Toxic effects of HgCl2 on activities of SOD, AchE and relative expression of SOD, AChE, CYP1A1 of zebrafish

He, Zhen; Wen, Ming‐Shien; Yang, Yang; Zhang, Can; Geng, Hui; Li, Xiong; Liu, De Li

doi:10.1007/s10646-014-1350-3

Cited by 19 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results of the measurement of SOD and CTL GFP reporters evidenced that there were no significant enzymatic modulation when worms were treated only with the butiá extract, which lead us to believe that the antioxidant effect observed is due to direct interaction between extracts compounds and ROS (Figure ). Also, antioxidant enzymes induction is commonly suggested as a toxicity parameter, since their needing would indicate some sort of intracellular imbalance (Agus, Sumer, & Erkoc, ; Gemelli et al, ; Zhen et al, ). Then, lack of SOD and CTL induction may also be another indicative of butiá's extract low toxicity.…”

Section: Discussionmentioning

confidence: 99%

Butiá fruit extract (Butia eriospatha) protects against oxidative damage and increases lifespan onCaenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

Butiá (Butia eriospatha) is a fruit of a palm tree belonging to the family Arecaceae, native to South America. The aim of this study was to evaluate the antioxidant potential of butiá extract using Caenorhabditis elegans as animal model. Initially, we performed survival experiments, reproduction, resistance to oxidative stress (post or pre‐treatment with paraquat or hydrogen peroxide), longevity, superoxide dismutase, and catalase GFP reporters' expression. We observed that butiá extract did not affect the worms' survival. Similarly, egg laying also showed no significant difference between treatments. None of the extract concentrations tested was able to significantly protect or reverse paraquat‐induced oxidative stress. However, they were able to reverse the oxidative damage induced by hydrogen peroxide. In addition, butiá extract increased C. elegans lifespan under stress and not per se. Our results demonstrate that the Butiá is able to extend the lifespan of the nematode C. elegans and that this effect may be mediated by an induced resistance to oxidative stress. Practical applications The practical applications of this research are to expand and bring scientific knowledge to the population about the benefits of the consumption of this native fruit from the southern region of Brazil. Many fruits and other plant foods are consumed and spread with benefits without proper scientific proof of these benefits. This fruit is widely cultivated and its production and consumption can be expanded from these results. Still, we point out that this is the first time that the benefits of this fruit are studied.

show abstract

Section: Discussionmentioning

confidence: 99%

Butiá fruit extract (Butia eriospatha) protects against oxidative damage and increases lifespan onCaenorhabditis elegans

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In Zebra sh, low dose (0.1 LC50) of HgCl 2 increased CYP1A1, but at higher doses (0.4 and 0.8 LC50), the expression of CYP1A1 was suppressed [36]. Zuotai at the concentration of 0.2 mg/mL (below LC50) could increase CYP1A1 and CYP1B1 in Zebra sh [36]. In the mouse heart, kidney and lung, HgCl 2 (2.5 mg/kg, ip) increased CYP1A1, along with other CYP450 isoforms [37].…”

Section: Zuotai In Tibetan Medicinesmentioning

confidence: 93%

“…In comparison, the effects of HgCl 2 on CYP1A expression were more dramatic. In Zebra sh, low dose (0.1 LC50) of HgCl 2 increased CYP1A1, but at higher doses (0.4 and 0.8 LC50), the expression of CYP1A1 was suppressed [36]. Zuotai at the concentration of 0.2 mg/mL (below LC50) could increase CYP1A1 and CYP1B1 in Zebra sh [36].…”

Section: Zuotai In Tibetan Medicinesmentioning

confidence: 94%

Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

Nie¹,

Xu²,

Lu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Zuotai (mainly β-HgS)-containing 70 Wei-Zhen-Zhu-Wan (70W, Rannasangpei ) is a famous Tibetan medicine for cardiovascular and gastrointestinal diseases. We have shown that 70W protected against CCl 4 hepatotoxicity. CCl 4 is metabolized via cytochrome P450 (CYP450) to produce reactive metabolites. Whether 70W has any effect on CYP450 is unknown and such effects should be compared with mercury compounds for safety evaluation. Methods: Mice were given 70W (0.15-1.5 g/kg, po), Zuotai (30 mg/kg, po), HgCl 2 (33.6 mg/kg, po) and MeHg (3.1 mg/kg, po) for 7 days. Liver RNA and protein were isolated for qPCR and Western-blot analysis. Results: 70W and Zuotai had no effects on hepatic Aryl hydrocarbon receptor (AhR) and CYP1A2, but HgCl 2 and MeHg increased Cyp1a2 mRNA and CYP1A2 protein levels;70W and Zuotai had no effects on constitutive androstane receptor (CAR) ,CYP2B andCYP2E1 expressions, but HgCl 2 increased CAR and Cyp2b10 mRNA, HgCl 2 and MeHg increased CYP2B and CYP2E1 protein expressions; 70W and mercury compounds had no apparent effects on the expression of pregnane X receptor (PXR) and Cyp3a11 mRNA, as well as CYP3A proteins. 70W and mercury compounds had no apparent effects on the expression of peroxisome proliferator-activated receptor alpha (PPARα) and CYP4A; but HgCl 2 tended to increase Cyp4a10 mRNA and CYP4A protein expressions. 70W and Zuotai had no apparent effects on the expression of farnesoid X receptor (FXR) and Cyp7a1 , while HgCl 2 and MeHg increased CYP7A1 expression. Conclusions: Zuotai and Zuotai-containing 70W at clinical doses had minimal influence on hepatic CYPs, and the effects of 70W and Zuotai on CYP and corresponding nuclear receptors are different from HgCl 2 and MeHg.

