Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro

Zhang, Hangjun; Cai, Chenchen; Wu, Yan; Ye, Binhui; Han, Li; Shou, Xiaolu; Wang, Mengdi; Wang, Jia; Jia, Xiao‐Bin

doi:10.1016/j.aquatox.2012.09.017

Cited by 23 publications

(21 citation statements)

References 41 publications

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“…Decreased sperm motility and number of sperm cells and increased the sperm abnormality rate were also observed in frog testes in vitro following MC-LR exposure [35]. Moreover, MC-LR increased ROS production and MDA content.…”

Section: Reproductive Toxicity Of Mcs On Amphibiansmentioning

confidence: 80%

See 1 more Smart Citation

A review of reproductive toxicity of microcystins

Chen

Zhang

et al. 2016

Journal of Hazardous Materials

288

126

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Section: Reproductive Toxicity Of Mcs On Amphibiansmentioning

confidence: 80%

“…But there is also substantial evidence that MCdependent damage is accompanied by oxidative stress in the liver [24][25][26][27][28][29], kidney [25,29,30], testis [29,[31][32][33][34][35], brain [36], and intestine [37].…”

Section: Introductionmentioning

confidence: 99%

A review of reproductive toxicity of microcystins

Chen

Zhang

et al. 2016

Journal of Hazardous Materials

288

126

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“…So far, however, the expression stability of rpl8 has not been validated in Rana, although rpl8 has been used as the reference gene for analyzing gene expression in some species of the Rana genus including R. nigromaculata (Gunderson et al, 2011;Hogan et al, 2007). For example, Zhang et al (2013) used rpl8 to normalize mRNA expression of sex-related genes to investigate toxic effects of microcystin-LR on R. nigromaculata using qRT-PCR, without validation of the suitability of rpl8 as the reference gene. In the present study, we for the first time described rpl8 expression during tadpole development (from stage 26 to stage 46) and demonstrated that rpl8 had little tissuespecific difference among various tissues in R. nigromaculata.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, the suitability of rpl8 as a reference gene has never been validated in any species of the Rana genus. No information on the R. nigromaculata rpl8 gene is available in the literature, despite the fact that some researchers have used rpl8 as the reference gene in R. nigromaculata research (Zhang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones

Lou

Cao

et al. 2014

Journal of Environmental Sciences

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Like Xenopus laevis, some species of the Rana genus are also used to study endocrine disrupting chemicals (EDCs). Although ribosomal protein L8 (rpl8) is the most-used reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction in Rana, its suitability as the reference gene has never been validated in any species of the Rana genus. We characterized rpl8 cDNA in Rana nigromaculata, a promising native species in East Asia for assaying endocrine disrupting effects. We found that the rpl8 cDNA consisted of 919 bp and encoded 257 amino acids, exhibiting high identities of amino acid sequence with known rpl8 in other Rana species. Then, we examined the stability of mRNA expression during development. Compared with elongation factor 1 alpha 1, another common housekeeping gene, neither stage-specific nor tissue-specific expression of the rpl8 gene was found in all tissues examined (brain, liver, intestine, tail, testis and ovary) during R. nigromaculata development. Finally, we investigated rpl8 expression under exposure to hormones. No change in rpl8 mRNA expression was found under exposure to thyroid hormone (T4) and estrogen (estradiol), whereas expression of the corresponding biomarker genes was induced.Our results show that rpl8 is an appropriate reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction for assaying EDCs using R. nigromaculata, and might also provide support for using rpl8 as a reference gene in other Rana species due to the high conservation of rpl8 among the Rana genus.

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“…While the liver is clearly one of the most affected organs, MCs also affect brain and reproductive tissues [146][147][148][149][150][151][152][153][154][155].…”

Section: Microcystinmentioning

confidence: 99%

Cyanobacterial Toxins of the Laurentian Great Lakes, their Toxicological Effects, and Numerical Limits in Drinking Water

Miller

Beversdorf

Weirich

et al. 2017

Preprint

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Cyanobacteria are ubiquitous phototrophic bacteria that inhabit diverse environments across the planet. They dominate many eutrophic lakes impacted by excess nitrogen (N) and phosphorus (P) forming dense accumulations of biomass known as cyanobacterial harmful algal blooms or cyanoHABs. Their dominance in eutrophic lakes is attributed to a variety of unique adaptations including N and P concentrating mechanisms, N fixation, colony formation that inhibits predation, vertical movement via gas vesicles, and the production of toxic or otherwise bioactive molecules. While some of these molecules have been explored for their medicinal benefits, others are potent toxins harmful to humans, animals, and other wildlife known as cyanotoxins. In humans these cyanotoxins affect various tissues, including the liver, central and peripheral nervous system, kidneys, and reproductive organs among others. They induce acute effects at low doses in the parts-per-billion range and some are tumor promoters linked to chronic diseases such as liver and colorectal cancer. The occurrence of cyanoHABs and cyanotoxins in lakes presents challenges for maintaining safe recreational aquatic environments and the production of potable drinking water. CyanoHABs are a growing problem in the North American (Laurentian) Great Lakes basin. This review summarizes information on the occurrence of cyanoHABs in the Great Lakes, toxicological effects of cyanotoxins, and appropriate numerical limits on cyanotoxins in finished drinking water.

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Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro

Cited by 23 publications

References 41 publications

A review of reproductive toxicity of microcystins

A review of reproductive toxicity of microcystins

Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones

Cyanobacterial Toxins of the Laurentian Great Lakes, their Toxicological Effects, and Numerical Limits in Drinking Water

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