Toxic and genotoxic effects of Roundup on tadpoles of the Indian skittering frog (Euflictis cyanophlyctis) in the presence and absence of predator stress

Yadav, Sushama Singh; Giri, Sarbani; Singha, Utsab; Boro, Freeman; Giri, Anirudha

doi:10.1016/j.aquatox.2013.01.016

Cited by 52 publications

(32 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar malformations have been reported earlier in D. melanostiscus tadpoles after exposure to some organophosphate insecticides and herbicides (Jayawardena et al 2011;Wijesinghe et al 2011). These deformed and abnormally behaved tadpoles could be highly vulnerable in the wild to increased predation due to reduced ability to evade predators (Relyea 2005;Yadav et al 2013). In the present study, the lowest tested concentration of carbosulfan which induced deformities in the D. melanostiscus tadpoles after 96 h exposure was 0.6 mg/L which is the estimated LOEC for body ChE inhibition indicating the association between the development of deformities and the ChE inhibition.…”

Section: Discussionsupporting

confidence: 58%

“…D. melanostictus often breed in temporary habitats, particularly in agricultural areas and human altered habitats (IUCN 2004) where water may dry up before completion of the metamorphosis which could lead to increased mortality. Furthermore longer development period increases the chances of exposure to predators and parasites in the environment leading to increased mortality (Relyea 2005;Yadav et al 2013).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Survival and cholinesterase activity of Asian common toad (Duttaphrynus melanostictus) tadpoles following short term exposure to a carbosulfan-based pesticide

Samarakoon

Pathiratne

2017

Sri Lanka J. Aquat.

View full text Add to dashboard Cite

Carbosulfan, a carbamate pesticide widely used in agriculture especially in the Asian region has received less attention in ecological impact assessments particularly to non-target amphibians. In this study, short term effects of a carbosulfan-based pesticide (Marshal ® ) on survival and cholinesterase (ChE) activity of tadpoles of Duttaphrynus melanostictus, a non-target amphibian in tropical Asia were evaluated. Based on the concentration-toxicity response patterns, estimated median lethal concentration (LC50) and median ChE inhibitory concentration (IC50) for 96 h were 24 and 2.1 mg/L carbosulfan respectively. For the ChE inhibition for 96 h, the lowest observable effect concentration and no observable effect concentrations of carbosulfan were 0.6 and 0.3 mg/L respectively. Upon transfer to pesticide-free water by 14 days, ChE activities of the tadpoles were restored to normal levels. Since the tadpoles exposed to 0.3 mg/L carbosulfan have not exhibited lethality, morphological deformities, abnormal behavior or statistically significant ChE depression, it is unlikely that the carbosulfan-based pesticide could induce neurotoxic effects for D. melanostictus tadpoles at the concentration of ≤ 0.3 mg/L of active ingredient under short term exposure.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Survival and cholinesterase activity of Asian common toad (Duttaphrynus melanostictus) tadpoles following short term exposure to a carbosulfan-based pesticide

Samarakoon

Pathiratne

2017

Sri Lanka J. Aquat.

View full text Add to dashboard Cite

show abstract

“…Among the pesticides applied during the sample collection were glyphosate -Roundup (herbicide), alto-100 (fungicide); dimethoate, MD (insecticide and acaricide); atrazine (herbicide), carbofuran -Furadan (insecticide), Lannate (insecticide) and malathion (pesticide). Indeed, there are several studies with tadpoles, under controlled laboratory conditions, that have drawn attention to the cytotoxic, mutagenic and genotoxic effects of these pesticides and their susceptibility may vary depending on the studied species (Howe et al 2004, Relyea 2005, Relyea and Jones 2009, Bernal et al 2009, Bosch et al 2011, Meza-Joya et al 2013Yadav et al 2013). Our study, however, differs from those in that it measured genomic damages in animals exposed in their natural habitat, in real conditions of contamination.…”

Section: Discussioncontrasting

confidence: 42%

The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay

Gonçalves¹,

Gambale²,

Godoy³

et al. 2017

Zoologia

View full text Add to dashboard Cite

ABSTRACT. Amphibians inhabiting agricultural areas are constantly exposed to large amounts of chemicals, which reach the aquatic environment during the rainy season through runoff, drainage, and leaching. We performed a comet assay on the erythrocytes of tadpoles found in the surroundings of agricultural fields (soybean and corn crops), where there is an intense release of several kinds of pesticides in different quantities. We aimed to detect differences in the genotoxic parameters between populations collected from soybeans and cornfields, and between them and tadpoles sampled from non-agricultural areas (control group). Tadpoles collected from ponds located at soybean fields had significantly more DNA damage, followed by tadpoles collected from cornfields. In contrast, animals sampled from non-agricultural areas had the lowest incidence of DNA damage. In addition, we found a negative correlation between the parameters of the comet assay and the area of the ponds surrounding soybean. This correlation indicates a possible dilution effect in the concentration of pesticides. Finally, Physalaemus cuvieri Fitzinger, 1826 seems to be a good bioindicator for detecting the genotoxic effects of field agricultural insecticides; therefore, we suggest that this species should be used in environmental biomonitoring studies, since it is common and abundant where it occurs.

show abstract

“…Differential toxicity of glyphosate and its formulated PPPs have been also considered in the official scientific opinion by EFSA on pesticide risk assessment for amphibians and reptiles (Ockleford et al, 2018). Identification of acute lethal, physiological and genotoxic effects of glyphosatebased herbicides, including Roundup Original R and Roundup Transorb R , have continued on amphibians (Lajmanovich et al, 2011(Lajmanovich et al, , 2013(Lajmanovich et al, , 2014Yadav et al, 2013;Bellantuono et al, 2014;Levis and Johnson, 2015;Gandhi and Cecala, 2016;Rissoli et al, 2016;Soloneski et al, 2016) and reptiles (Latorre et al, 2013;Siroski et al, 2016). Teratogenic effects were reported in a treefrog (Scinax nasicus) (Lajmanovich et al, 2003) and in the embryo of the African clawed frog (Xenopus laevis) (Paganelli et al, 2010) in response to exposure to Glyfos R or Roundup Classic R at levels corresponding to 3-7.5 and 96-160 mg/l glyphosate concentrations, respectively.…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

104

View full text Add to dashboard Cite

Toxic and genotoxic effects of Roundup on tadpoles of the Indian skittering frog (Euflictis cyanophlyctis) in the presence and absence of predator stress

Cited by 52 publications

References 51 publications

Survival and cholinesterase activity of Asian common toad (Duttaphrynus melanostictus) tadpoles following short term exposure to a carbosulfan-based pesticide

Survival and cholinesterase activity of Asian common toad (Duttaphrynus melanostictus) tadpoles following short term exposure to a carbosulfan-based pesticide

The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay

Re-registration Challenges of Glyphosate in the European Union

Contact Info

Product

Resources

About