show abstract

“…Previous studies also revealed that SOD genes were involved in response to toxic challenge. For instance, in zebrafish, researchers found that low concentrations of toxic HgCl 2 exposure could significantly induce the expression of Cu/Zn-SOD in muscle, with dose-dependent enhancement of SOD activity [15]. In muscle afferent DRG (dorsal root ganglia) neurons of rats, a lower superoxide level owing to the addition of SOD could modulate the current of voltage-gated sodium channels (Na v ), the main target site under tetrodotoxin stress [16].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of SODs in Zhikong Scallop Reveals Gene Expansion and Regulation Divergence after Toxic Dinoflagellate Exposure

et al. 2019

View full text Add to dashboard Cite

As filter-feeding animals mainly ingesting microalgae, bivalves could accumulate paralytic shellfish toxins (PSTs) produced by harmful algae through diet. To protect themselves from the toxic effects of PSTs, especially the concomitant oxidative damage, the production of superoxide dismutase (SOD), which is the only eukaryotic metalloenzyme capable of detoxifying superoxide, may assist with toxin tolerance in bivalves. To better understand this process, in the present study, we performed the first systematic analysis of SOD genes in bivalve Chlamys farreri, an important aquaculture species in China. A total of six Cu/Zn-SODs (SOD1-6) and two Mn-SODs (SOD7, SOD8) were identified in C. farreri, with gene expansion being revealed in Cu/Zn-SODs. In scallops exposed to two different PSTs-producing dinoflagellates, Alexandrium minutum and A. catenella, expression regulation of SOD genes was analyzed in the top ranked toxin-rich organs, the hepatopancreas and the kidney. In hepatopancreas, which mainly accumulates the incoming PSTs, all of the six Cu/Zn-SODs showed significant alterations after A. minutum exposure, with SOD1, 2, 3, 5, and 6 being up-regulated, and SOD4 being down-regulated, while no significant change was detected in Mn-SODs. After A. catenella exposure, up-regulation was observed in SOD2, 4, 6, and 8, and SOD7 was down-regulated. In the kidney, where PSTs transformation occurs, SOD4, 5, 6, and 8 were up-regulated, and SOD7 was down-regulated in response to A. minutum feeding. After A. catenella exposure, all the Cu/Zn-SODs except SOD1 were up-regulated, and SOD7 was down-regulated in kidney. Overall, in scallops after ingesting different toxic algae, SOD up-regulation mainly occurred in the expanded Cu/Zn-SOD group, and SOD6 was the only member being up-regulated in both toxic organs, which also showed the highest fold change among all the SODs, implying the importance of SOD6 in protecting scallops from the stress of PSTs. Our results suggest the diverse function of scallop SODs in response to the PST-producing algae challenge, and the expansion of Cu/Zn-SODs might be implicated in the adaptive evolution of scallops or bivalves with respect to antioxidant defense against the ingested toxic algae.

show abstract

Toxic effects of HgCl2 on activities of SOD, AchE and relative expression of SOD, AChE, CYP1A1 of zebrafish

Cited by 19 publications

References 14 publications

Butiá fruit extract (Butia eriospatha) protects against oxidative damage and increases lifespan onCaenorhabditis elegans

Butiá fruit extract (Butia eriospatha) protects against oxidative damage and increases lifespan onCaenorhabditis elegans

Zuotai (β-HgS)-containing 70 Wei Zhen-Zhu-Wan (Rannasangpei) differs from mercury chloride and methylmercury on hepatic cytochrome P450

Genome-Wide Identification and Characterization of SODs in Zhikong Scallop Reveals Gene Expansion and Regulation Divergence after Toxic Dinoflagellate Exposure

Contact Info

Product

Resources

About