Toxic, cytotoxic and genotoxic effect of saline extract and fraction of Parkia pendula seeds in the developmental stages of Biomphalaria glabrata (Say 1818 – intermediate host) and cercaricide activity against the infectious agent of schistosomiasis

Batista, José Josenildo; Araújo, Hallysson Douglas Andrade de; Aguiar, Thierry Wesley de Albuquerque; Ferreira, Silvio C.; Lima, Maíra de Vasconcelos; Pereira, Dewson Rocha; Ferreira, Magda Rhayanny Assunção; Soares, Luiz Alberto Lira; Melo, Ana Maria Mendonça de Albuquerque; Albuquerque, Mônica Camelo Pessôa de Azevedo; Aires, André de Lima; Coelho, Luana Cassandra Breitenbach Barroso

doi:10.1016/j.actatropica.2022.106312

Cited by 3 publications

(11 citation statements)

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“…Apoptosis is a self-induced mechanism of death and an important physiological process for homeostasis and immune response [ 54 ]. Similar results are presented with potassium usnate [ 36 ] and extract and fraction of P. pendula [ 35 ], demonstrating the importance of the study with AP and BN cells to evaluate the toxic effects on B. glabrata hemocytes. Thus, the preparations showed significant cytotoxic effects in the highest concentrations evaluated.…”

Section: Resultssupporting

confidence: 74%

“…All B. glabrata snails that died during exposure demonstrated intense retraction, mucus production and hemolymph release. Similar alterations, such as tissue damage and loss of biological functions to B. glabrata snails, were observed with exposure to the saline extract and fraction of P. pendula [ 35 ].…”

Section: Resultsmentioning

confidence: 68%

“…Furthermore, other studies also suggest that damage to DNA and other macromolecules may result from superoxide, hydrogen peroxide and hydroxyl radicals that result from cellular metabolism [ 59 ]. Thus, we can suggest that the various rates of DNA damage seen with our preparations may be corroborating oxidative stress, which in an uncontrolled way results in a breakdown of the DNA tape [ 35 , 60 ]. Studies also reported an increase in DNA damage rates in groups treated with household slat [ 61 ], sodium selenium [ 62 ] and aqueous extract of Anagallis arvensis [ 63 ], which corroborates our results.…”

Section: Resultsmentioning

confidence: 76%

“…Among the malformations observed, developmental delays stand out ( Figure 3 M), as well as hydropic embryos ( Figure 3 P) and shell malformations ( Figure 3 N). According to the study using saline extract and fraction enriched with lectin from Parkia pendula seeds [ 35 ], the different alterations presented after exposure may be associated with the capacity of protein/lectin to bind to different carbohydrates, causing damage, inhibition of hatching and death of these embryos. Embryos with teratogenic effects rarely hatch [ 36 ], thus, the presence of morphological alterations in embryos exposed to B. monandra preparations demonstrate the capacity to inhibit development and hatching, consequently causing population control of this host.…”

Section: Resultsmentioning

confidence: 99%

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Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

et al. 2022

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Biomphalaria glabrata snails constitute the main vector of schistosomiasis in Brazil, and Bauhinia monandra Kurz, the leaves of which contain BmoLL lectin with biocidal action, is a plant widely found on continents in which the disease is endemic. This work describes the composition of B. monandra preparations and the effect on embryos and adult snails, their reproduction parameters and hemocytes. We also describe the results of a comet assay after B. glabrata exposure to sublethal concentrations of the preparations. Additionally, the effects of the preparations on S. mansoni cercariae and environmental monitoring with Artemia salina are described. In the chemical evaluation, cinnamic, flavonoid and saponin derivatives were detected in the two preparations assessed, namely the saline extract and the fraction. Both preparations were toxic to embryos in the blastula, gastrula, trochophore, veliger and hippo stages (LC50 of 0.042 and 0.0478; 0.0417 and 0.0419; 0.0897 and 0.1582; 0.3734 and 0.0974; 0.397 and 0.0970 mg/mL, respectively) and to adult snails (LC50 of 6.6 and 0.87 mg/mL, respectively), which were reproductively affected with decreased egg deposition. In blood cell analysis, characteristic cells for apoptosis, micronucleus and binucleation were detected, while for comet analysis, different degrees of nuclear damage were detected. The fraction was able to cause total mortality of the cercariae and did not present environmental toxicity. Therefore, B. monandra preparations are promising in combating schistosomiasis since they can control both the intermediate host and eliminate the infectious agent, besides being safe to the environment.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

