Toxic effects of ammonia on the intestine of the Asian clam (Corbicula fluminea)

Zhang, Yan; Xu, Jiamin; Yan, Zhenguang; Sun, Qianhang; Huang, Yi; Wang, Shuping; Li, Shuo; Sun, Binbin

doi:10.1016/j.envpol.2021.117617

Cited by 20 publications

(5 citation statements)

References 58 publications

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“…Cao ( ShenpingCao et al, 2021 ) reported that under 50 mg/L ammonia nitrogen stress, the intestinal villi of grass carp swelled substantially and expanded in width. Zhang et al ( Zhang et al, 2021 ) reported that ammonia nitrogen exposure not only caused intestinal inflammation in Corbicula fluminea , but also led to changes in the physical structure of intestine, including vacuolation and villi defect. Consistently, similar results were found in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Nitrogen sources derived from ammonia have been utilized to assess water quality in the aquaculture industry, and it is regarded as a long-term contaminant in the aquatic environment ( Zhang et al, 2021 ). In aquaculture water environment, ammonia nitrogen exists in the form of non-ionic ammonia (NH 3 ) and ionic ammonia (NH 4 + ) which converts to each other and maintains dynamic balance under specified pH, temperature, and salinity parameters ( Kleinhenz et al, 2018 ; Hongxing et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish (Pelteobagrus fulvidraco) and induces intestinal inflammation

Liu,

Luo,

Zuo

et al. 2023

Front. Physiol.

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Nitrogen from ammonia is one of the most common pollutants toxics to aquatic species in aquatic environment. The intestinal mucosa is one of the key mucosal defenses of aquatic species, and the accumulation of ammonia nitrogen in water environment will cause irreversible damage to intestinal function. In this study, histology, immunohistochemistry, ultrastructural pathology, enzyme activity analysis and qRT-PCR were performed to reveal the toxic effect of ammonia nitrogen stress on the intestine of Pelteobagrus fulvidraco. According to histological findings, ammonia nitrogen stress caused structural damage to the intestine and reduced the number of mucous cells. Enzyme activity analysis revealed that the activity of bactericidal substances (Lysozyme, alkaline phosphatase, and ACP) had decreased. The ultrastructure revealed sparse and shortened microvilli as well as badly degraded tight junctions. Immunohistochemistry for ZO-1 demonstrated an impaired intestinal mucosal barrier. Furthermore, qRT-PCR revealed that tight junction related genes (ZO-1, Occludin, Claudin-1) were downregulated, while the pore-forming protein Claudin-2 was upregulated. Furthermore, as ammonia nitrogen concentration grew, so did the positive signal of Zap-70 (T/NK cell) and the expression of inflammation-related genes (TNF, IL-1β, IL-8, IL-10). In light of the above findings, we conclude that ammonia nitrogen stress damages intestinal mucosal barrier of Pelteobagrus fulvidraco and induces intestinal inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish (Pelteobagrus fulvidraco) and induces intestinal inflammation

Liu,

Luo,

Zuo

et al. 2023

Front. Physiol.

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“…Typically, ammonia is one of the main metabolites originated by gut microorganisms, however, the increase of ammonia level in hepatic disorder not only aggravates brain dysfunction, but also damages intestinal barrier and alters intestinal microbial composition. [ 43,44 ] Virtually, treatment with COS influenced the gut microbiota structure of HE mice profoundly, the significantly higher abundance in CTL and CSH groups was mainly presented in a same phylum (Proteobacteria). The abundance of Muribaculaceae ( n = 3), Lactobacillus ( n = 1) and Enterorhabdus ( n = 1) were reinforced in HE group in comparison with CTL group.…”

Section: Discussionmentioning

confidence: 99%

Chitooligosaccharides Attenuated Hepatic Encephalopathy in Mice through Stabilizing Gut–Liver–Brain Disturbance

