Towards proteomic analysis of milk proteins in historical building materials

Kučková, Štěpánka; Crhova, Michaela; Vaňková, Lenka; Hnı́zda, Aleš; Hynek, Radovan; Kodı́ček, Milan

doi:10.1016/j.ijms.2009.01.011

Cited by 46 publications

(31 citation statements)

References 28 publications

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“…It has been applied more recently to the analysis of proteins in ancient bones (102,103) and eggshells (104), where it allows the identification of species, and has been used to analyse proteinaceous and lipid binders in works of art and wall plasters (105)(106)(107)(108). It has been used to analyze proteinaceous glue from a wooden building (109), to study the photo-degradation of terpenoid varnishes (110), and to assess the condition and penetration of polyethylene glycol in preserved ships (111).…”

Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

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The analysis of organic residues from archaeological materials has become increasingly important to our understanding of ancient diet, trade and technology. Residues from diverse contexts have been retrieved and analysed from the remains of food, medicine and cosmetics to hafting material on stone arrowheads, pitch and tar from shipwrecks, and ancient manure from soils. Research has brought many advances in our understanding of archaeological, organic residues over the past two decades. Some have enabled very specific and detailed interpretations of materials preserved in the archaeological record. However there are still areas where we know very little, like the mechanisms at work during the formation and preservation of residues, and areas where each advance produces more questions rather than answers, as in the identification of degraded fats. This chapter will discuss some of the significant achievements in the field over the past decade and the ongoing challenges for research in this area.1341280_File000001_20903114.docPrinted 7/19/2013 2

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Section: New Mass Spectrometric Techniquesmentioning

confidence: 99%

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Steele

2013

ACS Symposium Series

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“…The resulting soluble specific peptide fragments can be easily extracted and subsequently analyzed by mass spectrometry. This method was originally developed for the identification of protein binders in art works [3] and subsequently successfully used for analysis of proteins in insoluble materials like color layers or historical mortars [4][5][6][7][8]. We anticipated that the above-mentioned principle could also be applied to the analysis of proteins in hard tissues.…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Protein Analysis in Hard Tissues

Hynek

Kučková

Koník

et al. 2011

JSA

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This preliminary study is aimed at a new approach in the protein analysis of hard tissues. Analytical methods that have been used for the identification of proteins in bones and teeth always require one critical step -demineralization. This chemical treatment is responsible for loss of proteins and it also negatively influences the possibility of protein quantification. The method of peptide mass mapping described in this paper facilitates a gentle releasing of peptides from the hard tissues without the loss of qualitative and quantitative information. Using this method led to identification of proteins from chicken thighbone, human jawbone and teeth, which proves the feasibility of this method.

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“…Furthermore, the precise quantification of binders in complex mixtures was rarely achieved. Few innovative techniques (MALDI-TOF-MS, immune-enzymatic assays) were reported lately for identification of natural binders, but further research still has to be done [31][32][33][34][35][36][37][38][39][40][41][42][43][44][45].…”

Section: State-of-the-art Of Proteinaceous Binders Characterizationmentioning

confidence: 99%

“…2 [3][4]25]. could make an important contribution to the study of proteinaceous paint media and their degradation processes [31][32][33][34][35][36][37][38]. Some papers lately reported successful attempts for analysis of protein binders using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) [34].…”

Section: Fourier Transform Infrared (Ftir/micro-ftir) and Micro-ramanmentioning

confidence: 99%

The Biochemistry and Artistic studies: a novel integrated approach to the identification of proteinaceous binders in polychrome artifacts

Sandu¹,

Roque²,

Kučková³

et al. 2013

Estudos De Conservação E Restauro

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The paper proposes a novel, integrated approach to the identification and quantification of the organic binders, mainly proteinaceous, as the "invisible" component of polychrome layers in artifacts belonging to movable and immovable cultural heritage. The analytical methodology discussed here will demonstrate the significance and efficiency of biochemical assays for the detection of organic binders and how these tests can improve/complement the already existing techniques, while considering also the costs and time of analysis that commonly limit their application in the current laboratory practice for artworks diagnosis. KeywordsBiochemical analyses, organic binders, identification and quantification, works of art. ResumoEste artigo propõe uma abordagem nova e integrada para a identificação e quantificação aglutinantes orgânicos, na sua maioria proteicos, como componentes invisíveis das camadas policromadas dos objectos que integram o património móvel e imóvel. A discussão da metodologia analítica que aqui se faz, demonstrará a importância da eficácia dos ensaios bioquímicos na detecção de aglutinantes orgânicos e o modo como estes testes podem ser melhorados e complementar as técnicas analíticas disponíveis, tendo em conta os factores custo e tempo de realização das análises, em geral condicionantes que limitam a sua aplicação na prática corrente do trabalho laboratorial de diagnóstico em obras de arte. Palavras-chaveAnálises bioquímicas, aglutinantes orgânicos, identificação e quantificação, obras de arte. ResumenEste trabajo propone una novedosa aproximación para la identificación y cuantificación de los aglutinantes, fundamentalmente a base de proteínas, que forman capas "invisibles" como componentes de la policromía en objetos muebles e inmuebles del patrimonio cultural.La metodología analítica presentada demostrará la eficiencia de los ensayos bioquímicos para la detección de aglutinantes orgánicos y cómo esos tests pueden complementar las 40 estudos de conservação e restauro | nº 1 técnicas ya existentes, incluso considerando también los costes y tiempo necesarios para los análisis que normalmente limitan aquella posible aplicación en el diagnóstico de objetos artísticos en la práctica habitual de laboratorio. Palabras claveAnálisis bioquímicos, aglutinantes orgánicos, identificación y cuantificación, obras de arte.

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Towards proteomic analysis of milk proteins in historical building materials

Cited by 46 publications

References 28 publications

Organic Residues in Archaeology: The Highs and Lows of Recent Research

Organic Residues in Archaeology: The Highs and Lows of Recent Research

A Novel Approach to Protein Analysis in Hard Tissues

The Biochemistry and Artistic studies: a novel integrated approach to the identification of proteinaceous binders in polychrome artifacts

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