Toxic Effects of Cadmium on Fertilizing Capability of Spermatozoa, Dynamics of the First Cleavage and Pluteus Formation in the Sea Urchin Anthocidaris crassispina (Agassiz)

Vaschenko, Marina A.; Zhang, Z.P; Lam, Patrick T.I.; Wu, Rongben

doi:10.1016/s0025-326x(99)00116-2

Cited by 27 publications

(9 citation statements)

References 22 publications

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“…For example in cnidarians metals may cause mucus secretion (Wooldridge 2009), zooxanthellae loss (Harland and Nganro 1990) and accumulate in zooxanthellae, tissue and skeletons (Reichelt-Brushett and McOrist 2003). Trace metals also interfere with calcium cation exchange and membrane permeability (Vaschenko et al 1999 This is the first study to demonstrate the effects of trace metals on successful asexual reproduction in the tropical symbiotic cnidarian A. pulchella. All the tested metals caused overall reductions in total live offspring numbers, and numbers of developed juvenile numbers after 28-day exposure (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…The 28-day EC50 values for cadmium based on inhibited asexual reproduction in A. pulchella show a much higher sensitivity than reproductive endpoints in several corals (Reichelt-Brushett andHarrison 1999, 2005), urchins (Thongra-ar 1997;Vaschenko et al 1999) and a shrimp (Brix et al 2006) (Table 4). For cobalt, no marine studies could be found which report more sensitive EC50 values lower than those estimated in the present study, and the 28-day EC50 values are at least one magnitude lower than sub-lethal estimates for algae, mussels or shrimp (U.S. EPA 2013).…”

Section: Relative Sensitivity Of Test Endpointmentioning

confidence: 99%

“…), affect normal behaviour(Biuki et al 2010) and delay or inhibit normal reproduction and early-life-stage development in many marine invertebrates(Thongra-ar 1997;Reichelt- Brushett and Harrison 2005;Vaschenko et al 1999).…”

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confidence: 99%

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Effects of Cd, Co, Cu, Ni and Zn on asexual reproduction and early development of the tropical sea anemone Aiptasia pulchella

2014

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Currently few studies present sub-lethal toxicity data for tropical marine species, and there are no routine toxicity tests using marine cnidarians. The symbiotic sea anemone Aiptasia pulchella has been identified as a useful species for ecotoxicological risk assessment, and would provide a tropical marine cnidarian representative. Chronic sub-lethal toxicity tests assessing the effects of 28-day trace metal exposure on asexual reproduction in A. pulchella were investigated, and concentration-dependant reductions in the number of offspring that were produced were evident for all metal exposures. Metal concentration estimates causing 50% reductions in the numbers of asexually-reproduced juveniles after 28-day exposures (28-day effect concentrations 50%: EC50s) were 14 µg/L for copper, 63 µg/L for zinc, 107 µg/L for cobalt, 145 µg/L for cadmium, and 369 µg/L for nickel. Slightly higher 28-day EC50s of 16 µg/L for copper, 192 µg/L for zinc, 172 µg/L for cobalt, 185 µg/L for cadmium, and 404 µg/L for nickel exposures and were estimated based on reductions in the total number of live developed and undeveloped offspring. These sensitive and chronic sub-lethal toxicity estimates help fill the knowledge gap related to metal effects on cnidarians over longer exposure periods, and this newly-developed bioassay may provide a much needed tool for ecotoxicological risk assessment relevant to tropical marine environments.

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Section: Discussionmentioning

confidence: 90%

Section: Relative Sensitivity Of Test Endpointmentioning

confidence: 99%

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Effects of Cd, Co, Cu, Ni and Zn on asexual reproduction and early development of the tropical sea anemone Aiptasia pulchella