Liu

et al. 2022

Molecular Nutrition Food Res

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Scope: Hepatic encephalopathy (HE) refers to neurological dysfunction associated with hepatic inadequacy and gut dysbiosis. Chitooligosaccharides (COS) possesses prominent biological activities including incalculable hepatoprotective, neuroprotective and prebiotic effects. This study evaluates the protective effects of COS on HE from the influence of gut-liver-brain axis in mice. Methods and results: Hepatic injured mice show minimal symptoms of HE, reflecting in cognitive impairment, and learning and memory retardation, while they are reversed by COS following orally administrated. Furthermore, COS ameliorates brain function through inhibiting microglial and astrocyte activation in cerebral cortex and hippocampus, promoting neuronal regeneration characterized by the increase of neuron-specific marker (neuronal nuclear antigen, NeuN). Concurrently, neuroinflammation and hepatitis are restrained by COS through descending toll-like receptors 4/Nuclear factor kappa B (TLR4/NF-𝜿B) pathway. Additionally, the dysbiosis of the composition and structure of gut microbiota is displayed in mice with HE, while it is modified by COS through decreasing the relative abundances of Muribaculaceae, Lactobacillus, and Enterorhabdus. The enhancement of blood ammonia is crucially slipped to basal levels by COS. Conclusion: The present study shows that COS could prevent the pathological process of HE through regulating the gut-liver-brain cross-talk, which provids new insight into fundamental roles of COS.

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“…In fact, between-species comparison also displayed inconsistent ammonia impacts on intestinal microbial diversity. For example, exposure to different levels of ammonia decreased the microbial community diversity of Pacific white shrimps, Litopenaeus vannamei (20 mg/L total ammonia-N, [ 5 ]), Asian clams, Corbicula fluminea (25 mg/L, [ 35 ]), yellow catfish, Pelteobagrus fulvidraco (146 mg/L, [ 36 ]), and striped-neck turtles, Mauremys sinensis (200 mg/L, [ 20 ]), but had no significant impact on crucian carps, Carassius auratus (50 mg/L, [ 37 ]). Accordingly, the toxic effects of ammonia exposure on gut microbiota could not be simply reflected by reduced microbial diversity.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

Meng

Wang

et al. 2022

Biology

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As the most common pollutant in aquaculture systems, the toxic effects of ammonia have been extensively explored in cultured fish, molluscs, and crustaceans, but have rarely been considered in turtle species. In this study, juveniles of the invasive turtle, Trachemys scripta elegans, were exposed to different ammonia levels (0, 0.3, 3.0, and 20.0 mg/L) for 30 days to evaluate the physiological, gut microbiomic, and liver metabolomic responses to ammonia in this turtle species. Except for a relatively low growth rate of turtles exposed to the highest concentration, ammonia exposure had no significant impact on the locomotor ability and gut microbial diversity of turtles. However, the composition of the microbial community could be altered, with some pathogenic bacteria being increased in ammonia-exposed turtles, which might indicate the change in their health status. Furthermore, hepatic metabolite profiles via liquid chromatography–mass spectrometry revealed extensive metabolic perturbations, despite being primarily involved in amino acid biosynthesis and metabolism. Overall, our results show that ammonia exposure causes gut dysbacteriosis and disturbs various metabolic pathways in aquatic turtle species. Considering discrepant defense mechanisms, the toxic impacts of ammonia at environmentally relevant concentrations on physiological performance might be less pronounced in turtles compared with fish and other invertebrates.

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Toxic effects of ammonia on the intestine of the Asian clam (Corbicula fluminea)

Cited by 20 publications

References 58 publications

Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish (Pelteobagrus fulvidraco) and induces intestinal inflammation

Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish (Pelteobagrus fulvidraco) and induces intestinal inflammation

Chitooligosaccharides Attenuated Hepatic Encephalopathy in Mice through Stabilizing Gut–Liver–Brain Disturbance

Gut Microbial Composition and Liver Metabolite Changes Induced by Ammonia Stress in Juveniles of an Invasive Freshwater Turtle

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