Toxic effects of glyphosate on diploid and triploid fin cell lines from Misgurnus anguillicaudatus

Re-registration Challenges of Glyphosate in the European Union

“…In general, the most sensitive cell lines appeared to be the human hematopoietic Epstein-Barr virus transformed lymphocyte Raji cells (Townsend et al, 2017), regenerative fin cell lines of fish origin , human epithelial HaCaT keratinocyte cells (ElieCaille et al, 2010;Heu et al, 2012a,b;Qin et al, 2017) and a murine neuroectodermal stem cell-like line, NE-4C . In contrast, cell types with the lowest apparent sensitivity were human choriocarcinoma cells (JEG3) (Benachour et al, 2007;Benachour and Séralini, 2009;Gasnier et al, 2009;Romano et al, 2010;Mesnage et al, 2013a), human chorioplacental cells (JAr) (Young et al, 2015), human hepatoma cells (HepG2) (Benachour et al, 2007;Benachour and Séralini, 2009;Gasnier et al, 2009Gasnier et al, , 2010, murine osteoblast precursor cells (MC3T3-E1) (Farkas et al, 2018), human embryonic kidney cells (HEK293) (Benachour et al, 2007;Benachour and Séralini, 2009;Gasnier et al, 2009;Mesnage et al, 2013a), and human primary neonate umbilical vein endothelial cells (HUVEC) (Benachour et al, 2007;Benachour and Séralini, 2009;Gasnier et al, 2009).…”

Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays

“…In agreement with previous findings, our studies also clearly demonstrated the explicit cytotoxicity of POE-15, exerted in other studies on cellular respiration and membrane integrity between 0.00155% and 0.0097% at Roundup Classic equivalent concentration [47] , [48] . The in vitro data on cytotoxicity of glyphosate and GBHs indicate that the most sensitive cell lines appeared to be human hepatopoietic Epstein-Barr virus transformed lymphocyte Raji cells [52] , human peripheral white blood cells [69] , regenerative fin cell lines from Pond loach ( Misgurnus anguillicaudatus) [70] , human epithelial HaCaT keratinocyte cells [71] and murine neuroectodermal stem-cell-like line NE-4C [51] . In contrast, cell types with lower apparent sensitivity were JEG3 [28] , [47] , [49] , [72] , murine osteoblastic cells (MC3T3-E1) [56] , human embryonic kidney cells (HEK293) [4] , [28] , [47] , [49] .…”

Evaluación de la mutagenicidad del herbicida glifosato en Prochilodus lineatus a través del test de micronúcleos

“…La línea diploide mostró un mayor índice de MN y AMN, sin embargo, en ambas líneas, diploides y triploides se observaron también daños a nivel de la cromatina como condensación, retículo endoplasmático dañado y cuerpos apoptóticos. Los resultados indicarían que el pesticida en el rango de las concentraciones empleadas presentaría un potencial riesgo, ya que inhibe la proliferación celular y provoca lesiones citotóxicas y genotóxicas en líneas celulares de peces 32 .…”