Towards Synthetic PETtrophy: EngineeringPseudomonas putidafor concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression
Abstract:BackgroundBiocatalysis offers a promising path for plastic waste management and valorization, especially for hydrolysable plastics such as polyethylene terephthalate (PET). Microbial whole-cell biocatalysts for simultaneous PET degradation and growth on PET monomers would offer a one-step solution toward PET recycling or upcycling. We set out to engineer the industry-proven bacterium Pseudomonas putida for (i) metabolism of PET monomers as sole carbon sources, and (ii) efficient concurrent extracellular expres… Show more
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