Toxic Effects of Capsaicin on Keratinocytes and Fibroblasts

Ko, Francis; Díaz, María Alejandra; Smith, Paul K.; Emerson, Edward C.; Kim, Y J; Krizek, Thomas J.; Robson, M. C.

doi:10.1097/00004630-199809000-00010

Cited by 24 publications

(9 citation statements)

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“…In addition, Denda and coworkers reported that immunoreactive VR1 was present on epidermal keratinocytes in human skin (Denda et al, 2001;Inoue et al, 2002). Exposing cultured keratinocytes to capsaicin induces cell death (Ko et al, 1998); whereas cell death does not occur in Fig. 4.…”

Section: Discussionmentioning

confidence: 92%

Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes

Southall

Gharibova

et al. 2003

J Pharmacol Exp Ther

273

242

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During dermal injury and the associated trauma a number of compounds are released that can mediate the inflammatory response. Determining the cellular mechanisms that initiate the inflammatory responses to acute keratinocyte damage is important for understanding the regulation of epidermal inflammation. The recently cloned vanilloid receptor-1 (VR1) is a polymodal receptor, responding to thermal, pH, or vanilloids such as capsaicin stimulation. Although VR1 has been localized only on sensory neurons and within the central nervous system, recent evidence suggests a functional VR1 is expressed in human skin and epidermal cells. Using reverse transcriptionpolymerase chain reaction and immunoblotting we report that human keratinocytes and the human keratinocyte cell line HaCaT express VR1. Consistent with neuronal VR1, activation of epidermal VR1 by capsaicin induced a calcium influx. TreatingHaCaT cells with capsaicin resulted in a dose-dependent expression of cyclooxygenase-2 (COX-2), whereas pretreatment with the VR1 receptor antagonist capsazepine abolished the capsaicin-stimulated increase in COX-2 expression. Furthermore, the capsaicin-induced expression of COX-2 was dependent on extracellular calcium. Activation of the epidermal VR1 by capsaicin also resulted in an increased release of interleukin-8 and prostaglandin E 2 , and the stimulated release was attenuated by capsazepine. The finding that VR1 is expressed by keratinocytes is of great importance because it expands the putative role of VR1 beyond that of pain perception. Our results suggest that VR1 expression in keratinocytes may have a role in the inflammation that occurs secondary to epidermal damage or insult, and thus may function as a sensor for noxious cutaneous stimulation.

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Section: Discussionmentioning

confidence: 92%

Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes

Southall

Gharibova

et al. 2003

J Pharmacol Exp Ther

273

242

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“…Multiple or prolonged exposure results in desensitization to capsaicin and to heat, chemical irritation, and pain [Monsereenusorn et al, 1982]. Topical exposure, sometimes in combination with an analgesic or local anesthetic to reducing burning, has been used to give long-lived relief from pain in a wide range of debilitating medical conditions, including posttherapeutic neuralgia following shingles, arthritis, polyneuropathy, atypical facial pain, muscle ache, and postoperative pain [Ko et al, 1988]. However, absorption via human skin applications at low concentrations is unlikely to have a general desensitizing effect.…”

Section: Introductionmentioning

confidence: 99%

Examination of the potential genotoxicity of pure capsaicin in bacterial mutation, chromosome aberration, and rodent micronucleus tests

Proudlock¹,

Thompson²,

Longstaff³

2004

Environ and Mol Mutagen

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There is widespread dietary exposure to capsaicin in the form of chili peppers, while capsaicin's analgesic qualities have led to increased use of a topical herbal remedy in various impure forms. Most recently, injection of pure capsaicin has been proposed as a means of relieving a variety of debilitating diseases, in which case tissues would receive relatively high and direct exposure. The purpose of the present study, where a series of standard assays were performed in accordance with the Organisation for Economic Cooperation and Development guidance, was to clarify earlier conflicting reports concerning potential genotoxicity of capsaicin prior to administering it to patients in an injectable form. The results confirm the absence of genotoxic activity of high-purity capsaicin in the bacterial mutation and chromosome aberration tests. In addition, no evidence of cytotoxicity or genotoxicity was seen in the rat bone marrow micronucleus test, where systemic exposure to pure capsaicin was achieved using the subcutaneous route and a rising dose toleration protocol. It is concluded that pure capsaicin is not active in the standard battery of genotoxicity assays recommended by the International Conference on Harmonisation for evaluation of new medicines; earlier reported in vitro genotoxic activity is probably associated with mutagenic impurities in commercial grades of the material.

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“…Topical Capsaicin was mixed with cultures of human keratinocytes and human fibroblasts and incubated over time. At concentration of at least 0.1%, capsaicin is shown to penetrate the inner layers of this collagen mixture and be cytotoxic to both keratinocytes and fibroblasts [5]. Combined use of capsaicin, and a heating pad can result in a thermal burn.…”

Section: Discussionmentioning

confidence: 99%

Differential Diagnosis of Post-Procedural Skin Lesions: A Case Report

Waghray-Penmetcha

Puranik

et al. 2016

PST

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Skin reactions caused by interventional pain procedures are well documented in literature, ranging from fistula formation to urticarial allergic reactions and infections. Burn lesions may also occur, however far less common; and as pain physician we must be cognizant of this possible complication and its etiologies. This is difficult in an outpatient setting where a patient cannot be regularly monitored, their adherence to prescribed therapies is unclear, and reporting is often done via phone, ancillary staff, and outside facility records. These compounding factors require clinicians to consider a broad differential and be comfortable with instituting myriad therapies or appropriately involve outside consultation for thorough patient care.

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Toxic Effects of Capsaicin on Keratinocytes and Fibroblasts

Cited by 24 publications

References 0 publications

Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes

Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes

Examination of the potential genotoxicity of pure capsaicin in bacterial mutation, chromosome aberration, and rodent micronucleus tests

Differential Diagnosis of Post-Procedural Skin Lesions: A Case Report

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