Towards multimodal nonlinear optical tomography - experimental methodology

Vogler, Nadine; Medyukhina, Anna; Latka, Ines; Kemper, Sebastian; Böhm, Markus; Dietzek, Benjamin; Popp, Jürgen

doi:10.1002/lapl.201110033

Cited by 47 publications

(44 citation statements)

References 59 publications

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“…the main component in lipids. Revealing the lipid distribution by CARS microscopy offers great potential for both ex vivo [44][45][46] and in vivo clinical diagnostics [47,48]. Aside from lipids other relevant molecules yield good CARS image contrast, e.g.…”

Section: Introductionmentioning

confidence: 99%

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Cicchi

Vogler

Kapsokalyvas

et al. 2012

Journal of Biophotonics

169

132

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Second‐harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and probing its hierarchical organization from molecular scale up to tissue architectural level. In fact, SHG combines the advantages of a non‐linear microscopy approach with a coherent modality able to probe molecular organization. In this manuscript we review the physical concepts describing SHG from collagen, highlighting how this optical process allows to probe structures ranging from molecular sizes to tissue architecture, through image pattern analysis and scoring methods. Starting from the description of the most relevant approaches employing SHG polarization anisotropy and forward – backward SHG detection, we then focus on the most relevant methods for imaging and characterizing collagen organization in tissues through image pattern analysis methods, highlighting advantages and limitations of the methods applied to tissue imaging and to potential clinical applications. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Introductionmentioning

confidence: 99%

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Cicchi

Vogler

Kapsokalyvas

et al. 2012

Journal of Biophotonics

169

132

View full text Add to dashboard Cite

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“…5 where CARS and SHG have been measured simultaneously on cardiomyocyte cells. CARS has also been used along with TPAF for skin imaging 137,138 or small organism imaging. 139 As several excitation sources are required for CARS, other nonlinear frequencies such as SFG can be implemented directly, and possibly simultaneously.…”

Section: Nonlinear Spectroscopy Multimodal Implementationsmentioning

confidence: 99%

Multimodal label-free microscopy

Pavillon

Fujita

Smith

2014

J. Innov. Opt. Health Sci.

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This paper reviews the di®erent multimodal applications based on a large extent of label-free imaging modalities, ranging from linear to nonlinear optics, while also including spectroscopic measurements. We put speci¯c emphasis on multimodal measurements going across the usual boundaries between imaging modalities, whereas most multimodal platforms combine techniques based on similar light interactions or similar hardware implementations. In this review, we limit the scope to focus on applications for biology such as live cells or tissues, since by their nature of being alive or fragile, we are often not free to take liberties with the image acquisition times and are forced to gather the maximum amount of information possible at one time. For such samples, imaging by a given label-free method usually presents a challenge in obtaining su±cient optical signal or is limited in terms of the types of observable targets. Multimodal imaging is then particularly attractive for these samples in order to maximize the amount of measured information. While multimodal imaging is always useful in the sense of acquiring additional information from additional modes, at times it is possible to attain information that could not be discovered using any single mode alone, which is the essence of the progress that is possible using a multimodal approach.

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“…The SHG images used both for training and validation of the herein presented algorithm were recorded as an integral part of the multimodal imaging approach described in detail by Vogler and coworkers [38]. In contrast to CARS highlighting the spatial distribution of lipids within the sections [39,40] and TPF, which displays the presence of autofluorophores within the sample, the SHG images used here highlight highly ordered structures lacking inversion symmetry in the sample.…”

Section: Multimodal Imagingmentioning

confidence: 99%

Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin

Medyukhina

Vogler

Latka

et al. 2011

Journal of Biophotonics

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All-optical microspectroscopic and tomographic tools have a great potential for the clinical investigation of human skin and skin diseases. However, automated optical tomography or even microscopy generate immense data sets. Therefore, in order to implement such diagnostic tools into the medical practice in both hospitals and private practice, there is a need for automated data handling and image analysis ideally implementing automized scores to judge the physiological state of a tissue section. In this contribution, the potential of an image processing algorithm for the automated classification of skin into normal or keloid based on second-harmonic generation (SHG) microscopic images is demonstrated. Such SHG data is routinely recorded within a multimodal imaging approach. The classification of the tissue implemented in the algorithm employs the geometrical features of collagen patterns that differ depending on the constitution, i.e., physiological status of the skin.

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Towards multimodal nonlinear optical tomography - experimental methodology

Cited by 47 publications

References 59 publications

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

From molecular structure to tissue architecture: collagen organization probed by SHG microscopy

Multimodal label-free microscopy

Automated classification of healthy and keloidal collagen patterns based on processing of SHG images of human skin

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