2014

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“…The observed cytological alterations of sperm tails and mitochondria/midpiece may explain the decline in motility and poor resolution in sperm produced by sea urchins exposed to Cd, causing a lower percent fertilization and cleavage rate, implying that male sea urchins were more sensitive than females to chronic Cd exposure (Au et al 2001b). Reduction of egg fertilization rate and embryogenesis Gharred et al (2016) Pluteus (48 h) 0,1-10 mg/L of CdCl 2 Alterations in embryo and larva development Manzo et al (2010) Gastrula (24 h) 1000-2500 μg/L of CdCl 2 Reversibility of metal exposure effects using the delay and degree of various abnormalities Morroni et al (2018) Adults (coelomocytes) 10 −5 -10 −3 M of CdCl 2 Increase of HSP70 levels Matranga et al (2002) Blastula stage 1 mM of CdSO 4 Alterations in embryo development and increase of HSC70 levels Geraci et al (2004) Blastula (15 h Further studies by Vaschenko et al (1999) were carried out by exposing spermatozoa and eggs to different concentrations of Cd to study the effects on the fertilizing capability of spermatozoa, dynamics of the first cleavage, and pluteus formation. The exposure of sperms to Cd concentrations of 0.32, 0.56, 1.0, 1.8, 3.2, 5.6, and 10.0 mg l −1 for 30 min caused a significant reduction of the fertilizing capability of spermatozoa at all doses.…”

Section: Effects Induced By CD Stress On Other Species Of Sea Urchinmentioning

confidence: 99%

“…Results indicated that sperms of A. crassispina are more sensitive to Cd if compared with other sea urchin species. In addition, analysis of the dynamics of the first cleavage may be used as a very sensitive and useful bioassay in the studies of embryotoxicity triggered by chemical agents (Vaschenko et al 1999).…”

Section: Effects Induced By CD Stress On Other Species Of Sea Urchinmentioning

confidence: 99%

Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators

Chiarelli

Martino

Roccheri

2019

Cell Stress and Chaperones

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In recent years, researches about the defense strategies induced by cadmium stress have greatly increased, invading several fields of scientific research. Mechanisms of cadmium-induced toxicity continue to be of interest for researchers given its ubiquitous nature and environmental distribution, where it often plays the role of pollutant for numerous organisms. The presence in the environment of this heavy metal has been constantly increasing because of its large employment in several industrial and agricultural activities. Cadmium does not have any biological role and, since it cannot be degraded by living organisms, it is irreversibly accumulated into cells, interacting with cellular components and molecular targets. Cadmium is one of the most studied heavy metal inductors of stress and a potent modulator of several processes such as apoptosis, autophagy, reactive oxygen species, protein kinase and phosphatase, mitochondrial function, metallothioneins, and heat-shock proteins. Sea urchins (adults, gametes, embryos, and larvae) offer an optimal opportunity to investigate the possible adaptive response of cells exposed to cadmium, since these cells are known to accumulate contaminants. In this review, we will examine several responses to stress induced by cadmium in different sea urchin species, with a focus on Paracentrotus lividus embryos. The sea urchin embryo represents a suitable system, as it is not subjected to legislation on animal welfare and can be easily used for toxicological studies and as a bioindicator of environmental pollution. Recently, it has been included into the guidelines for the use and interpretation of assays to monitor autophagy.

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Effects of tributyltin(IV) chloride on the gametes and fertilization of Ascidia malaca (Ascidiacea: Tunicata)

Villa

Agati

Mansueto

et al. 2003

Applied Organom Chemis

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Ascidia malaca gametes before fertilization incubated in 10 À5 or 10 À7 M solutions of tributyltin(IV) chloride, TBTCl, for 3 h appear highly damaged under transmission electron microscopy observation. Also, the fertilization process is affected by the compound: the damaged spermatozoa are present in the vitelline coat and the egg does not cleave. An increase of microbodies, structurally similar to peroxisomes, have been detected in the egg peripheral cytoplasm, probably in relation to their role in alleviating damage to some cellular components. The results have shown that the reproduction of ascidians under unfavourable environmental conditions is prevented.

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Toxic Effects of Cadmium on Fertilizing Capability of Spermatozoa, Dynamics of the First Cleavage and Pluteus Formation in the Sea Urchin Anthocidaris crassispina (Agassiz)

Cited by 27 publications

References 22 publications

Effects of Cd, Co, Cu, Ni and Zn on asexual reproduction and early development of the tropical sea anemone Aiptasia pulchella

Effects of Cd, Co, Cu, Ni and Zn on asexual reproduction and early development of the tropical sea anemone Aiptasia pulchella

Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators

Effects of tributyltin(IV) chloride on the gametes and fertilization of Ascidia malaca (Ascidiacea: Tunicata)

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