Towards solving the mystery of peroxisomal matrix protein import

Skowyra, Michael L.; Feng, Peiqiang; Rapoport, Tom A.

doi:10.1016/j.tcb.2023.08.005

Cited by 3 publications

(1 citation statement)

References 133 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent data on the membrane topology of PEX13 suggest that at least half of the PEX13 molecules present at the peroxisomal membrane direct their N-termini towards the cytosol. 26,51 In addition, considering a recent model for the architecture of the docking/translocation module in which the flexible N-terminal domain of PEX13 rests at the center of the protein translocation pore, 6,26 it is possible that the PEX13 KPWE motif interacts with the cargo-loaded PEX7-coreceptor complex to facilitate cargo translocation.…”

Section: Discussionmentioning

confidence: 99%

PEX39 facilitates the peroxisomal import of PTS2 proteins

Chen,

Rodrigues,

Wendscheck

et al. 2024

Preprint

View full text Add to dashboard Cite

Peroxisomes are metabolic organelles essential for human health. Defects in peroxisomal biogenesis proteins (peroxins/PEXs) cause devastating disease. PEX7 binds newly synthesized proteins containing a type 2 peroxisomal targeting signal (PTS2) to enable their import from the cytosol into peroxisomes, although many aspects of this import pathway remain enigmatic. Utilizing in vitro assays, yeast, and human cells, we show that PEX39, a previously uncharacterized protein, is a cytosolic peroxin that facilitates PTS2-protein import by binding PEX7 and stabilizing its interaction with PTS2 cargo. PEX39 and PEX13, a peroxisomal membrane translocon protein, both possess a KPWE motif necessary for PEX7 binding. Sequential binding of PEX7 to this motif in PEX39 and PEX13 provides a novel paradigm for how PTS2 cargo engage the translocation machinery. Collectively, our work uncovers an ancient and functionally important relationship among PEX39, PEX7, and PEX13, offering insights that will advance our understanding of peroxisomal biogenesis and disease.

show abstract

Section: Discussionmentioning

confidence: 99%

PEX39 facilitates the peroxisomal import of PTS2 proteins

Chen,

Rodrigues,

Wendscheck

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Fluorescence Methods to Measure Pexophagy

Barone,

Urbé,

Clague

2024

Methods in Molecular Biology

View full text Add to dashboard Cite

The Key Targets of NO-Mediated Post-Translation Modification (PTM) Highlighting the Dynamic Metabolism of ROS and RNS in Peroxisomes

Ergashev,

Yu,

Luo

et al. 2024

IJMS

View full text Add to dashboard Cite

Nitric oxide (NO) has been firmly established as a key signaling molecule in plants, playing a significant role in regulating growth, development and stress responses. Given the imperative of sustainable agriculture and the urgent need to meet the escalating global demand for food, it is imperative to safeguard crop plants from the effects of climate fluctuations. Plants respond to environmental challenges by producing redox molecules, including reactive oxygen species (ROS) and reactive nitrogen species (RNS), which regulate cellular, physiological, and molecular processes. Nitric oxide (NO) plays a crucial role in plant stress tolerance, acting as a signaling molecule or free radical. NO is involved in various developmental processes in plants through diverse mechanisms. Exogenous NO supplementation can alleviate the toxicity of abiotic stresses and enhance plant resistance. In this review we summarize the studies regarding the production of NO in peroxisomes, and how its molecule and its derived products, (ONOO−) and S-nitrosoglutathione (GSNO) affect ROS metabolism in peroxisomes. Peroxisomal antioxidant enzymes including catalase (CAT), are key targets of NO-mediated post-translational modification (PTM) highlighting the dynamic metabolism of ROS and RNS in peroxisomes.

show abstract

Towards solving the mystery of peroxisomal matrix protein import

Cited by 3 publications

References 133 publications

PEX39 facilitates the peroxisomal import of PTS2 proteins

PEX39 facilitates the peroxisomal import of PTS2 proteins

Fluorescence Methods to Measure Pexophagy

The Key Targets of NO-Mediated Post-Translation Modification (PTM) Highlighting the Dynamic Metabolism of ROS and RNS in Peroxisomes

Contact Info

Product

Resources

About