Towards high-temperature fuel ethanol production using Kluyveromyces marxianus: On the search for plug-in strains for the Brazilian sugarcane-based biorefinery

Madeira-Jr, José Valdo; Gombert, Andreas

doi:10.1016/j.biombioe.2018.09.010

Cited by 45 publications

(32 citation statements)

References 62 publications

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“…Furthermore, this trait allows fermentations at high cell density, which may bring cost benefits to the process, especially if cell recycling can be considered. The excellent tolerance of the K. marxianus PYCC 3286 strain to ethanol has been recently noted by other authors who showed that this strain can tolerate up to 79 g/L of ethanol [43]. Moreover, a high beta-galactosidase specific activity was also reported for this strain (3207 IU/g DW) [44].…”

Section: Resultssupporting

confidence: 59%

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

et al. 2019

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Dairy industries have a high environmental impact, with very high energy and water consumption and polluting effluents. To increase the sustainability of these industries it is urgent to implement technologies for wastewater treatment allowing water recycling and energy savings. In this study, dairy wastewater was processed by ultrafiltration and nanofiltration or ultrafiltration and reverse osmosis (UF/RO) and retentates from the second membrane separation processes were assessed for bioenergy production. Lactose-fermenting yeasts were tested in direct conversion of the retentates (lactose-rich streams) into bioethanol. Two Kluyveromyces strains efficiently fermented all the lactose, with ethanol yields higher than 90% (>0.47 g/g yield). Under severe oxygen-limiting conditions, the K. marxianus PYCC 3286 strain reached 70 g/L of ethanol, which is compatible with energy-efficient distillation processes. In turn, the RO permeate is suitable for recycling into the cleaning process. The proposed integrated process, using UF/RO membrane technology, could allow water recycling (RO permeate) and bioenergy production (from RO retentate) for a more sustainable dairy industry.

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Section: Resultssupporting

confidence: 59%

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

et al. 2019

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“…Like other non-conventional microbes, there has been considerably less research effort put towards developing well-characterized genetic parts for manipulating gene expression in comparison to the common laboratory host and ethanol producer Saccharomyces cerevisiae ( Löbs et al., 2017 ). Despite this general lack of metabolic engineering tools, simple genetic manipulations and media optimization have been used to demonstrate the potential of K. marxianus for a variety of bioprocesses including protein production, high temperature ethanol fermentation, the biosynthesis of ethyl acetate (a native metabolite produced in grams per liter quantities in many K. marxianus strains) and heterologous products such as the polyketide triacetic acid lactone (TAL) ( Cernak et al., 2018 ; Raimondi et al., 2013 ; Kushi et al., 2000 ; Lertwattanasakul et al., 2011 ; Yarimizu et al., 2015 ; Madeira and Gombert, 2018 ; McTaggart et al., 2019 ). The development of new metabolic engineering tools ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Developing a broad-range promoter set for metabolic engineering in the thermotolerant yeast Kluyveromyces marxianus

Lang

Besada‐Lombana

et al. 2020

Metabolic Engineering Communications

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Kluyveromyces marxianus is an emerging host for metabolic engineering. This thermotolerant yeast is the fastest growing eukaryote, has high flux through the TCA cycle, and can metabolize a broad range of C5, C6, and C12 carbon sources. In comparison to the common host Saccharomyces cerevisiae , this non-conventional yeast suffers from a lack of metabolic engineering tools to control gene expression over a wide transcriptional range. To address this issue, we designed a library of 25 native-derived promoters from K. marxanius CBS6556 that spans 87-fold transcriptional strength under glucose metabolism. Six promoters from the library were further characterized in both glucose and xylose as well as across various temperatures from 30 to 45 °C. The temperature study revealed that in most cases EGFP expression decreased with elevating temperature; however, two promoters, P SSA3 and P ADH1 , increased expression above 40 °C in both xylose and glucose. The six-promoter set was also validated in xylose for triacetic acid lactone (TAL) production. By controlling the expression level of heterologous 2-pyrone synthase (2-PS), the specific TAL titer increased over 8-fold at 37 °C. Cultures at 41 °C exhibited a similar TAL biosynthesis capability, while at 30 °C TAL levels were lower. Taken together, these results advance the metabolic engineering tool set in K. marxianus and further develop this new host for chemical biosynthesis.

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“…There have been several studies on sugar utilization and ethanol production by K . marxianus at high temperatures 1,2,4,5 that were carried out with the aim of establishing high-temperature fermentation, which has advantages including reduction of cooling costs, prevention of contamination and reduction of enzymatic hydrolysis cost 5–7 . The regulation of some genes related to glucose repression in K .…”

Section: Introductionmentioning

confidence: 99%

MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus

Nurcholis

Murata

Limtong

et al. 2019

Sci Rep

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Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype. Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1 . Consistently, introduction of HIS4 into Kmmig1 suppressed the requirement of histidine. Considering the fact that His4 catalyzes four of ten steps in histidine biosynthesis, K . marxianus has evolved a novel and effective regulation mechanism via Mig1 for the control of histidine biosynthesis. Moreover, RNA-Seq analysis revealed that there were more than 1,000 differentially expressed genes in Kmmig1 , suggesting that Mig1 is directly or indirectly involved in the regulation of their expression as a global regulator.

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Towards high-temperature fuel ethanol production using Kluyveromyces marxianus: On the search for plug-in strains for the Brazilian sugarcane-based biorefinery

Cited by 45 publications

References 62 publications

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

Developing a broad-range promoter set for metabolic engineering in the thermotolerant yeast Kluyveromyces marxianus

MIG1 as a positive regulator for the histidine biosynthesis pathway and as a global regulator in thermotolerant yeast Kluyveromyces marxianus

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