Towards standardization of electroporation devices and protocols

Cvetkoska, Aleksandra; Piro, Eva; Reberšek, Matej; Magjarević, Ratko; Miklavčič, Damijan

doi:10.1109/mim.2020.9062692

Cited by 16 publications

(7 citation statements)

References 17 publications

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“…According to the European Standard Operating Procedures of Electrochemotherapy (ESOPE) for subcutaneous tumors, pulsed electric field must be generated in deeper tissues; hence, invasive electrodes are required. In contrast, non-invasive electrodes are frequently used for cutaneous targets and have limited applicability for deep-seated tumors ( Cvetkoska et al, 2020 ). Electroporation-based treatment success depends on the coverage of the tumor tissue by sufficiently high local electric field, requiring good contact area between the tissue and the electrodes ( Čorović et al, 2008 ; Sachdev et al, 2022 ).…”

Section: Mammalian Tissue Electroporationmentioning

confidence: 99%

Invasive and non-invasive electrodes for successful drug and gene delivery in electroporation-based treatments

Malyško-Ptašinskė

Staigvila

Novickij

2023

Front. Bioeng. Biotechnol.

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Electroporation is an effective physical method for irreversible or reversible permeabilization of plasma membranes of biological cells and is typically used for tissue ablation or targeted drug/DNA delivery into living cells. In the context of cancer treatment, full recovery from an electroporation-based procedure is frequently dependent on the spatial distribution/homogeneity of the electric field in the tissue; therefore, the structure of electrodes/applicators plays an important role. This review focuses on the analysis of electrodes and in silico models used for electroporation in cancer treatment and gene therapy. We have reviewed various invasive and non-invasive electrodes; analyzed the spatial electric field distribution using finite element method analysis; evaluated parametric compatibility, and the pros and cons of application; and summarized options for improvement. Additionally, this review highlights the importance of tissue bioimpedance for accurate treatment planning using numerical modeling and the effects of pulse frequency on tissue conductivity and relative permittivity values.

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Section: Mammalian Tissue Electroporationmentioning

confidence: 99%

Invasive and non-invasive electrodes for successful drug and gene delivery in electroporation-based treatments

Malyško-Ptašinskė

Staigvila

Novickij

2023

Front. Bioeng. Biotechnol.

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“…The bigger the capacitor, the smaller the droop; however, it is always a trade-off between the size of the electroporator, its price, and pulsing flexibility. Nowadays, a droop of voltage up to 10%–20% is usually tolerated in electroporation studies ( 23 ), while it should be understood that 10% is still a significant change in the electric field for a threshold-type phenomenon like electroporation.…”

Section: The First Waveforms For Electroporationmentioning

confidence: 99%

Does the shape of the electric pulse matter in electroporation?

et al. 2022

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Electric pulses are widely used in biology, medicine, industry, and food processing. Numerous studies indicate that electroporation (EP) is a pulse-dependent process, and the electric pulse shape and duration strongly determine permeabilization efficacy. EP protocols are precisely planned in terms of the size and charge of the molecules, which will be delivered to the cell. In reversible and irreversible EP applications, rectangular or sine, polar or bipolar pulses are commonly used. The usage of pulses of the asymmetric shape is still limited to high voltage and low voltage (HV/LV) sequences in the context of gene delivery, while EP-based applications of ultra-short asymmetric pulses are just starting to emerge. This review emphasizes the importance and role of the pulse shape for membrane permeabilization by EP.

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“…Electroporation and microinjection of the transgene into cells through physical methods of transfection provide safer alternatives to viral vectors. But these methods require specialized equipment, which limits their use and accessibility (Cvetkoska et al, 2020). Nonviral agents have emerged as a better alternative to viral transduction and physical methods as a cheap and efficient gene delivery method both in vitro and in vivo (Al‐Dosari & Gao, 2009).…”

Section: Introductionmentioning

confidence: 99%

Anhydride chemistry‐based hexanoylation of polyethylenimine increases transfection efficiency and expression of tagged DNA for therapeutic proteins in cultured cells

Ulgekar

Kaur

Ganesan

et al. 2022

Biotech & Bioengineering

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Transfection of nucleic acid molecules into mammalian cells can be facilitated using viral vectors, electroporation, or biocompatible cationic materials. However, safety issues and the requirement of specialized equipment limits the use of viral vectors and physical methods of transfection like electroporation and microinjection, respectively. Biocompatible cationic lipids and polymers like branched‐polyethyleneimine (bPEI) have a wide transfection range and are user‐friendly in most applications. However, bPEI exhibits low transfection efficiency in most cell types. In the present work, we have crosslinked the hexanoyl group to bPEI using anhydride chemistry to enhance its efficiency as a transfection reagent. The efficient association of hexanoyl group to bPEI was assessed using Fourier‐transform infrared spectroscopy and other physicochemical methods. Hexanoyl‐modified bPEI (FA6‐bPEI) was found to exhibit significantly enhanced transfection efficiency in both cell lines and cultured primary cells, as compared to native bPEI and the commercially available transfection reagent, Lipofectamine 3000. Furthermore, our in vitro studies indicated that FA6‐bPEI can be used for robust transfection for increased production of therapeutic proteins in a cell culture‐based system. These results suggested that hexanoyl‐modified bPEI can serve as an efficient transfection reagent for studies on hard‐to‐transfect cells and for enhanced production of therapeutic proteins in vitro.

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Towards standardization of electroporation devices and protocols

Cited by 16 publications

References 17 publications

Invasive and non-invasive electrodes for successful drug and gene delivery in electroporation-based treatments

Invasive and non-invasive electrodes for successful drug and gene delivery in electroporation-based treatments

Does the shape of the electric pulse matter in electroporation?

Anhydride chemistry‐based hexanoylation of polyethylenimine increases transfection efficiency and expression of tagged DNA for therapeutic proteins in cultured cells

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