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Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

et al. 2022

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“…Artemia salina is widely used in the toxicity assessment of natural or synthetic molecules with biotechnological potential, as in molluscicide screening. This biological model is used for being a safe, practical, cost‐effective and highly sensitive method 27–31 . Therefore, this study reports for the first time the toxicity of plumbagin against embryos (blastula, gastrula, trochophore, veliger, and hippo stage) and adults of B. glabrata , its cytotoxic and genotoxic effect, cercaricidal activity against cercariae of S. mansoni , and ecotoxicological evaluation against Artemia salina .…”

Section: Introductionmentioning

confidence: 99%

Toxic, cytotoxic and genotoxic effect of plumbagin in the developmental stages of Biomphalaria glabrata (Say, 1818‐intermediate host) and cercaricidal activity against the infectious agent of schistosomiasis mansoni

França

Silva

Diniz

et al. 2022

Pest Management Science

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BACKGROUND: Snails of the genus Biomphalaria are intermediate hosts of Schistosoma mansoni, the main etiological agent of schistosomiasis mansoni, which affects about 236.6 million people in tropical and subtropical regions of the world. The World Health Organization recommends the population control of vector snails as one of the strategies to reduce the prevalence and incidence of schistosomiasis. In this study, molluscicidal and antiparasitic activities of plumbagin, a naturally sourced naphthoquinone with a range of biological effects, were evaluated against B. glabrata and cercariae of S. mansoni. RESULTS:After 24 h of exposure, plumbagin demonstrated molluscicidal activity at low concentrations against embryos (LC 50 of 0.56, 0.93, 0.68, 0.51 and 0.74 ∼g mL −1 for the blastula, gastrula, trochophore, veliger and hippo stage, respectively) and adult snails (LC 50 of 3.56 ∼g mL −1 ). There were no changes in exposed snails' fecundity or fertility; however, plumbagin was able to increase the frequency of DNA damage and the number of hemocytes, with apoptosis and binucleation being the main hemocyte alterations. In addition, plumbagin showed death of S. mansoni cercariae in the concentration of 1.5 ∼g mL −1 in 60 min, while showing moderate toxicity to Artemia salina.CONCLUSION: Plumbagin proved to be a promising substance for the control of B. glabrata population, intermediate host of S. mansoni, as well as the cercariae, infective stage for humans (definitive host), while being moderately toxic to A. salina, a crustacean widely used in ecotoxicity tests.

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Photocatalytic efficacy of pyrite in the degradation of antiretroviral drugs: Biomphalaria glabrata as a bioindicator of toxic and genotoxic effects

Silva,

de Vasconcelos Lima,

Pereira

et al. 2025

Emerging Contaminants

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Toxic, cytotoxic and genotoxic effect of saline extract and fraction of Parkia pendula seeds in the developmental stages of Biomphalaria glabrata (Say 1818 – intermediate host) and cercaricide activity against the infectious agent of schistosomiasis

Cited by 3 publications

References 64 publications

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

Effect of Bauhinia monandra Kurz Leaf Preparations on Embryonic Stages and Adult Snails of Biomphalaria glabrata (Say, 1818), Schistosoma mansoni Cercariae and Toxicity in Artemia salina

Toxic, cytotoxic and genotoxic effect of plumbagin in the developmental stages of Biomphalaria glabrata (Say, 1818‐intermediate host) and cercaricidal activity against the infectious agent of schistosomiasis mansoni

Photocatalytic efficacy of pyrite in the degradation of antiretroviral drugs: Biomphalaria glabrata as a bioindicator of toxic and genotoxic effects

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