Toxic Effects of Copper on Photosystem II of Spinach Chloroplasts

Hsu, Ban-Dar; Lee, Jee-Yau

doi:10.1104/pp.87.1.116

Cited by 75 publications

(50 citation statements)

References 12 publications

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“…However, the initial fluorescence (Fo) remained unchanged (data not shown). These data are in accordance with those reported by other authors (Shioi et al 1978b, Bohner et al 1980. Hsu and Lee 1988, Samson et al 1988, Mohanty et al 1989.…”

Section: Resultssupporting

confidence: 83%

Copper effect on the protein composition of photosystem II

et al. 2000

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We provide data from in vitro experiment on the polypeptide composition, photosynthetic electron transport and oxygen evolution activity of intact photosystem II (PSII) preparations under Cu(II) toxicity conditions. Low Cu(II) concentrations (Cu(II) per PSII reaction centre unit < 230) that caused around 50% inhibition of variable chlorophyll a fluorescence and oxygen evolution activity did not affect the polypeptide composition of PSII. However, the extrinsic proteins of 33, 24 and 17 kDa of the oxygen-evolving complex of PSII were removed when samples were treated with 300 μM CuCl 2 (Cu(II) per PSII reaction centre unit = 1400). The LHCII antenna complex and D1 protein of the reaction centre of PSII were not affected at these Cu(II) concentrations. The results indicated that the initial inhibition of the PSII electron transport and oxygen-evolving activity induced by the presence of toxic Cu(II) concentrations occurred before the damage of the oxygen-evolving complex. Indeed, more than 50% inhibition could be achieved in conditions where its protein composition and integrity was apparently preserved.

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Section: Resultssupporting

confidence: 83%

Copper effect on the protein composition of photosystem II

et al. 2000

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“…Recently it has been found that at very low, equimolar Cu 2+ /PSII reaction center proportions copper stimulates oxygen evolution [2]. However, at elevated concentrations Cu(II) inhibits the photosynthetic electron transport [3][4][5]. Photosystem II is more sensitive to Cu 2+ action than photosystem I [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…However, at elevated concentrations Cu(II) inhibits the photosynthetic electron transport [3][4][5]. Photosystem II is more sensitive to Cu 2+ action than photosystem I [3][4][5]. In the literature, various sites of copper action have been suggested.…”

Section: Introductionmentioning

confidence: 99%

Mössbauer Studies of Cu(II) Ions Interaction with the Non-Heme Iron and Cytochrome b₅₅₉in a Chlamydomonas reinhardtii PSI Minus Mutant

et al. 2006

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Mössbauer spectroscopy was applied, for the first time, to study the interaction of copper ions with the non-heme iron and the heme iron of cytochrome b559 in photosystem II thylakoids isolated from a Chlamydomonas reinhardtii photosystem I minus mutant. We showed that copper ions oxidize the heme iron and change its low spin state into a high spin state. This is probably due to deprotonation of the histidine coordinating the heme. We also found that copper preserves the non-heme iron in a low spin ferrous state, enhancing the covalence of iron bonds as compared to the untreated sample. We suggest that a disruption of hydrogen bonds stabilizing the quinone-iron complex by Cu 2+ is the mechanism responsible for a new arrangement of the binding site of the non-heme iron leading to its more "tense" structure. Such a diamagnetic state of the non-heme iron induced by copper results in a magnetic decoupling of iron from the primary quinone acceptor. These results indicate that Cu does not cause removal of the non--heme iron from its binding site. The observed Cu 2+ action on the non--heme iron and cytochrome b 559 is similar to that previously observed for α-tocopherol quinone.

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“…Cd and Zn are more mobile than Cu and Pb (Greger, 2004). Zn is translocated extensively, as it is essential to plant metalloenzymes (Delhaize et al, 1985;Van Assche and Clijsters, 1990) and photosynthesis (Hsu and Lee, 1988), while Pb and Cd are toxic to plants.…”

Section: Discussionmentioning

confidence: 99%

A comparative study on plant morphology, gas exchange parameters, and antioxidant response of Ocimum basilicum L. and Origanum vulgare L. grown on industrially polluted soil

Stancheva¹,

Geneva²,

Markovska³

et al. 2014

Turk J Biol

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The effects of Cd, Pb, and Zn uptake on plant morphology, photosynthetic parameters, antioxidant potential, and essential oil yield and quality in Ocimum basilicum L. and Origanum vulgare L. plants were evaluated. The plants were grown as a pot experiment in soil heavily polluted with Cd and Pb and on unpolluted soil. Both plants accumulated Cd, mainly in the roots, while Pb occurred in the oregano shoots only. The leaf blade thickness of both plants increased when grown in polluted soil. Basil plants responded to the action of heavy metals with increases in gas exchange , stomatal conductivity, and transpiration, but water-use efficiency declined. Gas exchange and transpiration were reduced in treated oregano plants, but stomatal conductivity and water-use efficiency increased significantly. In basil, the increased levels of low molecular weight antioxidants such as phenols and flavonoids were observed, while in oregano, ascorbate, glutathione, and phenols were enhanced. Enzymatic antioxidant defense was observed in both plants when grown in contaminated soil, mainly with glutathione peroxidase, quaiacol peroxidase, glutathione S-transferase, and glutathione reductase. Accumulated levels of Cd, Pb, and Zn in plant organs resulted in a reduction in essential oil yield in basil only.

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Toxic Effects of Copper on Photosystem II of Spinach Chloroplasts

Cited by 75 publications

References 12 publications

Copper effect on the protein composition of photosystem II

Copper effect on the protein composition of photosystem II

Mössbauer Studies of Cu(II) Ions Interaction with the Non-Heme Iron and Cytochrome b₅₅₉in a Chlamydomonas reinhardtii PSI Minus Mutant

A comparative study on plant morphology, gas exchange parameters, and antioxidant response of Ocimum basilicum L. and Origanum vulgare L. grown on industrially polluted soil

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Toxic Effects of Copper on Photosystem II of Spinach Chloroplasts

Cited by 75 publications

References 12 publications

Copper effect on the protein composition of photosystem II

Copper effect on the protein composition of photosystem II

Mössbauer Studies of Cu(II) Ions Interaction with the Non-Heme Iron and Cytochrome b559in a Chlamydomonas reinhardtii PSI Minus Mutant

A comparative study on plant morphology, gas exchange parameters, and antioxidant response of Ocimum basilicum L. and Origanum vulgare L. grown on industrially polluted soil

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Product

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Mössbauer Studies of Cu(II) Ions Interaction with the Non-Heme Iron and Cytochrome b₅₅₉in a Chlamydomonas reinhardtii PSI Minus Mutant