356-023-26735-6

Cited by 4 publications

(2 citation statements)

References 80 publications

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“…Previous studies have shown that cyprodinil can induce heart injury in zebra sh by activating the aryl hydrocarbon receptor (AhR) (Shen et al, 2023). In this study, we investigated the cardiac developmental toxicity of zebra sh embryos and observed that ECG could rescue the pericardial anomalies caused by cyprodinil (Fig.…”

Section: Ecg Ameliorated Cyprodinil-induced Cardiac Development Defec...mentioning

confidence: 97%

(-)-Epicatechin gallate ameliorates cyprodinil-induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

Huang,

Su,

et al. 2023

Preprint

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Cyprodinil is a widely used fungicide with broad-spectrum activity, but it has been associated with cardiac abnormalities. (-)-Epicatechin gallate (ECG), a natural polyphenolic compound, has been shown to possess protective properties in cardiac development. In this study, we investigated whether ECG could mitigate cyprodinil-induced heart defects using zebrafish embryos as a model. Zebrafish embryos were exposed to cyprodinil (500 µg/L) with or without ECG (1 µM). Our results demonstrated that ECG significantly improved the survival rate, embryo movement, and hatching delay induced by cyprodinil. Furthermore, ECG effectively ameliorated cyprodinil-induced cardiac developmental toxicity, including pericardial anomaly and impairment of cardiac function. Mechanistically, ECG attenuated the cyprodinil-induced alterations in mRNA expression related to cardiac development, such as amhc, vmhc, tbx5, and gata4, as well as calcium ion channels, such as ncx1h, atp2a2a, and cdh2. Additionally, ECG was found to inhibit the activity of the aryl hydrocarbon receptor (AhR) signaling pathways induced by cyprodinil. In conclusion, our findings provide evidence for the protective effects of ECG against cyprodinil-induced cardiac developmental toxicity, mediated through the inhibition of AhR activity. These findings contribute to a better understanding of the regulatory mechanisms and safe utilization of pesticide, such as cyprodinil.

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Section: Ecg Ameliorated Cyprodinil-induced Cardiac Development Defec...mentioning

confidence: 97%

(-)-Epicatechin gallate ameliorates cyprodinil-induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

Huang,

Su,

et al. 2023

Preprint

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show abstract

“…Due to the fact that it is easily metabolized in the environment, many of its metabolites appear with unknown structures and potential effects on living organisms (Table 1). [18]. The literature data indicate that cyprodinil causes phosphorylation of extracellular signal-regulated kinase (ERK), which phosphorylates growth factors and transcription factors.…”

Section: Introductionmentioning

confidence: 99%

Selected Fungicides as Potential EDC Estrogenic Micropollutants in the Environment

Jabłońska-Trypuć,

Wydro,

Wołejko

et al. 2023

Molecules

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An increasing level of pesticide exposition is being observed as a result of the consumption of large amounts of fruits, vegetables and grain products, which are key components of the vegetarian diet. Fungicides have been classified as endocrine-disrupting compounds, but their mechanisms of action have not yet been clarified. The effect of boscalid (B), cyprodinil (C) and iprodione (I) combined with Tamoxifen (T) and 17β-estradiol (E2) on cell viability, cell proliferation, reporter gene expression, ROS content, the cell membrane’s function, cell morphology and antioxidant enzymes gene expression in MCF-7 and T47D-KBluc cell lines were investigated. The cell lines were chosen due to their response to 17β -estradiol. The selected fungicides are commonly used in Poland to protect crops against fungi. Our results revealed that the studied fungicides caused significant increases in cell viability and proliferation, and estrogenic activity was present in all studied compounds depending on their concentrations. Oxidative stress activated uncontrolled cancer cell proliferation by inducing ROS production and by inhibiting antioxidant defense. Our findings verify that the studied fungicides could possibly exhibit endocrine-disrupting properties and exposure should be avoided.

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(−)‐Epicatechin gallate ameliorates cyprodinil‐induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

Huang,

Su,

et al. 2024

Birth Defects Research

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BackgroundCyprodinil is a widely used fungicide with broad‐spectrum activity, but it has been associated with cardiac abnormalities. (−)‐Epicatechin gallate (ECG), a natural polyphenolic compound, has been shown to possess protective properties in cardiac development.MethodsIn this study, we investigated whether ECG could mitigate cyprodinil‐induced heart defects using zebrafish embryos as a model. Zebrafish embryos were exposed to cyprodinil with or without ECG.ResultsOur results demonstrated that ECG significantly improved the survival rate, embryo movement, and hatching delay induced by cyprodinil. Furthermore, ECG effectively ameliorated cyprodinil‐induced cardiac developmental toxicity, including pericardial anomaly and impairment of cardiac function. Mechanistically, ECG attenuated the cyprodinil‐induced alterations in mRNA expression related to cardiac development, such as amhc, vmhc, tbx5, and gata4, as well as calcium ion channels, such as ncx1h, atp2a2a, and cdh2. Additionally, ECG was found to inhibit the activity of the aryl hydrocarbon receptor (AhR) signaling pathways induced by cyprodinil.ConclusionsIn conclusion, our findings provide evidence for the protective effects of ECG against cyprodinil‐induced cardiac developmental toxicity, mediated through the inhibition of AhR activity. These findings contribute to a better understanding of the regulatory mechanisms and safe utilization of pesticide, such as cyprodinil.

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Toxicity and ecological risk assessment for two AhR agonistic pesticides mepanipyrim and cyprodinil and their metabolites

Cited by 4 publications

References 80 publications

(-)-Epicatechin gallate ameliorates cyprodinil-induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

(-)-Epicatechin gallate ameliorates cyprodinil-induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

Selected Fungicides as Potential EDC Estrogenic Micropollutants in the Environment

(−)‐Epicatechin gallate ameliorates cyprodinil‐induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish

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