Towards understanding molecular modes of probiotic action

Marco, Maria L.; Pavan, Sonia; Kleerebezem, Michiel

doi:10.1016/j.copbio.2006.02.005

Cited by 286 publications

(200 citation statements)

References 50 publications

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“…Both studies suggested that the pH-lowering effects of the probiotic appeared to be important for enabling these anti-infectious activities. Furthermore, B. breve strains have been reported to display antimicrobial activity against pathogens through competitive exclusion (Lievin et al, 2000), whereby probiotic strains with similar adherence mechanisms inhibit the attachment and colonization of the pathogen at these binding sites, thus protecting the host from infection (Marco et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003

et al. 2007

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Section: Discussionmentioning

confidence: 99%

Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003

et al. 2007

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“…They are important components of the gut microbiota, and they may be present at concentrations of 10 9 210 11 cells per g faeces, representing the majority of the microbial gut population in the colon during the early stage of life (Harmsen et al, 2000;Langendijk et al, 1995). Furthermore, bifidobacteria are target species for prebiotics, and some strains are known to beneficially modulate several host-cell functions, the most prevalent of which are the immune responses and intestinal barrier integrity, although the molecular basis of these promoting effects is still largely unknown (Marco et al, 2006;Salminen et al, 2005). For all these reasons, these micro-organisms are attractive probiotic candidates for inclusion in functional food products, normally being consumed as adjunct cultures in fermented dairy products (Masco et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment

et al. 2009

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Host-bacteria interactions are often mediated via surface-associated proteins. The identification of these proteins is an important goal of bacterial proteomics. To address how bile can influence the cell-envelope proteome of Bifidobacterium longum biotype longum NCIMB 8809, we analysed its membrane protein fraction using stable isotope labelling of amino acids in cell culture (SILAC). We were able to identify 141 proteins in the membrane fraction, including a large percentage of the theoretical transporters of this species. Moreover, the envelope-associated soluble fraction was analysed using different subfractionation techniques and differential in-gel fluorescence electrophoresis (DIGE). This approach identified 128 different proteins. Some of them were well-known cell wall proteins, but others were highly conserved cytoplasmic proteins probably displaying a 'moonlighting' function. We were able to identify 11 proteins in the membrane fraction and 6 proteins in the envelope-associated soluble fraction whose concentration varied in the presence of bile. Bile promoted changes in the levels of proteins with important biological functions, such as some ribosomal proteins and enolase. Also, oligopeptide-binding proteins were accumulated on the cell surface, which was reflected in a different tripeptide transport rate in the cells grown with bile. The data reported here will provide the first cell-envelope proteome map for B. longum, and may contribute to understanding the bile tolerance of these bacteria

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“…With a targeted use of specific probiotic strains, it is possible to induce an immune stimulant type of response on both B (increase of humoral immunity) and T lymphocytes (increase of cell-mediated immunity) and on the phagocytic component, particularly on polymorphonuclear cells [37,38]. The ability to stimulate responses of this type is useful in very specific clinical circumstances, for example for immunoprophylaxis of upper respiratory tract infections during winter or, in addition to influenza vaccination, to increase antibody response to vaccine or, more generally, to increase nonspecific immunity surveillance to pathogens of different nature [39,40].…”

Section: Probiotics and The Immune Responsementioning

confidence: 99%

Probiotics and health: An evidence-based review

Aureli

Capurso

Castellazzi

et al. 2011

Pharmacological Research

232

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a b s t r a c tThe intestinal microbiota is an ecosystem formed by a variety of ecological niches, made of several bacterial species and a very large amount of strains. The microbiota is in close contact with the intestinal mucosa or epithelial interface which is, after the respiratory area, the largest surface of the body, occupying approximately 250-400 m 2 . The physiological activities of the microbiota are manifold and are just being unraveled. Based on the observations of the multiple roles played by the microbiota in health and disease, the notion of modifying it with appropriate formulations, i.e. probiotics, is being tested in several settings.This review summarizes the current knowledge on probiotics and discusses both limitations and acquired evidence to support their use in preventive and therapeutic medicine.

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Towards understanding molecular modes of probiotic action

Cited by 286 publications

References 50 publications

Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003

Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003

The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment

Probiotics and health: An evidence-based review

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