Towards manipulation of post-biosynthetic events in secondary metabolism of plant cell cultures

Purification and characterization of UDPG:ginsenoside Rd glucosyltransferase from suspended cells of Panax notoginseng

“…Glucosyltransferases transfer glucosyl groups from the donor to the hydroxyl groups of acceptors. In general, glucosyltransferases that use secondary metabolites as substrates are minor constituent proteins in plant cells [13] and play important roles in postbiosynthetic events in plant secondary metabolism [14,15]. There are reports on the purification of glucosyltransferases catalyzing the glucosylation of hydroxyl groups of compounds like flavonoid and phenols [16][17][18], however, as far as we know, no ginsenoside glucosyltransferases have been purified.…”

Assessment of genetic and epigenetic variation during long-term Taxus cell culture

“…Among these methods, plant cell culture using well-established procedures is regarded as an environmentally friendly option obtaining a highly pure product. It has met with some commercial success, for example in the case of Shikonin, Ginsenoside, Ajmalicine (Zhang et al 2002). Cell culture of Taxus is a promising, reliable, and long-term alternative for the production of paclitaxel and analogue compounds (Georgiev et al 2009).…”

Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy

“…If the cells are to remain intact, the intracellularly stored metabolites may require cell permeabilization [4,12], but possibly at the expense of cell viability and complete destruction of biomass. Upon release, substance volatility and product dispersion at high dilution in the culture entails concentrating the broth prior to further purification [5,20,28]. Ultimately, the time and expenses in regenerating the biomass and downstream processing, including the wastewater treatment, may render the bioprocesses economically unattractive.…”