Towards whole sheep ovary cryopreservation

Ewe Ovarian Tissue Vitrification: A Model for the Study of Fertility Preservation in Women

“…Several other studies were conducted on the vitrification of sheep whole ovaries (Courbiere et al, 2006;2009). Baudot et al (2007) tested the VS4 solution previously described by Kheirabadi & Fahy (2000) for whole ovary vitrification and obtained reasonable rates of follicular viability (61.4 ± 2.2%) and normal morphology (48 ± 3.8%). Also in 2007, Courbiere et al demonstrated that the reestablishment of hormone production was also maintained after the transplant of whole vitrified ovaries.…”

New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

“…In fact, DSC allows the detection of phase transitions, both crystallization or glass transition, and can thus determine three thermal properties of vitrification solutions: the vitreous transition temperature, the critical cooling rate and the critical warming rate (Baudot et al, 2007). These three thermal properties are specific to each vitrification solution and could be used for a better utilization of the solutions.…”

“…On the basis of the contribution of DSC in other fields of cryopreservation [vitrification of ovary (Baudot et al, 2007), slow-freezing or vitrification of plants (Volk and Walters, 2006;Skyba et al, 2011), slow-freezing of aquatic crustaceans (Issartel et al, 2006)], our team chose a thermodynamic approach to study slow-freezing solutions for ovarian tissue. In fact, DSC allows the measure of the maximal quantity of ice formed in a solution (Q max ).…”

Preservation of female fertility in humans and animal species

“…This fertility preservation strategy involves the cryopreservation of not only the intact, whole ovary but also its supporting blood vessels (Martinez-Madrid et al, 2004;Baudot et al, 2007). Evidence to support the concept of the slow freezing and transplantation of whole ovaries and their supporting vasculature has been provided in sheep with the total restoration of natural fertility in all of the treated animals with 64% live birth rate following whole organ preservation and transplantation (Onions et al, 2009(Onions et al, , 2013Campbell et al, 2014).…”