“…Every plate was dedicated to testing a specific cadmium concentration (32, 70, 155, 341, and 750 µg/L of Cadmium), with one plate designated for the control group. Cadmium was chosen as the positive test compound due to its well-studied nature in Biosystems and environment [2] , [3] , [4] and the model's proven sensitivity to the compound. It was also used as reference in the Lyamnea stagnalis OECD reproductive test guideline working as a reference compound for the test development, providing the basis to define the models sensitivity in the current testing conditions when compared to the available test guidelines and models [ 1 , [5] , [6] , [7] ].…”