Toxic effects of Pb on Spirodela polyrhiza (L.): Subcellular distribution, chemical forms, morphological and physiological disorders

Sha, Sha; Cheng, Menghua; Hu, Kaijie; Zhang, Wei; Yang, Yaru; Xu, Qinsong

doi:10.1016/j.ecoenv.2019.05.085

Cited by 34 publications

(9 citation statements)

References 42 publications

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“…Deficiency of membrane integrity under stress is generally linked with excessive ROS, including H 2 O 2 and O 2− and the increasing of the MDA level [43]. MDA content is supposed to an indicator of membrane integrity and injury for estimating cell damage under a variety of disadvantage conditions [45]. In this study, we show that O 2− , H 2 O 2 , and MDA content all increased, especially H 2 O 2 and MDA content.…”

Section: Ros Homeostasis In Leaf Abscission In Respond To Tdzsupporting

confidence: 50%

“…The involvement of oxidative processes leads to leaf abscission under chilling and light stresses [17]. The increase of ROS accumulation and MDA content alter superoxide dismutase (SOD) activity and catalase (CAT) activity, and damage photosynthesis and cell structure under stress conditions [45,46]. Moreover, a previous study suggested that the activated enzymatic antioxidant system contributed to ROS scavenging and inhibition of lipid peroxidation [47,48].…”

Section: Ros Homeostasis In Leaf Abscission In Respond To Tdzmentioning

confidence: 99%

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Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in Gossypium hirsutum

Jin

Wang

et al. 2020

IJMS

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Chemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducted on two cotton cultivars (CRI 12 and CRI 49) by using 100 mg L −1 TDZ at the eight-true-leaf stage. Results showed that TDZ significantly promoted the formation of leaf abscission zone and leaf abscission. Although the antioxidant enzyme activities were improved, the reactive oxygen species and malondialdehyde (MDA) contents of TDZ increased significantly compared with CK (water). The photosynthesis system was destroyed as net photosynthesis (Pn), transpiration rate (Tr), and stomatal conductance (Gs) decreased dramatically by TDZ. Furthermore, comparative RNA-seq analysis of the leaves showed that all of the photosynthetic related genes were downregulated and the oxidation-reduction process participated in leaf shedding caused by TDZ. Consequently, a hypothesis involving possible cross-talk between ROS metabolism and photosynthesis jointly regulating cotton leaf abscission is proposed. Our findings not only provide important insights into leaf shedding-associated changes induced by TDZ in cotton, but also highlight the possibility that the ROS and photosynthesis may play a critical role in the organ shedding process in other crops.Previous studies have found that ethylene accelerates organ abscission, while auxin inhibits this process [5][6][7][8][9]. Meanwhile, researchers have paid attention to the fact that multiple cell wall-degrading enzymes are activated to dissolve the cell wall and middle lamella of abscission zone, including cellulases, pecticlyases, polygalactosidases [10][11][12]. Some research has found that hydrogen peroxide (H 2 O 2 ) acts downstream from ethylene and plays a role in the cell-wall degradation process in abscission signaling [13,14].Reactive oxygen species (ROS), including H 2 O 2 , superoxide, singlet oxygen, and the hydroxyl radical, are produced in response to environmental stress [13]. Environmental stresses (such as drought, cold stress, salt stress, and pathogen attack) are often accompanied by leaf abscission [15][16][17], but the relationship between stress and abscission is rarely studied. Excessive ROS can damage cellular components, including lipids, protein, and nucleic acids [18]. ROS produced at the abscission zone site play an important role in regulating leaf abscission under drought stress in cassava (Manihot esculenta Crantz) [19]. Recent studies have found that ROS do not work in the fruit abscission zone of olives (Olea europaea L.), but only alter oxidative stress in the abscission zone of leaves and then mediate abscission induced by ethephon [20]. Previous studies have shown that insufficient carbohydrate accumulation and distribution can lead to flower and fruit abscission. For example, carbohydrate stress can induce longan (Dimocarpus longan Lour.) fruit abscissi...

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Section: Ros Homeostasis In Leaf Abscission In Respond To Tdzsupporting

confidence: 50%

Section: Ros Homeostasis In Leaf Abscission In Respond To Tdzmentioning

confidence: 99%

Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in Gossypium hirsutum

Jin

Wang

et al. 2020

IJMS

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“…Studying the subcellular distribution and speciation of Se is fundamental to understanding the mechanisms underlying Se transport and metabolism in plants. In recent decades, numerous studies have investigated the subcellular distributions of various heavy metals (such as cadmium and lead) to reveal the tolerance and detoxification strategies of plants (Sha et al, 2019;Xiao et al, 2019;Yang et al, 2019). Other studies also focused on nutrient elements (Grillet et al, 2014;Srivastava et al, 2018) and rareearth elements (Shen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Selenite Uptake and Transformation in Rice Seedlings (Oryza sativa L.): Response to Phosphorus Nutrient Status

Wang

et al. 2020

Front. Plant Sci.

