Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae

Duan, Junchao; Yu, Yizhi; Shi, Hongbin; Tian, Linwei; Guo, Caixia; Huang, Peili; Zhou, Xianqing; Peng, Shuangqing; Sun, Zhiwei

doi:10.1371/journal.pone.0074606

Cited by 175 publications

(88 citation statements)

References 46 publications

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“…The altered larval behaviors might result from the delayed development of the CNS or an impact on the neuromuscular system. 54 Overall, our data indicate that CuO NP aqueous exposure at high doses (12.5 mg/L or above) activates xenobioticsmetabolizing enzymes, induces an inflammatory response, and shows developmental toxicity on the zebrafish liver and CNS. Further testing of long-term exposure will help us develop a thorough understanding of the environmental concerns and safe use of CuO NPs.…”

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confidence: 63%

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Zhang¹,

He²,

Wang³

et al. 2016

IJN

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Copper oxide nanoparticles (CuO NPs) are used for a variety of purposes in a wide range of commercially available products. Some CuO NPs probably end up in the aquatic systems, thus raising concerns about aqueous exposure toxicity, and the impact of CuO NPs on liver development and neuronal differentiation remains unclear. In this study, particles were characterized using Fourier transform infrared spectra, scanning electron microscopy, and transmission electron microscopy. Zebrafish embryos were continuously exposed to CuO NPs from 4 hours postfertilization at concentrations of 50, 25, 12.5, 6.25, or 1 mg/L. The expression of gstp1 and cyp1a was examined by quantitative reverse transcription polymerase chain reaction. The expression of tumor necrosis factor alpha and superoxide dismutase 1 was examined by quantitative reverse transcription polymerase chain reaction and Western blotting. Liver development and retinal neurodifferentiation were analyzed by whole-mount in situ hybridization, hematoxylin–eosin staining, and immunohistochemistry, and a behavioral test was performed to track the movement of larvae. We show that exposure of CuO NPs at low doses has little effect on embryonic development. However, exposure to CuO NPs at concentrations of 12.5 mg/L or higher leads to abnormal phenotypes and induces an inflammatory response in a dose-dependent pattern. Moreover, exposure to CuO NPs at high doses results in an underdeveloped liver and a delay in retinal neurodifferentiation accompanied by reduced locomotor ability. Our data demonstrate that short-term exposure to CuO NPs at high doses shows hepatotoxicity and neurotoxicity in zebrafish embryos and larvae.

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confidence: 63%

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Zhang¹,

He²,

Wang³

et al. 2016

IJN

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“…The Nano-SiO 2 was fully characterized in our previous studies: 14,15 the average diameter of the Nano-SiO 2 was approximately 62.1±7.2 nm. The hydrodynamic sizes and zeta potentials of Nano-SiO 2 detected in the different media (distilled water and DMEM) displayed good monodispersity.…”

Section: Characterization Of Nano-siomentioning

confidence: 98%

Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway

Duan

et al. 2014

IJN

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154

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Although nanoparticles have a great potential for biomedical applications, there is still a lack of a correlative safety evaluation on the cardiovascular system. This study is aimed to clarify the biological behavior and influence of silica nanoparticles (Nano-SiO 2 ) on endothelial cell function. The results showed that the Nano-SiO 2 were internalized into endothelial cells in a dose-dependent manner. Monodansylcadaverine staining, autophagic ultrastructural observation, and LC3-I/LC3-II conversion were employed to verify autophagy activation induced by Nano-SiO 2 , and the whole autophagic process was also observed in endothelial cells. In addition, the level of nitric oxide (NO), the activities of NO synthase (NOS) and endothelial (e)NOS were significantly decreased in a dose-dependent way, while the activity of inducible (i)NOS was markedly increased. The expression of C-reactive protein, as well as the production of proinflammatory cytokines (tumor necrosis factor α, interleukin [IL]-1β, and IL-6) were significantly elevated. Moreover, Nano-SiO 2 had an inhibitory effect on the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway. Our findings demonstrated that Nano-SiO 2 could disturb the NO/NOS system, induce inflammatory response, activate autophagy, and eventually lead to endothelial dysfunction via the PI3K/Akt/mTOR pathway. This indicates that exposure to Nano-SiO 2 is a potential risk factor for cardiovascular diseases.

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“…54 Duan and co-workers 54 did not report if or how the applied amorphous silica interacted with the chorion. Thus, while further investigations are necessary to corroborate our findings, the nanoparticlecontaining ACP dispersions we tested do not appear to show any toxic effects of significance in either fairy shrimp or zebrafish embryos.…”

Section: View Article Onlinementioning

confidence: 99%

“…metals and metal oxides 51,52 or metalloid oxides, such as amorphous silica nanomaterials. 53,54 Only a few publications consider the ecotoxicology of organic (polymer) nanomaterials, e.g. the studies from Naha and co-workers 11 and Besseling and co-workers.…”

Section: View Article Onlinementioning

confidence: 99%

Ecotoxicological assessment of nanoparticle-containing acrylic copolymer dispersions in fairy shrimp and zebrafish embryos

Galloway

Dogra

Garrett

et al. 2017

Environ. Sci.: Nano

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Nanoparticle-containing polymer dispersions are widely used, but little is known of their environmental effects. We studied the bioavailability, uptake, tissue localisation and effects of nanoparticle-containing acrylic copolymer (ACP) dispersions (mean nanoparticle sizes: 80 nm and 110 nm) in aquatic invertebrates (Thamnocephalus platyurus; fairy shrimp) and Danio rerio zebrafish embryos after aquatic exposures. Dietary exposure tests were enabled using Casper zebrafish that lack skin pigmentation allowing for bioimaging of uptake and internal distribution. Aqueous exposures of 1000 and 2500 mg L ). Similarly, dietary exposures resulted in no ecotoxicological effects. In Casper zebrafish fed with 80 nm-ACP-spiked food, hyperspectral signals derived using coherent Raman scattering (CRS) indicated that test material was present in the intestine, and possibly in the liver, but not in other organs. CRS imaging indicated that the chemical composition of the yolk sac of an 80 nm-ACP exposed zebrafish (aquatic exposure) was altered, attributed to a change in lipid metabolism, although we could not confirm with certainty that the test material was physically present in the yolk sac. These results illustrate how CRS microscopy can be used to investigate the bioaccumulation of organic nanomaterials, provided that they induce hyperspectral profiles distinct from the biological samples. In conclusion, both 80 nmand 110 nm-ACP dispersions are internalised through dietary exposure, but are not associated with significant toxic effects.

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Toxic Effects of Silica Nanoparticles on Zebrafish Embryos and Larvae

Cited by 175 publications

References 46 publications

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae

Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway

Ecotoxicological assessment of nanoparticle-containing acrylic copolymer dispersions in fairy shrimp and zebrafish embryos

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