Towards <scp>dsRNA</scp>‐integrated protection of medical <i>Cannabis</i> crops: considering human safety, recent‐ and developing <scp>RNAi</scp> methods, and research inroads

Willow, Jonathan; Silva, Ana Isabel; Taning, Clauvis Nji Tizi; Smagghe, Guy; Veromann, Eve

doi:10.1002/ps.7323

Cited by 2 publications

(1 citation statement)

References 84 publications

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“…SRBSDV transmission requires coat protein or non-structural proteins to bind to the insect receptors to spread, thus, disrupting the virus target receptor in the vector is one of the effective means to control its transmission. However, many areas still need to be addressed, including (a) the large-scale application of dsRNA-mediated RNAi-based pest management approaches, 125,142 (b) the impact of epigenetic methylation on the interactions between SRBSDV and S. furcifera, and (c) the impact of the gut microbiota of S. furcifera on the transmission of SRBSDV. Second, genetic resistance to plant virus diseases is the most economical and environmentally friendly control strategy.…”

Section: Perspectivesmentioning

confidence: 99%

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

Luo

Zhang

et al. 2023

Pest Management Science

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Southern rice black‐streaked dwarf virus (SRBSDV) is one of the most damaging rice viruses. The virus decreases rice quality and yield, and poses a serious threat to food security. From this perspective, this review performed a survey of published studies in recent years to understand the current status of SRBSDV and white‐backed planthopper (WBPH, Sogatella furcifera) transmission processes in rice. Recent studies have shown that the interactions between viral virulence proteins and rice susceptibility factors shape the transmission of SRBSDV. Moreover, the transmission of SRBSDV is influenced by the interactions between viral virulence proteins and S. furcifera susceptibility factors. This review focused on the molecular mechanisms of key genes or proteins associated with SRBSDV infection in rice via the S. furcifera vector, and the host defense response mechanisms against viral infection. A sustainable control strategy using RNAi was summarized to address this pest. Finally, we also present a model for screening anti‐SRBSDV inhibitors using viral proteins as targets. © 2023 Society of Chemical Industry.

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Section: Perspectivesmentioning

confidence: 99%

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

Luo

Zhang

et al. 2023

Pest Management Science

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Biosafety and toxicity assessment of transgenic cotton-harboring insecticide and herbicide tolerant genes on albino mice

Bhutta,

Awais,

Raouf

et al. 2024

Toxicology Research

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Introduction Genetic engineering has revolutionized agriculture by transforming biotic and abiotic stress-resistance genes in plants. The biosafety of GM crops is a major concern for consumers and regulatory authorities. Methodology A 14-week biosafety and toxicity analysis of transgenic cotton, containing 5 transgenes ((Cry1Ac, Cry2A, CP4 EPSPS, VIP3Aa, and ASAL)), was conducted on albino mice. Thirty mice were divided into three groups (Conventional, Non-transgenic, without Bt, and transgenic, containing targeted crop) according to the feed given, with 10 mice in each group, with 5 male and 5 female mice in each group. Results During the study, no biologically significant changes were observed in the non-transgenic and transgenic groups compared to the control group in any of the study’s parameters i.e. increase in weight of mice, physiological, pathological, and molecular analysis, irrespective of the gender of the mice. However, a statistically significant change was observed in the hematological parameters of the male mice, while no such change was observed in the female study group mice. The expression analysis, however, of the TNF gene increases many folds in the transgenic group as compared to the non-transgenic and conventional groups. Conclusion Overall, no physiological, pathological, or molecular toxicity was observed in the mice fed with transgenic feed. Therefore, it can be speculated that the targeted transgenic crop is biologically safe. However, more study is required to confirm the biosafety of the product on the animal by expression profiling.

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Towards dsRNA‐integrated protection of medical Cannabis crops: considering human safety, recent‐ and developing RNAi methods, and research inroads

Cited by 2 publications

References 84 publications

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

A systematic review of southern rice black‐streaked dwarf virus in the age of omics

Biosafety and toxicity assessment of transgenic cotton-harboring insecticide and herbicide tolerant genes on albino mice

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