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Selenite and phosphate share similar uptake mechanisms, as a phosphate transporter is involved in the selenite uptake process. However, the mechanism by which selenium (Se) transformation in plants is mediated by phosphorus (P) remains unclear. In this hydroponic study, the absorption, translocation, and biotransformation of Se in selenitetreated rice (Oryza sativa L.) seedlings were investigated under varying P nutrient status. The results showed that P-deficient cultivation increased the Se concentration in roots with Se-only treatment by 2.1 times relative to that of the P-normal condition. However, co-treating roots with additional P caused the Se concentration to decline by 20 and 73% compared to Se treatment alone under P-normal and P-deficient cultivation, respectively. A similar pattern was also observed in Se uptake by rice roots. With an Se-transfer factor elevated by 4.4 times, the shoot Se concentration was increased by 44% with additional P supply compared to the concentration under Se-only treatment of P deficiency; however, no significant differences were observed regarding P-normal cultivation. P deficiency increased the Se percentage by 28% within the cell wall, but reduced it by 60% in the soluble fraction of Se-only treated roots relative to that of the P-normal condition. Contrarily, compared with the Se-only treatment under P deficiency, additional P supply enhanced Se storage in the root soluble fraction by 1.3 times. The opposite tendency was observed for rice shoots. Moreover, P deficiency reduced the proportion of SeMet by 22%, but increased MeSeCys by 1.3 times in Se-only treated roots compared to those under the P-normal condition. Interestingly, MeSeCys was not detected when additional P was added to the two cultivation conditions. Unlike in the roots, only SeMet was generally detected in the rice shoots. The results demonstrate that the P nutrient status strongly affects the Se biofortification efficiency in rice seedlings by altering the Se subcellular distribution and speciation.

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“…1 ). The fronds were acclimatized in 10 % Hoagland's [ 56 ] growth medium under laboratory condition as per OECD 221(6500–10000 lux light irradiance, 14-h photoperiod, and 25/20 °C day/night temperature [ 57 ].After 1 week of acclimatization of plants in medium, healthy, similar-sized fronds (about 3 g) were treated with Cu +2 and Hg +2 treatment was induced by incorporating the various concentrations of CuSO4.5 H 2 O (Sigma Aldrich, C8027, ≥98%) (0.0, 1, 10, 20 and 40.0 μM) and HgCl 2 (HiMedia, GRM1067, 99%) (0.0,0.1, 0.2, 0.3 and 0.4 μM) in the medium ( Fig. 2 ).…”

Section: Methodsmentioning

confidence: 99%

Copper and mercury induced oxidative stresses and antioxidant responses of Spirodela polyrhiza (L.) Schleid

Singh

Kumar

Soni

2020

Biochemistry and Biophysics Reports

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Duckweed is recognized as a phytoremediation aquatic plant due to the production of large biomass and a high level of tolerance in stressed conditions. A laboratory experiment was conducted to investigate antioxidant response and mechanism of copper and mercury tolerance of S. polyrhiza (L.) Schleid. To understand the changes in chlorophyll content, MDA, proline, and activities of ROS-scavenging enzymes (SOD, CAT, GPOD) during the accumulation of Cu +2 and Hg +2 , S. polyrhiza were exposed to various concentrations of Cu +2 (0.0–40 μM) and Hg +2 (0.0–0.4 μM). antioxidant activity initially indicated enhancing trend with application of 10 μM Cu +2 ; 0.2 μM Hg +2 (SOD), of 20 μM Cu +2 ; 0.2 μM Hg +2 (CAT) and of 10 μM Cu +2 ;0.2 μM Hg +2 (GPOD) and then decreased consistently up to 40 μM Cu +2 and 0.4 μM Hg +2 . In the experiment chlorophyll and frond multiplication initially showed increasing tendency and decreased gradually with the application of increased metal concentration. Application of heavy metal has constantly enhanced proline and MDA content while the maximum increase was observed with the application of 40 μM Cu; 0.4 μM Hg for proline and MDA respectively. The upregulation of antioxidant enzymes and proline reveals that S. polyrhiza has strong biochemical strategies to deal with the heavy metal toxicity induced by the accumulation of Cu +2 and Hg +2 .

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Toxic effects of Pb on Spirodela polyrhiza (L.): Subcellular distribution, chemical forms, morphological and physiological disorders

Cited by 34 publications

References 42 publications

Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in Gossypium hirsutum

Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in Gossypium hirsutum

Selenite Uptake and Transformation in Rice Seedlings (Oryza sativa L.): Response to Phosphorus Nutrient Status

Copper and mercury induced oxidative stresses and antioxidant responses of Spirodela polyrhiza (L.) Schleid

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