Towards real-time observation of conditioning film and early biofilm formation under laminar flow conditions using a quartz crystal microbalance

Chen, Mingyang; Chen, Ming‐Jen; Lee, Pei-Fang; Cheng, Lin-Wen; Huang, Li-Jen; Lai, Chin-Hsing; Huang, Kuei-Hsiang

doi:10.1016/j.bej.2010.10.003

Cited by 26 publications

(15 citation statements)

References 42 publications

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“…Earlier studies on surfaces exposed to seawater have identified carbohydrates and proteins as the initial and most abundant constituents of the primary conditioning film (Comp ere et al 2001;Garg et al 2009;Jain and Bhosle 2009). Likewise, our finding of an immediate onset of the conditioning film formation after exposure of the wafer surface to the aquifer water is in agreement with earlier observations on surfaces immersed in sea water (Bakker et al 2003) and conditioning films produced from artificial growth media (Chen et al 2010).…”

Section: Discussionsupporting

confidence: 92%

An Imaging Mass Spectrometry Study on the Formation of Conditioning Films and Biofilms in the Subsurface (Äspö Hard Rock Laboratory, SE Sweden)

Leefmann

Heim

Lausmaa

et al. 2015

Geomicrobiology Journal

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Conditioning films and biofilms forming on surfaces of solid materials exposed to aqueous media play a key role in in the interaction between the geo-and biospheres. In this study, time-of-flight secondary ion mass spectrometry and scanning electron microscopy were used to investigate the time scale, mode of formation, and chemistry of conditioning films and biofilms that formed on Si substrates exposed to aquifer water in the subsurface € Asp€ o Hard Rock Laboratory, SE-Sweden. The detection of fragment ions of amino acids, carbohydrates, and carboxylic acids revealed that different types of organic compounds had adhered to the Si surface already after 10 min of exposure to the aquifer fluids, whereas the attachment of microbial cells was first observed after 1000 min. The organic compounds first formed isolated mm-sized accumulations and subsequently started to distribute on the wafer surface more homogenously. Simultaneously further microorganisms attached to the surface and formed biofilm-like cell accumulations after 3 months of exposure to aquifer water.

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Section: Discussionsupporting

confidence: 92%

An Imaging Mass Spectrometry Study on the Formation of Conditioning Films and Biofilms in the Subsurface (Äspö Hard Rock Laboratory, SE Sweden)

Leefmann

Heim

Lausmaa

et al. 2015

Geomicrobiology Journal

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“…Cells of L. monocytogenes within biofilms are more resistant to biocides (102), which increases the risk of food contamination. Initial adherence is critical for biofilm formation and depends on the physiochemical properties of the environmental surfaces as well as the biofilm-forming potential of the bacterial cells (18,83,102). L. monocytogenes can adhere to abiotic surfaces such as stainless steel, glass, plastic, polymers, and rubber that are present in the food processing environment (44,102,107).…”

mentioning

confidence: 99%

comK Prophage Junction Fragments as Markers for Listeria monocytogenes Genotypes Unique to Individual Meat and Poultry Processing Plants and a Model for Rapid Niche-Specific Adaptation, Biofilm Formation, and Persistence

Verghese

Lok

Wen

et al. 2011

Appl Environ Microbiol

119

112

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Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as "adaptons," as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety.Listeria monocytogenes is a unique food-borne pathogen that causes listeriosis, which ranges from febrile gastroenteritis to more severe life-threatening invasive diseases, especially for immunocompromised individuals (94). It is widely distributed in many wild and domestic animals and various natural environments and is resistant to a wide variety of environmental stresses (33). L. monocytogenes is considered a model organism for the study of host-pathogen interactions, especially as a model for intracellular pathogens of humans (47). However, L. monocytogenes may also be an excellent model for pathogenenvironment interactions, because it is well known to cycle between being a pathogen in many wild and domestic animals and a saprophyte in diverse environments, including those found in various types of food processing facilities (41). However, while much is known about the pathogenic lifestyle of L. monocytogenes, much less is known about its saprophytic lifestyle, including the genetic and phenotypic factors affecting its colonization and persistence in food processing and retail environments and subsequent transmission to ready-to-eat (RTE) foods. This has resulted in numerous costly recalls, disease cases, and outbreaks, which are often associated with high mortality (94). As a res...

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“…However, the accumulation of the conditioning film continues for hours after first exposure [47], [48]. The formation of a conditioning film on the biomaterials surfaces during the lag-phase is most likely [48].…”

Section: Discussionmentioning

confidence: 99%

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

et al. 2014

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Biomaterials-associated infections are primarily initiated by the adhesion of microorganisms on the biomaterial surfaces and subsequent biofilm formation. Understanding the fundamental microbial adhesion mechanisms and biofilm development is crucial for developing strategies to prevent such infections. Suitable in vitro systems for biofilm cultivation and bacterial adhesion at controllable, constant and reproducible conditions are indispensable. This study aimed (i) to modify the previously described constant-depth film fermenter for the reproducible cultivation of biofilms at non-depth-restricted, constant and low shear conditions and (ii) to use this system to elucidate bacterial adhesion kinetics on different biomaterials, focusing on biomaterials surface nanoroughness and hydrophobicity. Chemostat-grown Escherichia coli were used for biofilm cultivation on titanium oxide and investigating bacterial adhesion over time on titanium oxide, poly(styrene), poly(tetrafluoroethylene) and glass. Using chemostat-grown microbial cells (single-species continuous culture) minimized variations between the biofilms cultivated during different experimental runs. Bacterial adhesion on biomaterials comprised an initial lag-phase I followed by a fast adhesion phase II and a phase of saturation III. With increasing biomaterials surface nanoroughness and increasing hydrophobicity, adhesion rates increased during phases I and II. The influence of materials surface hydrophobicity seemed to exceed that of nanoroughness during the lag-phase I, whereas it was vice versa during adhesion phase II. This study introduces the non-constant-depth film fermenter in combination with a chemostat culture to allow for a controlled approach to reproducibly cultivate biofilms and to investigate bacterial adhesion kinetics at constant and low shear conditions. The findings will support developing and adequate testing of biomaterials surface modifications eventually preventing biomaterial-associated infections.

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Towards real-time observation of conditioning film and early biofilm formation under laminar flow conditions using a quartz crystal microbalance

Cited by 26 publications

References 42 publications

An Imaging Mass Spectrometry Study on the Formation of Conditioning Films and Biofilms in the Subsurface (Äspö Hard Rock Laboratory, SE Sweden)

An Imaging Mass Spectrometry Study on the Formation of Conditioning Films and Biofilms in the Subsurface (Äspö Hard Rock Laboratory, SE Sweden)

comK Prophage Junction Fragments as Markers for Listeria monocytogenes Genotypes Unique to Individual Meat and Poultry Processing Plants and a Model for Rapid Niche-Specific Adaptation, Biofilm Formation, and Persistence

Reproducible Biofilm Cultivation of Chemostat-Grown Escherichia coli and Investigation of Bacterial Adhesion on Biomaterials Using a Non-Constant-Depth Film Fermenter

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