Towards the Expression of Sex Hormone Receptors in the Human Vocal Fold

Schneider, B.; Cohen, Eleonore; Stani, Josefine; Kolbus, Andrea; Rudas, M.; Horvat, Reinhard; Trotsenburg, Mick van

doi:10.1016/j.jvoice.2006.01.002

Cited by 47 publications

(34 citation statements)

References 17 publications

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“…Thus, we have to conclude that nuclear sex hormone receptors in the larynx are not detectable by immunohistochemistry. These data are in accordance with the immunohistochemical results we gathered in vocal fold biopsies taken postmortem [14] . Voelter et al [17] have very recently reported on the investigation of hormone receptors in human vocal fold specimens.…”

Section: Discussionsupporting

confidence: 92%

“…The samples were first analyzed histopathologically. The remaining tissue samples underwent the standard immunohistochemical staining procedure for ER-␣ , PR and AR as described recently [14] : 4 m-thick sections were cut and placed in a 65 ° C stove for 15 min followed by deparaffinization in xylene and rehydration in graded ethanol (ranging from 100 to 70%). Endogenous peroxidase was blocked by 10 min incubation in 3% hydrogen peroxide in methanol.…”

Section: Methodsmentioning

confidence: 99%

“…Whether or not hormone receptors are present in the human vocal fold has been discussed controversially. In a recently published study we investigated the expression of estrogen-␣ receptors (ER-␣ ), progesterone receptors (PR) and androgen receptors (AR) in human vocal folds by immunohistochemistry in cadaver specimens taken approximately 4-8 h postmortem [14] . We could not detect expression of the analyzed hormone receptors.…”

mentioning

confidence: 99%

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Immunohistochemical Examinations of Sex Hormone Receptors in Benign Vocal Fold Lesions

Cohen¹,

Kolbus

Trotsenburg

et al. 2009

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Background: Acquired benign vocal fold lesions are among the most common causes of voice problems. Since the local impact of estrogen and progesterone receptors in laryngeal tissue is discussed controversially, the presence of sex hormone receptors in benign vocal fold alterations needs to be clarified. Goal of the Study: To investigate the expression of estrogen-α receptors (ER-α), estrogen-β receptors (ER-β), progesterone receptors (PR) and androgen receptors (AR) in acquired benign vocal fold alterations. Methods: Laryngeal epithelial specimens of 14 patients (13 female, 1 male) taken intraoperatively were investigated using immunohistochemistry in order to objectify ER-α, ER-β, PR and AR. Macroscopically and histopathologically diagnosed edemas of Reinke’s space (n = 10), vocal fold polyps (n = 3) and vocal fold nodules (n = 1) were enrolled in this study. Results: No specific nuclear immunohistochemical staining could be seen in the biopsies taken. Only unspecific staining patterns could be observed. Conclusion: Sex hormone receptors could not be detected in the specimens tested, thus, any direct influence of sex hormones on the development of benign vocal fold lesions is rather unlikely. The results of this study confirm the impact of vocal fold stress and biomechanical abnormalities on their development due to voice overstraining and abuse.

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunohistochemical Examinations of Sex Hormone Receptors in Benign Vocal Fold Lesions

Cohen¹,

Kolbus

Trotsenburg

et al. 2009

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“…The few studies devoted to the research of sex hormone receptors in normal human vocal cords using enzymatic and immunohistochemical methods are mainly those carried out by Ferguson et al [10] , Marsigliante et al [11] , Newman et al [12] and Schneider et al [13] , who investigated estrogen (ER-␣ ), progesterone (PR) and androgen (AR) receptors in the normal larynx of cadavers (some of whom had died while still fertile and others who had no longer been fertile). Schneider et al [13] also used specimens of normal laryngeal tissue taken from live subjects submitted to laryngectomy for cancer that saved a portion of the vocal cords. Control tissues were generally breast (for ER and PR) and prostate (for AR) specimens [13] .…”

Section: Sex Hormone Receptors In Vocal Foldmentioning

confidence: 99%

“…Schneider et al [13] also used specimens of normal laryngeal tissue taken from live subjects submitted to laryngectomy for cancer that saved a portion of the vocal cords. Control tissues were generally breast (for ER and PR) and prostate (for AR) specimens [13] . In the case of ER-␣ , Ferguson et al [10] and Marsigliante et al [11] reported a positive reaction in the vocalis muscle, the lamina propria and the mesenchyme.…”

Section: Sex Hormone Receptors In Vocal Foldmentioning

confidence: 99%

Sex Hormone Receptors in Vocal Fold Tissue: A Theory about the Influence of Sex Hormones in the Larynx

Nacci¹,

Fattori²,

Basolo

et al. 2010

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Objective: The larynx is considered a secondary sexual organ. To demonstrate that sex hormones can directly influence laryngeal function, specific receptors in the vocal cord must be identified. Materials and Methods: We searched for estrogen, progesterone and androgen receptors, using an immunohistochemical method, in normal human vocal cords (from 3 cadavers) and in samples of healthy vocal cords and of laryngeal carcinomas from 15 live subjects. Breast and prostate carcinoma were used as controls. Results: In all the normal samples tested, the results were negative; there was only a nonspecific cytoplasmatic response in the subepithelial glands (false positives). In the neoplastic tissue, 2 samples had a weak nuclear focal positivity for estrogen and progesterone receptors; all 15 subjects studied were negative for androgen receptors. Conclusions: Since our data show that sex hormone receptors are absent in the vocal cords, other theories must be considered to explain the fact that hormones influence the quality of the voice. This study discusses the possibility that the changes of voice according to gender and throughout life might be linked with a different expression of some growth factors in the laryngeal tissue and that this expression might in turn be influenced by hormonal variations.

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