Towards marine biorefineries: Selective proteins extractions from marine macroalgae Ulva with pulsed electric fields

Polikovsky, Mark; Fernand, Francois; Sack, Martin; Frey, Wolfgang; Müller, Georg; Golberg, Alexander

doi:10.1016/j.ifset.2016.03.013

Cited by 85 publications

(50 citation statements)

References 34 publications

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“…This approach increased protein extract from 2.25 to 5.38% (w/v) for the untreated sample and the optimized method, respectively. A similar yield of protein from the same species was reported when using PEF combined with hydraulic pressure (Table 2) [44]. Another study reported the application of PEF with a custom-made insulated gate bipolar transistor-pulsed generator coupled with a gravitation press-electrode to aid protein extraction from U. ohnoi [45].…”

Section: Pulsed Electric Fieldsupporting

confidence: 64%

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

et al. 2020

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Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed Seaweed (macroalgae) is considered as a sustainable bioresource rich in high-quality nutrients such as protein. Seaweed protein can be used as an alternative to other protein sources. Furthermore, these proteins are natural reservoirs of bioactive peptides (BAPs) associated with various health benefits such as antioxidant, antihypertensive, and antidiabetic activities. However, seaweed-derived BAPs remain underexploited due to challenges that arise during protein extraction from algal biomass. Coupled with this, limited proteomic information exists regarding certain seaweed species. This review highlights the current state of the art of seaweed protein extraction techniques, e.g., liquid, ultrasound, microwave, pulsed electric field, and high hydrostatic pressure assisted extraction. The review also focuses on the enzymatic hydrolysis of seaweed proteins and characterization of the resultant hydrolysates/peptides using electrophoretic and chromatographic techniques. This includes reference to methods employed for separation, fractionation, and purification of seaweed BAPs, as well as the methodologies used for identification, e.g., analysis by mass spectrometry. Furthermore, a bioinformatics or in silico approach to aid discovery of seaweed BAPs is discussed herein. Based on the information available to date, it is suggested that further research is required in this area for the development of seaweed BAPs for nutraceutical applications.

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Section: Pulsed Electric Fieldsupporting

confidence: 64%

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

et al. 2020

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“…was chosen as the model species as it is very common on the shores of Israel and displayed high biomass productivity in extensive cultivation offshore in Israeli waters . Furthermore, the production of proteins and starch, and biomass fermentation to acetone, ethanol, butanol, and polyhydroxyalkanoates from several Ulva species has already been demonstrated …”

Section: Introductionmentioning

confidence: 99%

Feasibility study of Ulva sp. (Chlorophyta) intensive cultivation in a coastal area of the Eastern Mediterranean Sea

Chemodanov

Robin

Jinjikhashvily

et al. 2019

Biofuels Bioprod Bioref

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Increasing biomass production yields is a critical challenge for macroalgae biorefineries. The continuous tumbling and mixing of free‐floating algae through water or airflow has been shown to increase the productivity of algae in land‐based cultivation systems. This approach has not been tested thoroughly in offshore cultivation. We report, here, a field feasibility study on the increase in green macroalga Ulva sp. growth rates in offshore cages, achieved by the combined effect of tumbling and mixing of the algae using influxes of water and air. The experimental system was tested in a shallow coastal area in central Israel, in the eastern Mediterranean Sea. A maximum daily growth rate of 19.2%, areal productivity of 33.72 g dry weight (DW) day−1 m−2, and volumetric yields of 37.78 g DW day−1 m−3, together with 38.47 ± 0.01% ash and 5.28% protein content on a dry matter basis were achieved in the cages with intensified cultivation in the first week of May 2017. Our study shows that cultivation with tumbling and mixing of biomass with air, and water exchange with the environment is a feasible method to increase Ulva sp. biomass productivity offshore. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Current methods of breaking up the macroalgal cell wall to extract valuable compounds are not optimal and have disadvantages of high energy consumption in case of mechanical methods, harsh conditions and toxicity during chemical extraction, and generation of side products and waste if high temperatures are used [17][18][19]. In contrast, it has been demonstrated that enzyme-assisted extraction holds big potential for development of targeted and sustainable processing of seaweeds [2,3,20,21].…”

Section: Introductionmentioning

confidence: 99%

Macroalgae Derived Fungi Have High Abilities to Degrade Algal Polymers

et al. 2019

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Marine fungi associated with macroalgae are an ecologically important group that have a strong potential for industrial applications. In this study, twenty-two marine fungi isolated from the brown seaweed Fucus sp. were examined for their abilities to produce algal and plant biomass degrading enzymes. Growth of these isolates on brown and green algal biomass revealed a good growth, but no preference for any specific algae. Based on the analysis of enzymatic activities, macroalgae derived fungi were able to produce algae specific and (hemi-)cellulose degrading enzymes both on algal and plant biomass. However, the production of algae specific activities was lower than the production of cellulases and xylanases. These data revealed the presence of different enzymatic approaches for the degradation of algal biomass by macroalgae derived fungi. In addition, the results of the present study indicate our poor understanding of the enzymes involved in algal biomass degradation and the mechanisms of algal carbon source utilization by marine derived fungi.

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Towards marine biorefineries: Selective proteins extractions from marine macroalgae Ulva with pulsed electric fields

Cited by 85 publications

References 34 publications

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

Feasibility study of Ulva sp. (Chlorophyta) intensive cultivation in a coastal area of the Eastern Mediterranean Sea

Macroalgae Derived Fungi Have High Abilities to Degrade Algal Polymers

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