Towards Opto-Magnetic Physiology: Concepts and Pitfalls of ofMRI

Beyond correlation: functional OPTO-MAgnetic Integration Concept (OPTOMAIC) to reveal the brain-wide signature of local neuronal signals-of-interest

“…Yet, certainly, it cannot be avoided that a rupture of a hidden blood vessel occurs, in which case the respective experiment cannot be continued (please refer also to Refs. 5 and 38 ).…”

“…After optical fiber implantation, the signal quality of the calcium signal must be probed by a test measurement and, if necessary, the implanted depth must be corrected before the optical fiber is glued to the skull with a UV glue, because the fiber-based calcium signal may be contaminated by hemodynamic processes if the fiber is positioned, e.g., directly near a large blood vessel. 38 However, a hemodynamic contamination may be most problematic in widefield calcium imaging approaches as described in Valley et al. 39 An advantage of optical fiber-based calcium recordings represents the on-the-fly assessment of the data quality, i.e., the identification of the neuronal SoI, while implanting the fiber.…”