Towards the development of a sustainable soya bean‐based feedstock for aquaculture

Park, Hyun-Woo; Weier, Steven A.; Razvi, Fareha; Pena, Pamela A.; Sims, Neil Anthony; Lowell, Jennica; Hungate, Cory; Kissinger, Karma R.; Key, Gavin; Fraser, Paul D.; Napier, Johnathan A.; Cahoon, Edgar B.; Clemente, Tom

doi:10.1111/pbi.12608

Cited by 25 publications

(16 citation statements)

References 57 publications

(67 reference statements)

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“…We applied these construction principles to the astaxanthin pathway ( Figure 1A). Astaxanthin (3,3 0 -dihydroxy-b,b-carotene-4,4'-dione) is a high-value ketocarotenoid that is best known for its large-scale commercial use in fish farming to provide the red color to salmon and trout [16]. In addition, it is used industrially as colorant and food supplement, and, due to its extraordinary antioxidant activity, as a pharmaceutical to stimulate immune function and support cancer prevention [17].…”

Section: Resultsmentioning

confidence: 99%

Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species

Stegemann

Agrawal

et al. 2017

Current Biology

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Transgene expression from the plastid (chloroplast) genome provides unique advantages, including high levels of foreign protein accumulation, convenient transgene stacking in operons, and increased biosafety due to exclusion of plastids from pollen transmission [1, 2]. However, applications in biotechnology and synthetic biology are severely restricted by the very small number of plant species whose plastid genomes currently can be transformed [3, 4]. Here we report a simple method for the introduction of useful plastid transgenes into non-transformable species. The transgenes tested comprised a synthetic operon encoding three components of a biosynthetic pathway for producing the high-value ketocarotenoid astaxanthin in the plastids of the cigarette tobacco, Nicotiana tabacum. Transplastomic N. tabacum plants accumulated astaxanthin to up to 1% of the plants' dry weight. We then used grafting, a procedure recently shown to facilitate horizontal genome transfer between plants [5-7], to let the transgenic chloroplast genome move across the graft junction from N. tabacum plants into plants of the nicotine-free tree species Nicotiana glauca. Transplastomic N. glauca trees expressing the synthetic pathway were recovered at high frequency, thus providing a straightforward method for extension of the transplastomic technology to new species.

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Section: Resultsmentioning

confidence: 99%

Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species

Stegemann

Agrawal

et al. 2017

Current Biology

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“…Recently, a multi-gene stack approach was used to develop what could potentially make a sustainable soybean-based feedstock for aquaculture. (Park et al 2016 ). In this study, they improved EPA (eicosapentaenoic acid) and astaxanthin concentrations resulting in a novel oil trait without affecting protein quality.…”

Section: Genetic Analyses Of Protein Contentmentioning

confidence: 99%

Molecular mapping and genomics of soybean seed protein: a review and perspective for the future

et al. 2017

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Key message Genetic improvement of soybean protein meal is a complex process because of negative correlation with oil, yield, and temperature. This review describes the progress in mapping and genomics, identifies knowledge gaps, and highlights the need of integrated approaches. AbstractMeal protein derived from soybean [Glycine max (L) Merr.] seed is the primary source of protein in poultry and livestock feed. Protein is a key factor that determines the nutritional and economical value of soybean. Genetic improvement of soybean seed protein content is highly desirable, and major quantitative trait loci (QTL) for soybean protein have been detected and repeatedly mapped on chromosomes (Chr.) 20 (LG-I), and 15 (LG-E). However, practical breeding progress is challenging because of seed protein content’s negative genetic correlation with seed yield, other seed components such as oil and sucrose, and interaction with environmental effects such as temperature during seed development. In this review, we discuss rate-limiting factors related to soybean protein content and nutritional quality, and potential control factors regulating seed storage protein. In addition, we describe advances in next-generation sequencing technologies for precise detection of natural variants and their integration with conventional and high-throughput genotyping technologies. A syntenic analysis of QTL on Chr. 15 and 20 was performed. Finally, we discuss comprehensive approaches for integrating protein and amino acid QTL, genome-wide association studies, whole-genome resequencing, and transcriptome data to accelerate identification of genomic hot spots for allele introgression and soybean meal protein improvement.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-017-2955-8) contains supplementary material, which is available to authorized users.

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“…The biochemical basis for this approach is that increased activity of phytoene synthase in plastids directs enhanced flux of geranylgeranyl diphosphate (GGDP) to the biosynthesis of carotenoids. Expression of phytoene synthase alone or in combination with enzymes such as the bacterial phytoene desaturases has been used to generate substantial increases in amounts of carotenoids in a number of plants including canola (Shewmaker et al, 1999), rice (Paine et al, 2005;Ye et al, 2000), sorghum (Che et al, 2016;Lipkie et al, 2013), tomato (Fraser et al, 2002;Romer et al, 2000), potato (Diretto et al, 2007), soya bean (Park et al, 2017;Schmidt et al, 2015) and banana (Paul et al, 2017). Additional approaches have included the expression of plastid isoprenoid pathway enzymes.…”

Section: Introductionmentioning

confidence: 99%

Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch

Beyene

Solomon

Chauhan

et al. 2017

Plant Biotechnology Journal

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SummaryStorage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub‐Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β‐carotene. In this study, β‐carotene concentrations in cassava storage roots were enhanced by co‐expression of transgenes for deoxy‐d‐xylulose‐5‐phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin‐type 1 promoter. Storage roots harvested from field‐grown plants accumulated carotenoids to ≤50 μg/g DW, 15‐ to 20‐fold increases relative to roots from nontransgenic plants. Approximately 85%–90% of these carotenoids accumulated as all‐trans‐β‐carotene, the most nutritionally efficacious carotenoid. β‐Carotene‐accumulating storage roots displayed delayed onset of postharvest physiological deterioration, a major constraint limiting utilization of cassava products. Large metabolite changes were detected in β‐carotene‐enhanced storage roots. Most significantly, an inverse correlation was observed between β‐carotene and dry matter content, with reductions of 50%–60% of dry matter content in the highest carotenoid‐accumulating storage roots of different cultivars. Further analysis confirmed a concomitant reduction in starch content and increased levels of total fatty acids, triacylglycerols, soluble sugars and abscisic acid. Potato engineered to co‐express DXS and crtB displayed a similar correlation between β‐carotene accumulation, reduced dry matter and starch content and elevated oil and soluble sugars in tubers. Transcriptome analyses revealed a reduced expression of genes involved in starch biosynthesis including ADP‐glucose pyrophosphorylase genes in transgenic, carotene‐accumulating cassava roots relative to nontransgenic roots. These findings highlight unintended metabolic consequences of provitamin A biofortification of starch‐rich organs and point to strategies for redirecting metabolic flux to restore starch production.

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Towards the development of a sustainable soya bean‐based feedstock for aquaculture

Cited by 25 publications

References 57 publications

Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species

Horizontal Transfer of a Synthetic Metabolic Pathway between Plant Species

Molecular mapping and genomics of soybean seed protein: a review and perspective for the future

Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch

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