Towards the production of natural rubber-calcium phosphate hybrid for applications as bioactive coatings

Nascimento, Rodney Marcelo do; Paula, Amauri J.; Oliveira, Naiara C.; Alves, Ana Cecília; Aquino, Yasmine Maria Lima de Oliveira; Filho, A. G. Souza; Rodrigues, João Elias Figueiredo Soares; Hernandes, Antônio Carlos

doi:10.1016/j.msec.2018.09.048

Cited by 10 publications

(10 citation statements)

References 34 publications

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“…In addition, NRL film showed the presence of the following chemical elements: point (4) calcium, oxygen and gold; point (5) calcium, oxygen, phosphor, potassium and sulphur; and point (6) calcium, oxygen and gold (Figure 10b). These results confirm several deposited particles, especially calcium, phosphorus and potassium, which are well-known functional materials for tissue regeneration 90 . Gold, on other hand, is frequently used for recovery of samples prior to the analysis on the microscope.…”

Section: Elemental Analysis Through Energy Dispersive Spectroscopy (Eds)supporting

confidence: 82%

Natural Rubber Films Incorporated with Red Propolis and Silver Nanoparticles Aimed for Occlusive Dressing Application

et al. 2021

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In this work, NRL films were enriched with red propolis and silver nanoparticles with the aim of obtaining dressings with bactericide properties. To improve the properties of these materials, plasma treatment was also applied to the films. Cytotoxic effects were evaluated by MTT and agar diffusion assays on secondary and stem cells. Determination of cell behavior on the films were assessed by EDS and FEG-SEM. In general, the plasma modified NRL films presented hydrophilic character, favorable condition for biomedical applications. RBS results showed that silver is probably in nanoparticulate form. MTT assays revealed that non-cytotoxic effects were observed for all the samples extracts. Either the plasma treatment or silver deposition were effective in sterilizing the samples and modifying their surfaces. It was demonstrated in this in vitro study that ADSCs associated with NRL films enriched with propolis should be considered promising for application in the healing of occlusive wounds.

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Section: Elemental Analysis Through Energy Dispersive Spectroscopy (Eds)supporting

confidence: 82%

Natural Rubber Films Incorporated with Red Propolis and Silver Nanoparticles Aimed for Occlusive Dressing Application

et al. 2021

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“…The α-end of the rubber chain is linked with mono- and diphosphate groups associated with phospholipids by H-bonding, whereas the ω-end of the rubber chain is linked with a dimethylallyl group associated with protein by H-bonding . C–H stretching at 3000–3200 cm –1 (assigned to CH 2 and CH 3 ), CC stretching at 1660–1680 cm –1 , and C–C stretching at 1000–975 cm –1 are attributed to the chemical structure of NR (Figure a). ,− In particular, C–H stretching bands related to CH 2 and CH 3 are also assigned to the presence of phospholipidic chains and proteins attached to the surface of the NR particles . However, it is hard to distinguish whether the C–H stretching mode comes from the main polymer (polyisoprene) or proteins/phospholipids.…”

Section: Resultsmentioning

confidence: 99%

“…This phase was dried for 24 h at 45 °C and dissolved in chloroform or toluene for 72 h to produce 10 mg/mL NR suspensions. The experimental protocol described here for the preparation of the NR suspensions used in the production NR-SiPs coatings have already been established by our group in previous publications …”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the imaging approach enabled us to investigate the stability of NR-based coatings to advance their structural and morphological characterization for technological applications that require resistance/stability under fluid flow. Furthermore, the core of these fluorescent SiPs used in this work can be modified to produce NR-based coatings with antimicrobial and osteogenic properties acting as a versatile platform for biomedical applications. , …”

Section: Introductionmentioning

confidence: 99%

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Silica Nanoparticles Modified with Fluorescent Dyes as Probes for in Situ Characterization of Natural Rubber Coatings

Oliveira

Paula

Maia

et al. 2022

ACS Appl. Nano Mater.

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In this study, we used fluorescent silica particles (SiPs) as probes to characterize the chemical stability of natural rubber (NR)-based coatings in situ without extensive sample preparation. By tuning the size, concentration, and surface charge of the SiPs and the type of solvent, we obtained two distinct NR-SiPs coatings: (1) irregular honeycomb patterns and (2) flat surfaces. Raman spectral mapping was used to investigate the chemical structure and to illustrate the spatial heterogeneity of the multiple components of the NR-SiPs coatings. The stability of the NR-SiPs coatings was assessed under fluid shear force stress and at high ionic strength conditions (NaCl, 0.85% w/v) using a perfusion chamber coupled with a confocal laser scanning microscope. By evaluating the fluorescent signal of the SiPs, we determined their distribution in the NR-based coatings and monitored their chemical stability via in situ confocal imaging. Using the fluorescent SiPs as probes, we could infer the high chemical stability of the coatings at high ionic strength and under shear stress conditions, opening new horizons for the application of NR coatings in medicine, microelectronics, and the automobile industry.

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“…On the other hand, there is a current lack of use and evaluation of appropriate biomaterials with controlled mechanical and associated interfacial properties, which limits the understanding of the mechanisms of cellular transduction . To the best of our knowledge, no previous study (experimental or theoretical) has been reported that describes and/or predicts favorable cell interactions under various stress–strain conditions connecting the effects of SW. Herein, we propose to use a flexible composite of natural rubber and calcium phosphate (NR-CaP) to fill this gap, motivated by the expected relevance to biomedical science and engineering. − The possibility of using this material in medical applications and in fundamental studies of cell–substrate interactions has stimulated a multidisciplinary interest. Since NR surface properties can be affected by external factors, , the molecular rearrangement of the polymer matrices under mechanical stretch is bound to affect the SW and thereby their interaction with cells.…”

Section: Introductionmentioning

confidence: 99%

Surface Wettability of a Natural Rubber Composite under Stretching: A Model to Predict Cell Survival

et al. 2021

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We report the stress–strain effect of a stretchable natural rubber (NR)–calcium phosphate composite on the surface wettability (SW) using an innovative approach coupling a uniaxial tensile micromachine, goniometer, and microscope. In situ contact angle measurements in real time were performed during mechanical tension. Our results show that SW is guided by the stress–strain relationship with two different characteristics, depending on the static or dynamic experiments. The results evidenced the limits of the classical theory of wetting. Furthermore, based on the mechanically tunable SW of the system associated with the cytocompatibility of the NR composite, we have modeled such a system for application as a cell support. From the experimental surface energy value, our proposed 3D modeling numerical simulation predicted a window of opportunities for cell–NR survival under mechanical stimuli. The presented data and the thermodynamics-based theoretical approach enable not only accurate correlation of SW with mechanical properties of the NR composite but also provide huge potential for future cell supportability in view of tissue engineering.

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Towards the production of natural rubber-calcium phosphate hybrid for applications as bioactive coatings

Cited by 10 publications

References 34 publications

Natural Rubber Films Incorporated with Red Propolis and Silver Nanoparticles Aimed for Occlusive Dressing Application

Natural Rubber Films Incorporated with Red Propolis and Silver Nanoparticles Aimed for Occlusive Dressing Application

Silica Nanoparticles Modified with Fluorescent Dyes as Probes for in Situ Characterization of Natural Rubber Coatings

Surface Wettability of a Natural Rubber Composite under Stretching: A Model to Predict Cell Survival

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