Towards practical sample preparation in point-of-care testing: user-friendly microfluidic devices

Park, Ju-Hwan; Han, Dong Hyun; Park, Je‐Kyun

doi:10.1039/d0lc00047g

Cited by 119 publications

(73 citation statements)

References 147 publications

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“…In addition, POCT has other applications, including serum separation, detection of enzyme activity, detection of bacteria, viruses, proteins, DNA, etc. [31]. The methods based on POCT can be divided into self-driving testing and external force-driving testing according to driving force [31].…”

Section: Detection Methods Based On Point-of-care Testing (Poct)mentioning

confidence: 99%

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Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

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Section: Detection Methods Based On Point-of-care Testing (Poct)mentioning

confidence: 99%

“…[31]. The methods based on POCT can be divided into self-driving testing and external force-driving testing according to driving force [31]. Self-driving detection includes the use of capillary effect, negative pressure, or chemical reaction to generate gas [32,130], etc.…”

Section: Detection Methods Based On Point-of-care Testing (Poct)mentioning

confidence: 99%

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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“…It allows the diffusion of a biological sample through a capillary effect, eliminating the need for external power sources. Earlier studies have introduced lateral flow test strips and microfluidic paper-based analytical devices (μPAD) for POC testing [ 22 , 23 ]. The assay usually involves hybridization of single-stranded DNA or RNA with a complementary probe to produce double-stranded nucleic acids or interaction between antigen and antibody to produce an Ag-Ab complex, generating signals such as colorimetric, fluorescence, or chemiluminescence signals [ 81 ].…”

Section: Recent Advances In Poc Technologies For Covid-19mentioning

confidence: 99%

“…Significant efforts have been devoted to the development of point-of-care (POC) biosensors, such as chip-based and paper-based biosensors to substitute the conventional assays for the diagnosis of infectious diseases at the POC [21][22][23]. Due to the high demand for rapid testing to control the current pandemic, POC biosensors which are of low cost and easy to use and have sample-in-answer-out capability are highly desirable [24,25].…”

Section: Introductionmentioning

confidence: 99%

Emerging point-of-care biosensors for rapid diagnosis of COVID-19: current progress, challenges, and future prospects

Rasmi

Khan

et al. 2021

Anal Bioanal Chem

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Coronavirus disease 2019 (COVID-19) pandemic is currently a serious global health threat. While conventional laboratory tests such as quantitative real-time polymerase chain reaction (qPCR), serology tests, and chest computerized tomography (CT) scan allow diagnosis of COVID-19, these tests are time-consuming and laborious, and are limited in resource-limited settings or developing countries. Point-of-care (POC) biosensors such as chip-based and paper-based biosensors are typically rapid, portable, cost-effective, and user-friendly, which can be used for COVID-19 in remote settings. The escalating demand for rapid diagnosis of COVID-19 presents a strong need for a timely and comprehensive review on the POC biosensors for COVID-19 that meet ASSURED criteria: Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverable to end users. In the present review, we discuss the importance of rapid and early diagnosis of COVID-19 and pathogenesis of COVID-19 along with the key diagnostic biomarkers. We critically review the most recent advances in POC biosensors which show great promise for the detection of COVID-19 based on three main categories: chip-based biosensors, paper-based biosensors, and other biosensors. We subsequently discuss the key benefits of these biosensors and their use for the detection of antigen, antibody, and viral nucleic acids. The commercial POC biosensors for COVID-19 are critically compared. Finally, we discuss the key challenges and future perspectives of developing emerging POC biosensors for COVID-19. This review would be very useful for guiding strategies for developing and commercializing rapid POC tests to manage the spread of infections.

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“…Miniaturization of benchtop instruments in form of microfluidic devices offers a great opportunity for the handling, manipulation, preparation and analysis of minute, precious samples using these devices in e.g. point-of-care diagnostics 1 , drug delivery 2 , single cell analysis 3 or PCR tests 4 . Also the fractionation of analyte mixtures as up-stream or down-stream processing in complex workflows can be done in microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

Coupling Miniaturized Free-Flow Electrophoresis to Mass Spectrometry via a Multi-Emitter ESI Interface

Jender¹,

Höving²,

Novo³

et al. 2020

Preprint

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Towards practical sample preparation in point-of-care testing: user-friendly microfluidic devices

Abstract: An overview and future perspectives of user-friendly microfluidic devices have been reported in terms of practical sample preparation in point-of-care testing.

Cited by 119 publications

References 147 publications

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Emerging point-of-care biosensors for rapid diagnosis of COVID-19: current progress, challenges, and future prospects

Coupling Miniaturized Free-Flow Electrophoresis to Mass Spectrometry via a Multi-Emitter ESI Interface

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