Toxic Effects of Recombinant Tissue Plasminogen Activator on Cultured Human Corneal Endothelial Cells

Abstract

These data suggest a dose-dependent toxic effect of rtPA on HCECs in vitro. Although intracameral rtPA concentrations up to 100 mug/mL seem to be clinically safe, the use of concentrations higher than 125 microg/mL might induce irreversible cell death and should be restricted to selected cases.

Cytotoxicity of Voriconazole on Cultured Human Corneal Endothelial Cells

“…(i) Through several passages, the phenotypic properties of the HCECs and their sensitivity to toxic agents can change. (ii) In the anterior chamber, multiple mechanisms exist that protect the corneal endothelium (e.g., epithelial, fibroblast, and platelet-derived growth factors) (36). (iii) Due to metabolism in the anterior chamber, the intracameral voriconazole concentration conceivably decreases gradually; thus, exposure to sustained high concentrations of the drug for 24 h may not be common in in vivo conditions.…”

Comparison of in vitro safety profiles of vancomycin and cefuroxime on human corneal endothelial cells for intracameral use

“…14,26,27 The corneal endothelium plays a crucial role in maintaining corneal deturgescence. Regarded as nonproliferative in vivo, the number of HCECs decreases with age; the cells cannot be compensated for because of their limited regenerative capacity.…”

Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: Capsule staining properties and cytotoxicity to human cultured corneal endothelial cells

“…16,17 The present study used human corneal endothelial cells to analyze the biocompatibility of these dyes in a well-established experimental setting with regard to an intracameral use. 18,20 In addition, lens capsule staining was tested to evaluate the ability of these dyes to serve as an aid to cataract surgery. We compared methyl blue and aniline blue with 2 common dyes for capsule staining, namely patent blue and trypan blue.…”